Characterization of the Bacillus subtilis YxdJ response regulator as the inducer of expression for the cognate ABC transporter YxdLM.

The genome of Bacillus subtilis, like those of some other AT-rich Gram-positive bacteria, has the uncommon feature of containing several copies of arrangements in which the genes encoding two-component and cognate ABC transporter systems are adjacent. As the function of one of these systems, the product of the yxd locus, is still unknown, it was analysed further in order to get some clues on the physiological role of the gene products it encodes. The yxdJ gene was shown to encode a DNA-binding protein that directly controls transcription of the neighbouring operon encoding the ABC transporter YxdLM. Primer extension and DNase protection experiments allowed precise definition of the yxdLM transcription start and controlling region. Two putative direct repeats were identified that are proposed to be the YxdJ response regulator binding sites. Whole-cell transcriptome analyses revealed that the YxdJ regulon is extremely restricted. In addition to the yxdJKLMyxeA operon, only a few genes involved in modifications of the bacterial cell wall were shown to be regulated by YxdJ.

[1]  J. Tagg,et al.  Intra- and Interspecies Signaling betweenStreptococcus salivarius and Streptococcus pyogenes Mediated by SalA and SalA1 Lantibiotic Peptides , 2001, Journal of bacteriology.

[2]  S Brunak,et al.  Sigma A recognition sites in the Bacillus subtilis genome. , 2001, Microbiology.

[3]  M. Kuroda,et al.  Identification of the up- and down-regulated genes in vancomycin-resistant Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential hybridization method. , 2000, Biochemical and biophysical research communications.

[4]  J. S. Parkinson,et al.  Communication modules in bacterial signaling proteins. , 1992, Annual review of genetics.

[5]  Tao Wang,et al.  Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon , 2003, Molecular microbiology.

[6]  M. Baptista,et al.  Attenuated virulence of Streptococcus agalactiae deficient in D‐alanyl‐lipoteichoic acid is due to an increased susceptibility to defensins and phagocytic cells , 2003, Molecular microbiology.

[7]  W. Fischer,et al.  The absence of D-alanine from lipoteichoic acid and wall teichoic acid alters surface charge, enhances autolysis and increases susceptibility to methicillin in Bacillus subtilis. , 1997, Microbiology.

[8]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[9]  Naotake Ogasawara,et al.  The BceRS two‐component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis , 2003, Molecular microbiology.

[10]  I. J. Evans,et al.  A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria , 1986, Nature.

[11]  P. Joseph,et al.  YtsCD and YwoA, two independent systems that confer bacitracin resistance to Bacillus subtilis. , 2003, FEMS microbiology letters.

[12]  C. Anagnostopoulos,et al.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS , 1961, Journal of bacteriology.

[13]  Y. Fujita,et al.  Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome. , 2000, Microbiology.

[14]  Naotake Ogasawara,et al.  Comprehensive DNA Microarray Analysis ofBacillus subtilis Two-Component Regulatory Systems , 2001, Journal of bacteriology.

[15]  J. Spizizen,et al.  TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. , 1958, Proceedings of the National Academy of Sciences of the United States of America.

[16]  M. Inouye,et al.  Identification of the DNA-binding domain of the OmpR protein required for transcriptional activation of the ompF and ompC genes of Escherichia coli by in vivo DNA footprinting. , 1989, The Journal of biological chemistry.

[17]  K. Makino,et al.  Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro. , 1988, Journal of molecular biology.

[18]  J. Lee,et al.  Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis , 1994, Journal of bacteriology.

[19]  H. Kalbacher,et al.  Inactivation of the dlt Operon inStaphylococcus aureus Confers Sensitivity to Defensins, Protegrins, and Other Antimicrobial Peptides* , 1999, The Journal of Biological Chemistry.

[20]  K. Kobayashi,et al.  The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis. , 2000, Microbiology.

[21]  F M Hulett,et al.  Three two‐component signal‐transduction systems interact for Pho regulation in Bacillus subtilis , 1996, Molecular microbiology.

[22]  G. Fichant,et al.  Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. , 1999, Journal of molecular biology.

[23]  S. Brunak,et al.  SHORT COMMUNICATION Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites , 1997 .

[24]  H. Tsuda,et al.  Genes Involved in Bacitracin Resistance in Streptococcus mutans , 2002, Antimicrobial Agents and Chemotherapy.

[25]  Characterization of OmpR binding sequences in the upstream region of the ompF promoter essential for transcriptional activation. , 1989, The Journal of biological chemistry.

[26]  M. Perego,et al.  Incorporation of D-Alanine into Lipoteichoic Acid and Wall Teichoic Acid in Bacillus subtilis , 1995, The Journal of Biological Chemistry.

[27]  F. Fiedler,et al.  Regulation of d-Alanyl-Lipoteichoic Acid Biosynthesis in Streptococcus agalactiae Involves a Novel Two-Component Regulatory System , 2001, Journal of bacteriology.

[28]  J. D. Helmann,et al.  Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA , 1995, Nucleic Acids Res..

[29]  G. F. Ames.  Bacterial periplasmic transport systems: structure, mechanism, and evolution. , 1986, Annual review of biochemistry.

[30]  P. Joseph,et al.  Regulatory relationship of two-component and ABC transport systems and clustering of their genes in the Bacillus/Clostridium group, suggest a functional link between them. , 2002, Journal of molecular microbiology and biotechnology.

[31]  G. Weinstock,et al.  Anaerobic control of colicin E1 production , 1992, Journal of bacteriology.

[32]  Victoria A. Feher,et al.  Two-Component Signal Transduction in Bacillus subtilis: How One Organism Sees Its World , 1999, Journal of bacteriology.

[33]  D. Belin,et al.  Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter , 1995, Journal of bacteriology.

[34]  P. Joseph,et al.  Rapid orientated cloning in a shuttle vector allowing modulated gene expression in Bacillus subtilis. , 2001, FEMS microbiology letters.