Lipid fatty acid composition and oxidative susceptibility in eggs of hens fed a fish fat diet supplemented with vitamin E,C, or synthetic antioxidant

Hy-Line laying hens from weeks 24 to 67 of age were fed a 0.3% fish fat diet with or without vitamin E, ascorbic acid, or synthetic antioxidants. Lipid composition, cholesterol and α-tocopherol levels and degree of fat oxidation (TBA-RS) in yolk during storage were investigated. Dietary fish fat led to an increased n-3 PUFA content in yolk lipids (from 14.4 to 21.5 mg kg-1) and to a lower n-6: n-3 PUFA ratio (from 7.75 to 4.97) without any effect on the level of cholesterol in eggs. The supplements tested exerted no influence on the composition of egg fatty acids. The dietary vitamin E supplement increased α-tocopherol in egg yolk from 24.2 to 68.3 mg kg–1 and improved fat stability during storage for 15 and 42 days. Similar stabilizing properties were observed after supplementing the feed with synthetic antioxidants (BHT, BHA and EQ) and to a lesser degree when vitamin C was added.

[1]  N. Flanagan,et al.  Enriched eggs as a source of N-3 polyunsaturated fatty acids for humans. , 2000, Poultry science.

[2]  G. Manfreda,et al.  Effects of dietary vitamin E on the quality of table eggs enriched with n-3 long-chain fatty acids. , 2000, Poultry science.

[3]  J. Latshaw,et al.  α‐Tocopherol Content and Oxidative Stability of Egg Yolk as Related to Dietary α‐Tocopherol , 1998 .

[4]  D. Farrell.  Enrichment of hen eggs with n-3 long-chain fatty acids and evaluation of enriched eggs in humans. , 1998, The American journal of clinical nutrition.

[5]  G. Qi,et al.  Natural Tocopherol Enrichment and Its Effect in n−3 Fatty Acid Modified Chicken Eggs† , 1998 .

[6]  M. Kuchta,et al.  Comparison of the influence of rapeseed oil and fish fat in laying hen nutrition on the level of polyunsaturated fatty acids in egg yolk , 1998 .

[7]  M. Kuchta,et al.  The influence of marine originated fat in diet for hens on the polyunsaturated fatty acids in egg yolk and cholesterol content in blood serum , 1998 .

[8]  J. Sim,et al.  Dietary oils with added tocopherols: effects on egg or tissue tocopherols, fatty acids, and oxidative stability. , 1996, Poultry science.

[9]  K. Pienta,et al.  The effects of omega-3 and omega-6 fatty acids on in vitro prostate cancer growth. , 1996, Anticancer research.

[10]  M. V. Van Elswyk,et al.  Yolk thiobarbituric acid reactive substances and n-3 fatty acids in response to whole and ground flaxseed. , 1995, Poultry science.

[11]  W. Robey.  The damaging effects of oxidation , 1994 .

[12]  M. V. Van Elswyk,et al.  Manipulating the fatty acid composition of poultry meat and eggs for the health conscious consumer , 1993 .

[13]  H. Griffin.  Manipulation of egg yolk cholesterol: a physiologist's view , 1992 .

[14]  A. Simopoulos.  Omega-3 fatty acids in health and disease and in growth and development. , 1991, The American journal of clinical nutrition.

[15]  A. Diplock.  Antioxidant nutrients and disease prevention: an overview. , 1991, The American journal of clinical nutrition.

[16]  Jan Pikul,et al.  Evaluation of three modified TBA methods for measuring lipid oxidation in chicken meat , 1989 .

[17]  P. Hargis.  Modifying egg yolk cholesterol in the domestic fowl—a review , 1988 .

[18]  G. Anderson,et al.  The essentiality of n-3 fatty acids for the development and function of the retina and brain. , 1988, Annual review of nutrition.

[19]  Denise M Smith,et al.  Modified extraction 2-thiobarbituric acid method for measuring lipid oxidation in poultry. , 1987, Poultry science.

[20]  U. Manz,et al.  A method for the routine determination of tocopherols in animal feed and human foodstuffs with the aid of high performance liquid chromatography. , 1981, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[21]  H. Sinclair.  DIETARY FATS AND CORONARY HEART DISEASE , 1980, The Lancet.

[22]  R. V. D. Merwe,et al.  Flavour studies with fish meals and with fish oil fractions in broiler diets , 1973 .

[23]  K. C. Leong,et al.  Effect of Feeding and Withdrawal of Menhaden Oil on the w3 and w6 Fatty Acid Content of Broiler Tissues , 1969 .