Chitosan Scaffold for Wound Healing Application

-Chitosan is natural polysaccharide resembles with glycosaminoglycan of Extra Cellular matrix (ECM), which makes it a promising candidate for wound healing application apart from its excellent biocompatibility and biodegradability. In this study we have formulated various types of chitosan gel by dissolving different concentrations of chitosan powder in 100 ml of 2% acetic acid separately. Electrolytes were also added during stirring which was carried out for 5 ±0.5h at 50 0 C. Different types of chitosan gel thus obtained were lyophilized for 48 h to remove water and chitosan scaffold were obtained and characterized using Scanning electron microscopy (SEM), FT-IR and swelling test. FT-IR confirms the chemical identity of chitosan in scaffolds. SEM micrographs of polymeric hydrogel scaffolds synthesized using 2% of chitosan was found to be the best in various physiochemical properties. The swelling capacity of 2% Chitosan scaffold was found to be better in comparison to rest of the scaffolds. Keywords— Chitosan, Histopathology, Wound Healing, Scaffold.