Growth of Staphylococcus aureus with defective siderophore production in human peritoneal dialysate solution.

In this study, we attempted to determine the effects of iron-availability and the activity of the bacterial iron-uptake system (IUS) on the growth of Staphylococcus aureus in human peritoneal dialysate (HPD) solution. A streptonigrin-resistant S. aureus (SRSA) strain, isolated from S. aureus ATCC 6538, exhibited defective siderophore production, thereby resulting in ineffective uptake of iron from low iron-saturated transferrin. The growth of both strains was stimulated in HPD solution supplemented with FeCl3 and holotransferrin, but growth was inhibited in HPD solution which had been supplemented with apotransferrin and dipyridyl. The SRSA strain grew less robustly than did its parental strain in both iron-supplemented HPD solution and regular HPD solution. These results indicate that iron-availability and siderophore-mediated IUS activity in particular, the ability to produce siderophores and thus capture iron from low iron-saturated transferrin play critical roles in the growth of S. aureus in HPD solution. Our results also indicated that the possibility of using iron chelators as therapeutic or preventive agents warrants further evaluation.

[1]  G. Lajoie,et al.  Role of Siderophore Biosynthesis in Virulence of Staphylococcus aureus: Identification and Characterization of Genes Involved in Production of a Siderophore , 2004, Infection and Immunity.

[2]  Sung-Heui Shin,et al.  Growth and siderophore production of staphylococci in human peritoneal dialysate. , 2003, Journal of Korean medical science.

[3]  Eric P. Skaar,et al.  Passage of Heme-Iron Across the Envelope of Staphylococcus aureus , 2003, Science.

[4]  D. Heinrichs,et al.  Identification and Characterization offhuD1 and fhuD2, Two Genes Involved in Iron-Hydroxamate Uptake in Staphylococcus aureus , 2001, Journal of bacteriology.

[5]  A. Rouhiainen,et al.  Dependence of Staphylococcus epidermidis on non-transferrin-bound iron for growth. , 2001, FEMS microbiology letters.

[6]  J. Rhee,et al.  CAS agar diffusion assay for the measurement of siderophores in biological fluids. , 2001, Journal of microbiological methods.

[7]  R. Jayaswal,et al.  Molecular Characterization of the Iron-Hydroxamate Uptake System in Staphylococcus aureus , 2001, Applied and Environmental Microbiology.

[8]  A. Tomasz,et al.  The development of vancomycin resistance in a patient with methicillin-resistant Staphylococcus aureus infection. , 1999, The New England journal of medicine.

[9]  R. Courcol,et al.  Iron depletion and virulence in Staphylococcus aureus. , 1996, FEMS microbiology letters.

[10]  P. Williams,et al.  Staphylococci express a receptor for human transferrin: identification of a 42-kilodalton cell wall transferrin-binding protein , 1994, Infection and immunity.

[11]  R. Gaynes,et al.  An overview of nosocomial infections, including the role of the microbiology laboratory , 1993, Clinical Microbiology Reviews.

[12]  A. von Graevenitz,et al.  Microbiological aspects of peritonitis associated with continuous ambulatory peritoneal dialysis , 1992, Clinical Microbiology Reviews.

[13]  U. Seal,et al.  The detection of four molecular forms of human transferrin during the iron binding process. , 1976, Biochimica et biophysica acta.

[14]  C. Ratledge,et al.  Iron metabolism in pathogenic bacteria. , 2000, Annual review of microbiology.

[15]  B. Sadowska,et al.  The virulence of Staphylococcus aureus isolates differing by siderophore production. , 1998, Acta microbiologica Polonica.