Remodeling of an acellular collagen graft into a physiologically responsive neovessel

Surgical treatment of vascular disease has become common, creating the need for a readily available, small-diameter vascular graft. However, the use of synthetic materials is limited to grafts larger than 5–6 mm because of the frequency of occlusion observed with smaller-diameter prosthetics. An alternative to synthetic materials would be a biomaterial that could be used in the design of a tissue-engineered graft. We demonstrate that a small-diameter (4 mm) graft constructed from a collagen biomaterial derived from the submucosa of the small intestine and type I bovine collagen has the potential to integrate into the host tissue and provide a scaffold for remodeling into a functional blood vessel. The results obtained using a rabbit arterial bypass model have shown excellent hemostasis and patency. Furthermore, within three months after implantation, the collagen grafts were remodeled into cellularized vessels that exhibited physiological activity in response to vasoactive agents.

[1]  R. Moore,et al.  Reduction of the surgical complication rate by the use of a hypothrombogenic catheter coating. , 1973, Radiology.

[2]  E Bell,et al.  A blood vessel model constructed from collagen and cultured vascular cells. , 1986, Science.

[3]  R. Nerem,et al.  Co-Culture of Endothelial Cells and Smooth Muscle Cells in a Flow Environment: An Improved Culture Model of the Vascular Wall? , 1995 .

[4]  T. O'donnell,et al.  Correlation of operative findings with angiographic and noninvasive hemodynamic factors associated with failure of polytetrafluoroethylene grafts. , 1984, Journal of vascular surgery.

[5]  J. Anderson,et al.  Effect of albumin coating on the in vitro blood compatibility of Dacron arterial prostheses. , 1989, Biomaterials.

[6]  W. F. Holdefer,et al.  The importance of proper caliber of lumen in femoral-popliteal artery reconstruction. , 1966, Surgery, gynecology & obstetrics.

[7]  F A Auger,et al.  A completely biological tissue‐engineered human blood vessel , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[8]  T. Okano,et al.  Heparin immobilization onto segmented polyurethane-urea surfaces--effect of hydrophilic spacers. , 1988, Journal of biomedical materials research.

[9]  R Langer,et al.  Functional arteries grown in vitro. , 1999, Science.

[10]  G. Herrera,et al.  Role of glutaraldehyde in calcification of porcine aortic valve fibroblasts. , 1999, The American journal of pathology.

[11]  G. Downing,et al.  Modification of native collagen reduces antigenicity but preserves cell compatibility , 2000, Biotechnology and bioengineering.

[12]  C. Heisterkamp,et al.  The fate of the inverted segment of small bowel used for the replacement of major veins. , 1966, Surgery.

[13]  L A Geddes,et al.  Small intestinal submucosa as a large diameter vascular graft in the dog. , 1989, The Journal of surgical research.

[14]  J. Guilhou,et al.  [Rapid healing of venous ulcers and lack of clinical rejection with an allogeneic cultured human skin equivalent]. , 1999, Annales de dermatologie et de venereologie.

[15]  T. Mihaljevic,et al.  Superior late patency of small-diameter Dacron grafts seeded with omental microvascular cells: an experimental study. , 1994, The Annals of thoracic surgery.

[16]  P. Hagen,et al.  Increased vascular contraction and sensitivity to norepinephrine after endothelial denudation is inhibited by prazosin. , 1986, Surgery.

[17]  T. Matsuda,et al.  A hybrid vascular model biomimicking the hierarchic structure of arterial wall: neointimal stability and neoarterial regeneration process under arterial circulation. , 1995, The Journal of thoracic and cardiovascular surgery.

[18]  R T Tranquillo,et al.  Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated media-equivalent. , 1996, Biomaterials.

[19]  M. Davies,et al.  The expression and function of G-proteins in experimental intimal hyperplasia. , 1994, The Journal of clinical investigation.

[20]  S. Williams,et al.  Endothelial cell transplantation onto polymeric arteriovenous grafts evaluated using a canine model. , 1994, Journal of investigative surgery : the official journal of the Academy of Surgical Research.

[21]  G. Bowlin,et al.  Electrostatic Endothelial Cell Seeding Technique for Small-Diameter (<6 MM) Vascular Prostheses: Feasibility Testing , 1997 .

[22]  J. Glover,et al.  A single-staged technique for seeding vascular grafts with autogenous endothelium. , 1978, Surgery.

[23]  P. Davies,et al.  Flow-mediated endothelial mechanotransduction. , 1995, Physiological reviews.

[24]  S. Gundry,et al.  A quantitative and qualitative comparison of fibrin glue, albumin, and blood as agents to pretreat porous vascular grafts. , 1987, The Journal of surgical research.

[25]  J. Stanley.  Biologic and synthetic vascular prostheses , 1982 .

[26]  S. Egusa.  Experimental study on vascular graft. II. Replacement of inferior vena cava and abdoninal aorta with the autogenous segment of small intestine submucosa. , 1968, Acta medicinae Okayama.

[27]  C. Giachelli,et al.  Ectopic calcification: gathering hard facts about soft tissue mineralization. , 1999, The American journal of pathology.

[28]  J. Foster,et al.  Evaluation of canine intestinal submucosa as a vascular substitute. , 1971, American journal of surgery.

[29]  R M Nerem,et al.  The study of rheological effects on vascular endothelial cells in culture. , 1989, Biorheology.

[30]  L A Geddes,et al.  Small intestinal submucosa as a small-diameter arterial graft in the dog. , 1990, Journal of investigative surgery : the official journal of the Academy of Surgical Research.