Some potentialities and drawbacks of contemporary size-exclusion chromatography.

The present state of the chromatographic techniques based on differentiation of solutes according to their molecular sizes is briefly surveyed. Attention is centred on high-performance techniques applied to purification and characterization of natural macromolecules, and on discussion of the chromatographic approaches to the determination of the molecular masses and molecular mass distributions of both natural and synthetic polymers. The basic requirements on the selection of the separation system and the experimental conditions are summarized, demonstrated on a few examples and critically evaluated.

[1]  W. Mcclennen,et al.  Fractionation and characterization of phenolic resins by high-performance liquid chromatography and gel-permeation chromatography combined with ultraviolet, refractive index, mass spectrometry and light-scattering detection. , 2001, Journal of chromatography. A.

[2]  R. Fitzgerald,et al.  Characterisation of a new chromatography matrix for peptide molecular mass determination , 1997 .

[3]  N. Hilschmann,et al.  High Performance Liquid Chromatography of Peptides and Proteins , 1983 .

[4]  P. Dubin,et al.  Aqueous size exclusion chromatography , 1989 .

[5]  G. Dollinger,et al.  Practical on-line determination of biopolymer molecular weights by high-performance liquid chromatography with classical light-scattering detection , 1992 .

[6]  D. Prazeres.  A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography , 1997 .

[7]  S. Lustig.  Peak-referenced integral method for size exclusion chromatography and its application to aromatic polyesters , 1999 .

[8]  R. Kandiyoti,et al.  Absolute calibration of size exclusion chromatography for coal derivatives through MALDI-m.s. , 1998 .

[9]  J. Smit,et al.  Size-exclusion chromatography--multiangle laser light scattering analysis of beta-lactoglobulin and bovine serum albumin in aqueous solution with added salt. , 2000, Journal of chromatography. A.

[10]  I. Krull,et al.  Characterization of antigen-antibody complexes by size-exclusion chromatography coupled with low-angle light-scattering photometry and viscometry. , 1997, Journal of chromatography. A.

[11]  I. Krull,et al.  Determination of pituitary and recombinant human growth hormone molecular weights by modern high-performance liquid chromatography with low angle laser light scattering detection. , 1991, Journal of chromatography.

[12]  R D Ricker,et al.  Fast, reproducible size-exclusion chromatography of biological macromolecules. , 1996, Journal of chromatography. A.

[13]  A. Revillon.  Alternatives to Size Exclusion Chromatography , 1994 .

[14]  M. Nielen.  Characterization of Synthetic Polymers by Size‐exclusion Chromatography/Electrospray Ionization Mass Spectrometry , 1996 .

[15]  S. Mori,et al.  Effect of column temperatures on molecular weight determination by high performance size exclusion chromatography , 1980 .

[16]  L. Fischer.  Gel Filtration Chromatography , 1980, Definitions.

[17]  E. W. Meijer,et al.  Off-line size-exclusion chromatographic fractionation-matrix-assisted laser desorption ionization time-of-flight mass spectrometry for polymer characterization. Theoretical and experimental study. , 2000, Journal of chromatography. A.

[18]  K. Stulík,et al.  High-performance separations in isolation and characterization of allergens. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[19]  M. Montaudo,et al.  Application of size exclusion chromatography matrix-assisted laser desorption/ionization time-of-flight to the determination of molecular masses in polydisperse polymers , 1998 .

[20]  K. Stulík,et al.  Comparison of high-performance liquid chromatography and capillary electrophoresis for the determination of some bee venom components , 1995 .

[21]  J. J. Kirkland,et al.  Errors caused by flowrate variation in high performance size exclusion chromatography (GPC) [gel permeation chromatography] , 1975 .

[22]  G. Hohner,et al.  Characterization of technical waxes comparison of chromatographic techniques and matrix-assisted laser-desorption/ionization mass spectrometry , 1996 .

[23]  Chi Wu.  Light-scattering Characterization of the Molecular Weight Distributions of Some Intractable Polymers , 1997 .

[24]  H G Barth,et al.  Size exclusion chromatography. , 1992, Analytical chemistry.

[25]  J. Jorgenson,et al.  Comprehensive three-dimensional separation of peptides using size exclusion chromatography/reversed phase liquid chromatography/optically gated capillary zone electrophoresis. , 1995, Analytical chemistry.

[26]  H. Komatsu,et al.  Molecular mass determination of low-molecular-mass heparins. Application of wide collection angle measurements of light scattering using a high-performance gel permeation chromatographic system equipped with a low-angle laser light-scattering photometer. , 1993, Journal of chromatography.

[27]  R. Kandiyoti,et al.  N-methyl-2-pyrrolidinone as a mobile phase in the size-exclusion chromatography of coal derivatives , 1997 .

[28]  M. Netopilík.  Influence of peak-broadening and interdetector volume error on size-exclusive chromatographic analysis with dual viscometric-concentration detection using the universal calibration method. , 2001, Journal of chromatography. A.

[29]  R. J. White.  FFF–MALS—A New Tool for the Characterisation of Polymers and Particles , 1997 .