Label-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor.

We have developed a low-cost optical cavity-based biosensor with a differential detection method for point-of-care medical diagnostics. To experimentally demonstrate its label-free real-time biosensing capability, we performed the detection of biotinylated bovine serum albumin (BSA). Streptavidin is introduced into the optical cavity structure and immobilized on 3-aminopropyltriethoxysilane (APTES) coated surface. After rinsing out unbound streptavidin with DI water, biotinylated BSA without any labeling is introduced. A CMOS camera captures the transmitted light of two different wavelengths passing through the optical cavity sensing area in real-time. Then, the differential values are calculated to enhance the responsivity. We successfully demonstrated the label-free real-time detection of biotinylated BSA, and the measurement results matched well with the simulation results. The limit of detection of the optical cavity-based biosensor for the biotinylated BSA detection with the sensing area of 180 μm × 180 μm is estimated to be 2.82 pM, which could be reduced further for a smaller sensing area with the tradeoff of a longer sensing time.

[1]  Seokheun Choi,et al.  Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins , 2010, Microfluidics and nanofluidics.

[2]  Xudong Fan,et al.  On the performance quantification of resonant refractive index sensors. , 2008, Optics express.

[3]  Mischa Megens,et al.  Integrated microfluidic bioprocessor for solid phase capture immunoassays. , 2011, Lab on a chip.

[4]  Kai Sun,et al.  Field-effect sensors - from pH sensing to biosensing: sensitivity enhancement using streptavidin-biotin as a model system. , 2017, The Analyst.

[5]  Joseph Wang,et al.  Point-of-care biosensor systems for cancer diagnostics/prognostics. , 2006, Biosensors & bioelectronics.

[6]  A. Majumdar,et al.  Stamp-and-stick room-temperature bonding technique for microdevices , 2005, Journal of Microelectromechanical Systems.

[7]  D Rho,et al.  Low-cost optical cavity based sensor with a large dynamic range. , 2017, Optics express.

[8]  W. Bishai,et al.  Diagnostic point-of-care tests in resource-limited settings. , 2014, The Lancet. Infectious diseases.

[9]  Donggee Rho,et al.  Large dynamic range optical cavity based sensor using a low cost three-laser system , 2017, 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC).

[10]  S. Gan,et al.  Enzyme immunoassay and enzyme-linked immunosorbent assay. , 2013, The Journal of investigative dermatology.

[11]  J. H. Zhang,et al.  The origin of the cooperativity in the streptavidin-biotin system: A computational investigation through molecular dynamics simulations , 2016, Scientific Reports.

[12]  Emanuel Carrilho,et al.  Paper-based ELISA. , 2010, Angewandte Chemie.

[13]  Fatimah Ibrahim,et al.  A Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) Detection Platform for a Point-of-Care Dengue Detection System on a Lab-on-Compact-Disc , 2015, Sensors.

[14]  Joshua Brake,et al.  Refractive index measurement using an optical cavity based biosensor with a differential detection , 2015, Photonics West - Biomedical Optics.

[15]  Peter Cowles,et al.  Preliminary measurement results of biotinylated BSA detection of a low cost optical cavity based biosensor using differential detection , 2016, SPIE BiOS.