Selected memory T cells infused post–haploidentical hematopoietic stem cell transplantation persist and hyperexpand

Key Points • Infusions of donor memory T-cells after haplo-HSCT lead to engraftment, persistence, and prominent expansion of selected T-cell clones.• Infused patients harbor highly functional CMV-specific T-cells and their expansion correlates with their frequency in the donor.

[1]  M. Maschan,et al.  Results of Hematopoietic Stem Cells Transplantation with Tcrαβ and CD19-Depletion from Matched Related Donors and Infusions of CD45RA Depleted Donor Lymphocytes in Pediatric Severe Aplastic Anemia , 2021, Blood.

[2]  A. Santoro,et al.  Single-cell profiling reveals the dynamics of cytomegalovirus-specific T cells in haploidentical hematopoietic stem cell transplantation , 2021, Haematologica.

[3]  C. Peano,et al.  Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT , 2021, JCI insight.

[4]  L. Giordano,et al.  Feasibility and Efficacy of CD45RA+ Depleted Donor Lymphocytes Infusion After Haploidentical Transplantation With Post-Transplantation Cyclophosphamide in Patients With Hematological Malignancies. , 2021, Transplantation and cellular therapy.

[5]  M. Maschan,et al.  Safety and efficacy of the low-dose memory (CD45RA-depleted) donor lymphocyte infusion in recipients of αβ T cell-depleted haploidentical grafts: results of a prospective randomized trial in high-risk childhood leukemia , 2021, Bone Marrow Transplantation.

[6]  Shannon K. Boi,et al.  Stem, Effector, and Hybrid States of Memory CD8+ T Cells. , 2019, Trends in immunology.

[7]  B. Becher,et al.  Development, application and computational analysis of high-dimensional fluorescent antibody panels for single-cell flow cytometry , 2019, Nature Protocols.

[8]  D. Venzon,et al.  Post-transplantation cyclophosphamide prevents graft-versus-host disease by inducing alloreactive T cell dysfunction and suppression. , 2019, The Journal of clinical investigation.

[9]  Theresa Kaeuferle,et al.  Strategies of adoptive T -cell transfer to treat refractory viral infections post allogeneic stem cell transplantation , 2019, Journal of Hematology & Oncology.

[10]  Y. Li,et al.  Selective T‐cell depletion targeting CD45RA reduces viremia and enhances early T‐cell recovery compared with CD3‐targeted T‐cell depletion , 2018, Transplant infectious disease : an official journal of the Transplantation Society.

[11]  N. Restifo,et al.  Epigenetic control of CD8+ T cell differentiation , 2018, Nature Reviews Immunology.

[12]  B. Storer,et al.  Origin and evolution of the T cell repertoire after posttransplantation cyclophosphamide. , 2016, JCI insight.

[13]  L. Luznik,et al.  Modern approaches to HLA-haploidentical blood or marrow transplantation. , 2016, Nature Reviews Clinical Oncology.

[14]  Mikhail Pogorelyy,et al.  VDJtools: Unifying Post-analysis of T Cell Receptor Repertoires , 2015, PLoS Comput. Biol..

[15]  Sean C. Bendall,et al.  Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis , 2015, Cell.

[16]  Bruce R. Blazar,et al.  CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCRβ repertoire. , 2015, Blood.

[17]  A. Santoro,et al.  Role of naive-derived T memory stem cells in T-cell reconstitution following allogeneic transplantation. , 2015, Blood.

[18]  S. Bicciato,et al.  Generation of human memory stem T cells after haploidentical T-replete hematopoietic stem cell transplantation. , 2015, Blood.

[19]  Mikhail Shugay,et al.  MiXCR: software for comprehensive adaptive immunity profiling , 2015, Nature Methods.

[20]  C. Carlo-Stella,et al.  Infections after T‐replete haploidentical transplantation and high‐dose cyclophosphamide as graft‐versus‐host disease prophylaxis , 2015, Transplant infectious disease : an official journal of the Transplantation Society.

[21]  N. Hacohen,et al.  Reversal of in situ T-cell exhaustion during effective human antileukemia responses to donor lymphocyte infusion. , 2014, Blood.

[22]  K. Komanduri,et al.  Antigen and lymphopenia-driven donor T cells are differentially diminished by post-transplantation administration of cyclophosphamide after hematopoietic cell transplantation. , 2013, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[23]  Dmitriy A Bolotin,et al.  Quantitative tracking of T cell clones after haematopoietic stem cell transplantation , 2011, EMBO molecular medicine.

[24]  Mario Roederer,et al.  SPICE: Exploration and analysis of post‐cytometric complex multivariate datasets , 2011, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[25]  Allen R. Chen,et al.  HLA-haploidentical bone marrow transplantation for hematologic malignancies using nonmyeloablative conditioning and high-dose, posttransplantation cyclophosphamide. , 2008, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[26]  J. Whitesides,et al.  Inability of memory T cells to induce graft-versus-host disease is a result of an abortive alloresponse. , 2007, Blood.

[27]  B. Levine,et al.  Dendritic cell-activated CD44hiCD8+ T cells are defective in mediating acute graft-versus-host disease but retain graft-versus-leukemia activity. , 2004, Blood.

[28]  N. Chao,et al.  Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease. , 2004, Blood.

[29]  M. Shlomchik,et al.  Memory CD4+ T cells do not induce graft-versus-host disease. , 2003, The Journal of clinical investigation.

[30]  P. Moss,et al.  Cytomegalovirus Seropositivity Drives the CD8 T Cell Repertoire Toward Greater Clonality in Healthy Elderly Individuals1 , 2002, The Journal of Immunology.

[31]  M. Leffell,et al.  Nonmyeloablative bone marrow transplantation from partially HLA-mismatched related donors using posttransplantation cyclophosphamide. , 2002, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[32]  C. Mackall,et al.  Thymic-independent T cell regeneration occurs via antigen-driven expansion of peripheral T cells resulting in a repertoire that is limited in diversity and prone to skewing. , 1996, Journal of immunology.

[33]  Elizabeth Brunk,et al.  Antigen receptor repertoire profiling from RNA-seq data , 2022 .