Position of chromosomes 18, 19, 21 and 22 in 3D-preserved interphase nuclei of human and gorilla and white hand gibbon

BackgroundEven though comparative nuclear architecture studies in hominoids are sparse, nuclear chromosome architecture was shown to be conserved during hominoid evolution. Thus, it is suspected that yet unknown biological mechanisms must underlie this observation.ResultsHere for the first time a combination of multicolor banding (MCB) and three-dimensional analysis of interphase cells was used to characterize the position and orientation of human chromosomes #18, #19, #21 and #22 and their homologues in primate B-lymphocytic cells. In general, our data is in concordance with previous studies. The position of the four studied human chromosomes and their homologues were conserved during primate evolution. However, comparison of interphase architecture in human B-lymphocytic cells and sperm revealed differences of localization of acrocentric chromosomes. The latter might be related to the fact that the nucleolus organizing region is not active in sperm.ConclusionStudies in different tissue types may characterize more – potentially biologically relevant differences in nuclear architecture.

[1]  S. Bhatt,et al.  Breakpoint characterization: a new approach for segregation analysis of paracentric inversion in human sperm. , 2007, Molecular human reproduction.

[2]  K. Mrasek,et al.  Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method , 2004, Cytogenetic and Genome Research.

[3]  T. Liehr,et al.  The DNA-based structure of human chromosome 5 in interphase. , 2002, American journal of human genetics.

[4]  R. Eils,et al.  Three-Dimensional Maps of All Chromosomes in Human Male Fibroblast Nuclei and Prometaphase Rosettes , 2005, PLoS biology.

[5]  N. Rubtsov,et al.  Reconstruction of the female Gorilla gorilla karyotype using 25-color FISH and multicolor banding (MCB) , 2001, Cytogenetic and Genome Research.

[6]  T. Liehr,et al.  Suspension (S)-FISH, a New Technique for Interphase Nuclei , 2002, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[7]  N. Carpenter,et al.  Molecular cytogenetics , 2001, Seminars in pediatric neurology.

[8]  A. Fisher,et al.  Chromosomes, positions please! , 2003, Nature Cell Biology.

[9]  T. Cremer,et al.  Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[10]  A. Pombo,et al.  Intermingling of Chromosome Territories in Interphase Suggests Role in Translocations and Transcription-Dependent Associations , 2006, PLoS biology.

[11]  T. Cremer,et al.  Chromosome territories, nuclear architecture and gene regulation in mammalian cells , 2001, Nature Reviews Genetics.

[12]  V. Trifonov,et al.  Microdissection based high resolution multicolor banding for all 24 human chromosomes. , 2002, International journal of molecular medicine.

[13]  H. Yokota,et al.  Size-dependent positioning of human chromosomes in interphase nuclei. , 2000, Biophysical journal.

[14]  T. Liehr,et al.  Evidence for interphase DNA decondensation transverse to the chromosome axis: a multicolor banding analysis. , 2002, International journal of molecular medicine.

[15]  N. Archidiacono,et al.  Comparative mapping of human alphoid sequences in great apes using fluorescence in situ hybridization. , 1995, Genomics.

[16]  V. Trifonov,et al.  Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding. , 2003, International journal of molecular medicine.

[17]  H. Tanabe,et al.  Inter- and intra-specific gene-density-correlated radial chromosome territory arrangements are conserved in Old World monkeys , 2004, Cytogenetic and Genome Research.

[18]  Peter Teague,et al.  Differences in the Localization and Morphology of Chromosomes in the Human Nucleus , 1999, The Journal of cell biology.

[19]  T. Liehr,et al.  Interphase chromosome-specific multicolor banding (ICS-MCB): a new tool for analysis of interphase chromosomes in their integrity. , 2007, Biomolecular engineering.