Pituitary proopiomelanocortin-derived peptides and hypothalamus-pituitary-interrenal axis activity in gilthead sea bream (Sparus aurata) during prolonged crowding stress: differential regulation of adrenocorticotropin hormone and alpha-melanocyte-stimulating hormone release by corticotropin-releasin

Plasma levels of cortisol, growth hormone (GH), adrenocorticotropin hormone (ACTH), alpha-melanocyte-stimulating hormone (alpha-MSH), N-acetyl-beta-endorphin, in vitro ACTH-stimulated cortisol secretion, and in vitro corticotropin-releasing hormone (CRH)- and thyrotropin-releasing hormone (TRH)-stimulated ACTH and alpha-MSH secretion were investigated in gilthead sea bream exposed to high stocking density (30 kg m(-3)) for 23 days. Within 3 days after the onset of crowding, plasma levels of cortisol, ACTH, alpha-MSH, and N-acetyl-beta-endorphin were above control values. After 7 days, plasma parameters had returned to control levels, but at 23 days, cortisol, alpha-MSH, and N-acetyl-beta-endorphin levels were again elevated over controls, indicating a long-term activation of the melanotrope cells. In contrast, crowding stress elicited a prolonged reduction in plasma GH levels concomitant with the increased hypothalamus-pituitary-interrenal axis (HPI) activation. Crowding stress enhanced cortisol secretory activity of the unstimulated interrenal cells. However, interrenal tissue from crowded fish in vitro displayed an attenuated response to ACTH stimulation compared with tissue from control fish, indicating a desensitization of these cells to ACTH during crowding. The involvement of pituitary proopiomelanocortin-derived peptides in the HPI axis of sea bream is indicated by the observed modulation of the CRH and TRH responsiveness of the corticotropes and melanotropes in crowded fish. At day 1, when there were crowding-induced plasma increases in ACTH and alpha-MSH, there was an attenuated CRH-stimulated but not TRH-stimulated, ACTH release. However, at that time, CRH- and TRH-induced responses of alpha-MSH secretion, and the unstimulated secretory activity of the MSH cells, were enhanced in crowded sea bream. These data provide evidence for stimulatory roles of multiple hypothalamic (CRH and TRH) and pituitary (ACTH and alpha-MSH) peptides in the activation of the hypothalamus-pituitary-interrenal axis under crowding conditions in sea bream.

[1]  G. Flik,et al.  The stress response of the gilthead sea bream (Sparus aurata L.) to air exposure and confinement. , 1999, The Journal of endocrinology.

[2]  J. Pérez‐Sánchez,et al.  Growth hormone axis as marker of nutritional status and growth performance in fish , 1999 .

[3]  M. Izquierdo,et al.  Effect of vitamin E and C dietary supplementation on some immune parameters of gilthead seabream (Sparus aurata) juveniles subjected to crowding stress , 1999 .

[4]  F. Facchinetti,et al.  Acetyl Salmon Endorphin-Like and Interrenal Stress Response in Male Gilthead Sea Bream, Sparus aurata , 1998, Neuroendocrinology.

[5]  G. Iwama.  Stress in Fish , 1998 .

[6]  M. Pavlidis,et al.  Non-specific immune responses in the red porgy Pagrus pagrus after crowding stress , 1997 .

[7]  Tort,et al.  Cortisol and glucose responses after acute stress by net handling in the sparid red porgy previously subjected to crowding stress , 1997, Journal of fish biology.

[8]  G. Iwama,et al.  Metabolic Responses Associated with Confinement Stress in Tilapia: The Role of Cortisol , 1997 .

[9]  L. Tort,et al.  Crowding stress induces changes in serum haemolytic and agglutinating activity in the gilthead sea bream Sparus aurata. , 1996, Veterinary immunology and immunopathology.

[10]  S. W. Bonga,et al.  Endorphin and MSH in concert form the corticotropic principle released by tilapia (Oreochromis mossambicus; Teleostei) melanotropes , 1995, Peptides.

[11]  P. Balm,et al.  Corticotrope and melanotrope POMC-derived peptides in relation to interrenal function during stress in rainbow trout (Oncorhynchus mykiss). , 1995, General and comparative endocrinology.

[12]  L. Tort,et al.  Effects of daily management stress on haematology and blood rheology of the gilthead seabream , 1995 .

[13]  J. Pérez‐Sánchez,et al.  The use of recombinant gilthead sea bream (Sparus aurata) growth hormone for radioiodination and standard preparation in radioimmunoassay. , 1995, Comparative biochemistry and physiology. Part A, Physiology.

[14]  P. Balm,et al.  Adrenocorticotropic hormone in relation to interrenal function during stress in tilapia (Oreochromis mossambicus). , 1994, General and comparative endocrinology.

[15]  G. Flik,et al.  A specific role for TRH in release of diacetyl alpha-MSH in tilapia stressed by acid water. , 1994, The American journal of physiology.

[16]  R. Dores,et al.  Proteolytic cleavage of ACTH in corticotropes of sexually mature axolotls (Ambystoma mexicanum) , 1993, Peptides.

[17]  G. Wand,et al.  Stress alters adenylyl cyclase activity in the pituitary and frontal cortex of the rat. , 1993, Life sciences.

[18]  G. Flik,et al.  A role for di-acetyl alpha-melanocyte-stimulating hormone in the control of cortisol release in the teleost Oreochromis mossambicus. , 1992, The Journal of endocrinology.

[19]  A. Giustina,et al.  The role of glucocorticoids in the regulation of growth hormone secretion Mechanisms and clinical significance , 1992, Trends in Endocrinology & Metabolism.

[20]  C. Schreck,et al.  Evidence for ultra-short-loop feedback in ACTH-induced interrenal steroidogenesis in coho salmon: acute self-suppression of cortisol secretion in vitro. , 1992, General and comparative endocrinology.

[21]  J. Sumpter,et al.  Effects of acute and chronic stress on the levels of circulating growth hormone in the rainbow trout, Oncorhynchus mykiss. , 1991, General and comparative endocrinology.

[22]  R. Dores,et al.  Differential mechanisms for the N-acetylation of alpha-melanocyte-stimulating hormone and beta-endorphin in the intermediate pituitary of the frog, Xenopus laevis. , 1991, Neuroendocrinology.

[23]  M. Vijayan,et al.  High stocking density affects cortisol secretion and tissue distribution in brook charr, Salvelinus fontinalis. , 1990, The Journal of endocrinology.

[24]  B. Jenks,et al.  Dynamics of background adaptation in Xenopus laevis: role of catecholamines and melanophore-stimulating hormone. , 1989, General and comparative endocrinology.

[25]  B. Gillham,et al.  Glucocorticoids act rapidly in vitro to attenuate second messenger responses to ACTH secretagogues in rats. , 1989, The Journal of endocrinology.

[26]  H. Vaudry,et al.  TRH stimulates the release of POMC-derived peptides from goldfish melanotropes , 1989, Peptides.

[27]  R. J. Omeljaniuk,et al.  Dopamine inhibition of gonadotropin and alpha-melanocyte-stimulating hormone release in vitro from the pituitary of the goldfish (Carassius auratus). , 1989, General and comparative endocrinology.

[28]  G. Young.  Enhanced response of the interrenal of coho salmon (Oncorhynchus kisutch) to ACTH after growth hormone treatment in vivo and in vitro. , 1988, General and comparative endocrinology.

[29]  R. Tripp,et al.  Cellular mechanisms of glucocorticoid immunosuppression in salmon , 1987 .

[30]  J. Rivier,et al.  Inhibition of CRF- and urotensin I-stimulated ACTH release from goldfish pituitary cell columns by the CRF analogue α-helical CRF-(9–41) , 1987, Regulatory Peptides.

[31]  A. D. Pickering,et al.  Crowding causes prolonged leucopenia in salmonid fish, despite interrenal acclimation , 1987 .

[32]  C. Schreck,et al.  Changes in the Immune System of Coho Salmon (Oncorhynchus kisutch) during the Parr-to-Smolt Transformation and after Implantation of Cortisol , 1987 .

[33]  J. Fryer,et al.  Control of Corticotropin Secretion in Teleost Fishes , 1986 .

[34]  C. Schreck,et al.  Effects of rearing conditions on the developmental physiology of smolting coho salmon , 1986 .

[35]  H. M. Dye,et al.  The effects of stress on plasma ACTH, α-MSH, and cortisol levels in salmonid fishes , 1986 .

[36]  A. D. Pickering,et al.  Acclimation of the interrenal tissue of the brown trout, Salmo trutta L., to chronic crowding stress , 1984 .

[37]  A. F. Cook,et al.  The effects of somatostatin on serum growth hormone levels in the goldfish, Carassius auratus. , 1984, General and comparative endocrinology.

[38]  H. Kawauchi,et al.  Chemical and immunological characterization of salmon endorphins. , 1984, General and comparative endocrinology.

[39]  A. D. Pickering.  Cortisol-induced lymphocytopenia in brown trout, Salmo trutta L. , 1984, General and comparative endocrinology.

[40]  E. Donaldson.  pituitary-interrenal axis as an indicator of stress in fish , 1981 .