Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2.

The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV-vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy. The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure. Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances. The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area. The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02-6.0 mmol·L-1 with a low detection limit of 12.4 μmol·L-1, good anti-interference ability and long-term storage stability. The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.

[1]  Xiaojuan Liu,et al.  Preparation and electrochemical investigation of a nano-structured material Ni2+/MgFe layered double hydroxide as a glucose biosensor , 2011 .

[2]  E. Samulski,et al.  Synthesis of water soluble graphene. , 2008, Nano letters.

[3]  Hong‐Yan Zeng,et al.  Ultrafine hydrotalcite particles prepared with novel technology to improve the flame retardancy of polypropylene , 2015 .

[4]  S. Berchmans,et al.  A Sensing Platform for Direct Electron Transfer Study of Horseradish Peroxidase , 2013 .

[5]  Xiaoling Yang,et al.  A novel hydrogen peroxide biosensor based on Au-graphene-HRP-chitosan biocomposites , 2010 .

[6]  Jinping Liu,et al.  A novel glucose sensor based on monodispersed Ni/Al layered double hydroxide and chitosan. , 2008, Biosensors & bioelectronics.

[7]  Lin Hongwei,et al.  A novel H2O2 biosensor based on Fe3O4–Au magnetic nanoparticles coated horseradish peroxidase and graphene sheets–Nafion film modified screen-printed carbon electrode , 2013 .

[8]  A Pawlak,et al.  Thermogravimetric and FTIR studies of chitosan blends , 2003 .

[9]  Lin Zhang,et al.  Immobilization of carbonic anhydrase by embedding and covalent coupling into nanocomposite hydrogel containing hydrotalcite , 2009 .

[10]  Linfield Brown,et al.  Limit of Detection , 2002 .

[11]  Qun Huang,et al.  Study on the synergetic degradation of chitosan with ultraviolet light and hydrogen peroxide. , 2005, Carbohydrate research.

[12]  Qin Xu,et al.  Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite. , 2006, Biosensors & bioelectronics.

[13]  S. Cosnier,et al.  HRP/[Zn-Cr-ABTS] redox clay-based biosensor: design and optimization for cyanide detection. , 2004, Biosensors & bioelectronics.

[14]  Zhonghua Xue,et al.  Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively. , 2017, Biosensors & bioelectronics.

[15]  C. Mousty.  Sensors and biosensors based on clay-modified electrodes: new trends , 2004 .

[16]  K. Parida,et al.  Effect of Co2+ Substitution in the Framework of Carbonate Intercalated Cu/Cr LDH on Structural, Electronic, Optical, and Photocatalytic Properties , 2012 .

[17]  Zhibin Mai,et al.  Direct electrochemistry and electrocatalysis of horseradish peroxidase based on clay-chitosan-gold nanoparticle nanocomposite. , 2008, Biosensors & bioelectronics.

[18]  S. Meenakshi,et al.  Enriched fluoride sorption using alumina/chitosan composite. , 2010, Journal of hazardous materials.

[19]  Qun Chen,et al.  Copper Tetrasulfophthalocyanine Intercalated Hydrotalcite as an Efficient Bifunctional Catalyst for the Baeyer–Villiger Oxidation , 2016, Catalysis Letters.

[20]  Wensheng Yang,et al.  Direct electrochemistry and electrocatalysis based on a film of horseradish peroxidase intercalated into Ni-Al layered double hydroxide nanosheets. , 2008, Biosensors & bioelectronics.

[21]  Y. Chai,et al.  Amperometric hydrogen peroxide biosensor based on the immobilization of HRP on DNA–silver nanohybrids and PDDA-protected gold nanoparticles , 2009 .

[22]  Xin Deng,et al.  Preparation of Mg‐Al hydrotalcite by urea method and its catalytic activity for transesterification , 2009 .

[23]  Jing Han,et al.  Cycling stability of iron-based layered double hydroxide thin-films for battery-type electrode materials , 2017, Journal of Materials Science: Materials in Electronics.

[24]  Zhihong Lin,et al.  A europium-ion-based luminescent sensing probe for hydrogen peroxide. , 2002, Angewandte Chemie.

[25]  Yaping Ding,et al.  Amperometric glucose biosensor based on NiFe2O4 nanoparticles and chitosan , 2010 .

[26]  J. Kennedy,et al.  Preparation and properties of alginate/carboxymethyl chitosan blend fibers , 2006 .

[27]  Qian Zhu,et al.  A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells. , 2017, Biosensors & bioelectronics.

[28]  Yinling Wang,et al.  Horseradish peroxidase immobilization on carbon nanodots/CoFe layered double hydroxides: direct electrochemistry and hydrogen peroxide sensing. , 2015, Biosensors & bioelectronics.

[29]  S. Momeni,et al.  Colorimetric sensor assay for detection of hydrogen peroxide using green synthesis of silver chloride nanoparticles: Experimental and theoretical evidence , 2017 .

[30]  W. Long,et al.  The application of Zn–Al-hydrotalcite as a novel anodic material for Ni–Zn secondary cells , 2013 .

[31]  J. S. Beck,et al.  Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism , 1992, Nature.

[32]  Eduardo Ruiz-Hitzky,et al.  Chitosan–clay nanocomposites: application as electrochemical sensors , 2005 .

[33]  J. D. Winefordner,et al.  Limit of detection. A closer look at the IUPAC definition , 1983 .

[34]  Fabrizio Cavani,et al.  Hydrotalcite-type anionic clays: Preparation, properties and applications. , 1991 .

[35]  J. Tu,et al.  Influence of surface modification with Sn6O4(OH)4 on electrochemical performance of ZnO in Zn/Ni secondary cells , 2007 .

[36]  Yongqi Ding,et al.  Photochemical synthesis of glutathione-stabilized silver nanoclusters for fluorometric determination of hydrogen peroxide , 2017, Microchimica Acta.

[37]  J. Yvon,et al.  Corrosion inhibitors based on chitosan-heptanoate modified beidellite , 2012 .

[38]  S. Dong,et al.  Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol-gel-derived ceramic-carbon nanotube nanocomposite film. , 2007, Biosensors & bioelectronics.

[39]  K. Kano,et al.  Highly-sensitive flow injection determination of hydrogen peroxide with a peroxidase-immobilized electrode and its application to clinical chemistry , 2000 .

[40]  K. Sing.  Reporting physisorption data for gas/solid systems with special reference to the determination of surface area and porosity (Recommendations 1984) , 1985 .

[41]  Eduardo Ruiz-Hitzky,et al.  Biopolymer−Clay Nanocomposites Based on Chitosan Intercalated in Montmorillonite , 2003 .

[42]  J. Fierro,et al.  The fabrication and characterization of Cu-nanoparticle immobilization on a hybrid chitosan derivative-carbon support as a novel electrochemical sensor: application for the sensitive enzymeless oxidation of glucose and reduction of hydrogen peroxide. , 2014, Journal of materials chemistry. B.

[43]  Yaping Ding,et al.  Hydrogen peroxide biosensor based on horseradish peroxidase immobilized on chitosan-wrapped NiFe2O4 nanoparticles , 2011 .

[44]  Diana A. Centeno,et al.  A new peroxidase from leaves of guinea grass (Panicum maximum): A potential biocatalyst to build amperometric biosensors. , 2017, Bioelectrochemistry.

[45]  S. Dong,et al.  Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium. , 1999, Analytical chemistry.

[46]  Y. Mi,et al.  Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized on Fe3O4/Chitosan Modified Glassy Carbon Electrode , 2009 .

[47]  S. Cosnier,et al.  Hybrid material based on chitosan and layered double hydroxides: characterization and application to the design of amperometric phenol biosensor. , 2007, Biomacromolecules.

[48]  Ali H. Jawad,et al.  Photocatalytic-oxidation of solid state chitosan by immobilized bilayer assembly of TiO2–chitosan under a compact household fluorescent lamp irradiation , 2011 .

[49]  Yusuke Okawa,et al.  Enzyme monolayer- and bilayer-modified tin oxide electrodes for the determination of hydrogen peroxide and glucose , 1989 .