Performance and microbial community analysis of the anaerobic reactor with coke oven gas biomethanation and in situ biogas upgrading.

A new method for simultaneous coke oven gas (COG) biomethanation and in situ biogas upgrading in anaerobic reactor was developed in this study. The simulated coke oven gas (SCOG) (92% H2 and 8% CO) was injected directly into the anaerobic reactor treating sewage sludge through hollow fiber membrane (HFM). With pH control at 8.0, the added H2 and CO were fully consumed and no negative effects on the anaerobic degradation of sewage sludge were observed. The maximum CH4 content in the biogas was 99%. The addition of SCOG resulted in enrichment and dominance of homoacetogenetic genus Treponema and hydrogenotrophic genus Methanoculleus in the liquid, which indicated that H2 were converted to methane by both direct (hydrogenotrophic methanogenesis) and indirect (homoacetogenesis+aceticlastic methanogenesis) pathways in the liquid. However, the aceticlasitic genus Methanosaeta was dominant for archaea in the biofilm on the HFM, which indicated indirect (homoacetogenesis+aceticlastic methanogenesis) H2 conversion pathway on the biofilm.

[1]  M. Rother,et al.  Carbon monoxide-dependent energy metabolism in anaerobic bacteria and archaea , 2008, Archives of Microbiology.

[2]  Jun Shen,et al.  A New Technology for Producing Hydrogen and Adjustable Ratio Syngas from Coke Oven Gas , 2007 .

[3]  R. Moletta,et al.  Monitoring of activity dynamics of an anaerobic digester bacterial community using 16S rRNA polymerase chain reaction--single-strand conformation polymorphism analysis. , 2000, Environmental microbiology.

[4]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[5]  Irini Angelidaki,et al.  Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights , 2012, Applied Microbiology and Biotechnology.

[6]  Kj Krzysztof Ptasinski,et al.  Exergy analysis of biomass-to-synthetic natural gas (SNG) process via indirect gasification of various biomass feedstock , 2011 .

[7]  May-Britt Hägg,et al.  Techno-economic evaluation of biogas upgrading process using CO2 facilitated transport membrane , 2010 .

[8]  Irini Angelidaki,et al.  Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors , 2022 .

[9]  J. L. Navarrete-Bolaños,et al.  Numerical study of microbial population dynamics in anaerobic digestion through the Anaerobic Digestion Model No. 1 (ADM1) , 2013 .

[10]  Zhibin Yang,et al.  Steam Reforming of Coke Oven Gas for Hydrogen Production over a NiO/MgO Solid Solution Catalyst , 2010 .

[11]  Z. Hou,et al.  Production of Syngas via Partial Oxidation and CO2 Reforming of Coke Oven Gas over a Ni Catalyst , 2008 .

[12]  A. Wright,et al.  Metagenomic analysis of methanogen populations in three full-scale mesophilic anaerobic manure digesters operated on dairy farms in Vermont, USA. , 2013, Bioresource technology.

[13]  B. Rittmann,et al.  Advanced control for photoautotrophic growth and CO2-utilization efficiency using a membrane carbonation photobioreactor (MCPBR). , 2011, Environmental science & technology.

[14]  E. Pelletier,et al.  Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge , 2009, The ISME Journal.

[15]  P. He,et al.  Self-adaption of methane-producing communities to pH disturbance at different acetate concentrations by shifting pathways and population interaction. , 2013, Bioresource technology.

[16]  J. Price,et al.  Species-Specific Effects of Woody Litter on Seedling Emergence and Growth of Herbaceous Plants , 2011, PloS one.

[17]  Irini Angelidaki,et al.  Integrated biogas upgrading and hydrogen utilization in an anaerobic reactor containing enriched hydrogenotrophic methanogenic culture , 2012, Biotechnology and bioengineering.

[18]  Jean-Jacques Godon,et al.  Microbial 16S rDNA diversity in an anaerobic digester , 1997 .

[19]  A. Stams,et al.  Carbon monoxide conversion by anaerobic bioreactor sludges. , 2003, FEMS microbiology ecology.

[20]  Xionggang Lu,et al.  Performance of an oxygen-permeable membrane reactor for partial oxidation of methane in coke oven gas to syngas , 2011 .

[21]  Qi Zhou,et al.  Enhanced bioenergy recovery from rapeseed plant in a biorefinery concept. , 2011, Bioresource technology.

[22]  V. O’Flaherty,et al.  Effect of pH on growth kinetics and sulphide toxicity thresholds of a range of methanogenic, syntrophic and sulphate-reducing bacteria , 1998 .

[23]  T. Dallman,et al.  Performance comparison of benchtop high-throughput sequencing platforms , 2012, Nature Biotechnology.

[24]  Serge R. Guiot,et al.  Potential of wastewater-treating anaerobic granules for biomethanation of synthesis gas. , 2011, Environmental science & technology.

[25]  Alexander Goesmann,et al.  Comparative and Joint Analysis of Two Metagenomic Datasets from a Biogas Fermenter Obtained by 454-Pyrosequencing , 2011, PloS one.

[26]  Yongfa Zhang,et al.  CO2 reforming of CH4 in coke oven gas to syngas over coal char catalyst , 2010 .

[27]  S. Okabe,et al.  Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge , 2011, The ISME Journal.

[28]  S. Kleinsteuber,et al.  Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials , 2013, Applied Microbiology and Biotechnology.

[29]  Prathap Parameswaran,et al.  Focused-Pulsed sludge pre-treatment increases the bacterial diversity and relative abundance of acetoclastic methanogens in a full-scale anaerobic digester. , 2009, Water research.