Biohydrogen production from dark fermentation of cheese whey: Influence of pH

Abstract Hydrogen production from cheese whey through dark fermentation was investigated in this study in order to systematically analyse the effects of the operating pH. The effluents from pecorino cheese and mozzarella cheese production were the substrates used for the fermentation tests. Either CW only or a mixture of CW and heat-shocked activated sludge were used in mesophilic pH-controlled batch fermentation experiments. The results indicated that hydrogen production was strongly affected by multiple factors including the substrate characteristics, the addition of an inoculum as well as the pH. The process variables were found to affect to a varying extent numerous interrelated aspects of the fermentation process, including the hydrogen production potential, the type of fermentation pathways, as well as the process kinetics. The fermentation products varied largely with the operating conditions and mirrored the H2 yield. Significant fermentative biohydrogen production was attained at pHs of 6.5–7.5, with the best performance in terms of H2 generation potential (171.3 NL H2/kg TOC) being observed for CW from mozzarella cheese production, at a pH value of 6.0 with the heat-shocked inoculum.

[1]  O. Yenigün,et al.  Anaerobic treatment of dairy wastewaters: a review , 2005 .

[2]  Irini Angelidaki,et al.  Homoacetogenesis as the alternative pathway for H2 sink during thermophilic anaerobic degradation of butyrate under suppressed methanogenesis. , 2007, Water research.

[3]  Craig S. Criddle,et al.  Parallel Processing of Substrate Correlates with Greater Functional Stability in Methanogenic Bioreactor Communities Perturbed by Glucose , 2000, Applied and Environmental Microbiology.

[4]  Michael Kornaros,et al.  Using cheese whey for hydrogen and methane generation in a two-stage continuous process with alternative pH controlling approaches. , 2009, Bioresource technology.

[5]  A Polettini,et al.  An experimental study on fermentative H₂ production from food waste as affected by pH. , 2014, Waste management.

[6]  Hang-Sik Shin,et al.  Effect of initial pH independent of operational pH on hydrogen fermentation of food waste. , 2011, Bioresource technology.

[7]  Gang Wang,et al.  Response surface methodological analysis on biohydrogen production by enriched anaerobic cultures , 2006 .

[8]  C. Alais.  Science du lait et principes des techniques laitières , 1964 .

[9]  M. Zaiat,et al.  Hydrogen production in an upflow anaerobic packed bed reactor used to treat cheese whey , 2013 .

[10]  Bulent Keskinler,et al.  Cheese whey treatment performance of an aerobic jet loop membrane bioreactor , 2004 .

[11]  Luis Manuel Rosales-Colunga,et al.  Continuous biohydrogen production using cheese whey: Improving the hydrogen production rate , 2009 .

[12]  L. Borzacconi,et al.  Feasibility of biohydrogen production from cheese whey using a UASB reactor: Links between microbial community and reactor performance , 2009 .

[13]  Ruihong Zhang,et al.  Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities , 2007 .

[14]  H. H. Fang,et al.  Acidogenesis of dairy wastewater at various pH levels. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[15]  G. Antonopoulou,et al.  Biofuels generation from sweet sorghum: fermentative hydrogen production and anaerobic digestion of the remaining biomass. , 2008, Bioresource technology.

[16]  Gerasimos Lyberatos,et al.  Production of Gaseous Biofuels and Electricity from Cheese Whey , 2011 .

[17]  Mohd Ali Hassan,et al.  Microbial characterization of hydrogen-producing bacteria in fermented food waste at different pH va , 2011 .

[18]  A. Aivasidis,et al.  Two-stage CSTR–UASB digestion enables superior and alkali addition-free cheese whey treatment , 2014 .

[19]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[20]  H. Steinmetz,et al.  Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics , 2012 .

[21]  Hong Liu,et al.  Effect of pH on hydrogen production from glucose by a mixed culture. , 2002, Bioresource technology.

[22]  Michael Kornaros,et al.  Biohydrogen and methane production from cheese whey in a two-stage anaerobic process , 2008 .

[23]  Jean-Philippe Steyer,et al.  Hydrogen production from agricultural waste by dark fermentation: A review , 2010 .

[24]  D L Hawkes,et al.  Performance characteristics of a two‐stage dark fermentative system producing hydrogen and methane continuously , 2007, Biotechnology and bioengineering.

[25]  F. Kargı,et al.  Ethanol production from cheese whey powder solution in a packed column bioreactor at different hydraulic residence times , 2008 .

[26]  Han-Qing Yu,et al.  Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures , 2002 .

[27]  Jean-Paul Schwitzguébel,et al.  Hydrogen production by Clostridium thermolacticum during continuous fermentation of lactose , 2004 .

[28]  M. Błaszczyk,et al.  Lactic Acid Bacteria in Hydrogen-Producing Consortia: On Purpose or by Coincidence? , 2017 .

[29]  R. Conrad.  Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments , 1999 .

[30]  M. Siso.  The biotechnological utilization of cheese whey: A review , 1996 .

[31]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[32]  Luis Manuel Rosales-Colunga,et al.  Fermentation of lactose and its constituent sugars by Escherichia coli WDHL: impact on hydrogen production. , 2012, Bioresource technology.

[33]  D. Bagley,et al.  Modeling Microbial Kinetics in an Anaerobic Sequencing Batch Reactor ‐ Model Development and Experimental Validation , 1999 .

[34]  J. M. Kongo.  Lactic Acid Bacteria - R & D for Food, Health and Livestock Purposes , 2013 .

[35]  Juan M. Lema,et al.  Influence of the content in fats and proteins on the anaerobic biodegradability of dairy wastewaters , 2000 .

[36]  N. Azbar,et al.  Optimization of Basal Medium for Fermentative Hydrogen Production from Cheese Whey Wastewater , 2009 .

[37]  Herbert H. P. Fang,et al.  Fermentative Hydrogen Production From Wastewater and Solid Wastes by Mixed Cultures , 2007 .

[38]  K. Korkmaz,et al.  Comparative Evaluation of Bio-Hydrogen Production From Cheese Whey Wastewater Under Thermophilic and Mesophilic Anaerobic Conditions , 2009 .

[39]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[40]  Vandré Barbosa Brião,et al.  Effluent generation by the dairy industry: preventive attitudes and opportunities , 2007 .

[41]  Samir Kumar Khanal,et al.  Biological hydrogen production: effects of pH and intermediate products , 2003 .

[42]  Marcelo Zaiat,et al.  Application of an anaerobic packed-bed bioreactor for the production of hydrogen and organic acids , 2008 .

[43]  J. Rivas,et al.  Cheese whey wastewater: characterization and treatment. , 2013, The Science of the total environment.

[44]  K. Pinder,et al.  Acidogenic fermentation of lactose , 1987, Biotechnology and bioengineering.

[45]  N. Saady.  Homoacetogenesis during hydrogen production by mixed cultures dark fermentation: Unresolved challenge , 2013 .

[46]  Lynne E. Macaskie,et al.  Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy , 2009 .

[47]  Luis Manuel Rosales-Colunga,et al.  Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate , 2010 .

[48]  I. Valdez‐Vazquez,et al.  Hydrogen production by fermentative consortia , 2009 .

[49]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[50]  Gustavo Davila-Vazquez,et al.  Fermentative hydrogen production in batch experiments using lactose, cheese whey and glucose: Influence of initial substrate concentration and pH , 2008 .

[51]  K. Korkmaz,et al.  Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions , 2009 .

[52]  Edson Luiz Silva,et al.  Different ratios of carbon sources in the fermentation of cheese whey and glucose as substrates for hydrogen and ethanol production in continuous reactors , 2014 .

[53]  A. Tilche,et al.  Anaerobic treatment of cheese whey with a downflow-upflow hybrid reactor , 1996 .

[54]  J. Rivas,et al.  Cheese whey management: a review. , 2012, Journal of environmental management.

[55]  Moonhyun Hwang,et al.  Anaerobic bio-hydrogen production from ethanol fermentation: the role of pH. , 2004, Journal of biotechnology.