Synthesis and Characterization of Dye-Labeled Poly(methacrylic acid) Grafted Silica Nanoparticles.

The synthesis of dye-labeled poly(methacrylic acid) (PMAA) grafted silica nanoparticles was studied. Surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization of tert-butylmethacrylate (tBuMA) was conducted on dye-labeled CPDB coated silica nanoparticles followed by sequential removal of the thiocarbonylthio end groups and the tert-butyl moieties. Additionally, as a more straightforward strategy, direct polymerization of methacrylic acid on silica nanoparticles with a diameter size as small as 15 nm was conducted via the RAFT polymerization technique. A variety of PMAA brushes with different lengths and densities were prepared on nanoparticle surfaces via surface-initiated RAFT polymerization with excellent control and surface grafting densities as high as 0.65 chains/nm2. The grafted PMAA was methylated by trimethylsilyldiazomethane to conduct organic phase GPC characterization. The dye-labeled PMAA grafted nanoparticles provide a platform to bind biomolecules and to track the movement of the nanoparticles in biological systems.

[1]  Lei Wang,et al.  The preparation and characterization of carboxylic acid-coated silica nanoparticles , 2012 .

[2]  Richey M. Davis,et al.  Magnetic Block Ionomer Complexes for Potential Dual Imaging and Therapeutic Agents , 2012 .

[3]  Yoshinobu Nakamura,et al.  pH-responsive disruption of ‘liquid marbles’ prepared from water and poly(6-(acrylamido) hexanoic acid)-grafted silica particles , 2011 .

[4]  X. Qu,et al.  Polyvalent nucleic acid/mesoporous silica nanoparticle conjugates: dual stimuli-responsive vehicles for intracellular drug delivery. , 2011, Angewandte Chemie.

[5]  Tao Wu,et al.  Fabrication of Photoswitchable and Thermotunable Multicolor Fluorescent Hybrid Silica Nanoparticles Coated with Dye-Labeled Poly(N-isopropylacrylamide) Brushes , 2009 .

[6]  Linda S. Schadler,et al.  Anisotropic self-assembly of spherical polymer-grafted nanoparticles. , 2009, Nature materials.

[7]  Vincent M. Rotello,et al.  Applications of Nanoparticles in Biology , 2008 .

[8]  H. Zou,et al.  Polymer/silica nanocomposites: preparation, characterization, properties, and applications. , 2008, Chemical reviews.

[9]  J. Genzer,et al.  Behavior of Surface-Anchored Poly(acrylic acid) Brushes with Grafting Density Gradients on Solid Substrates: 1. Experiment , 2007 .

[10]  P. Charpentier,et al.  Synthesis of TiO2/PAA nanocomposite by RAFT polymerization , 2007 .

[11]  Weihong Tan,et al.  FloDots: luminescent nanoparticles , 2006, Analytical and bioanalytical chemistry.

[12]  C. Ryu,et al.  A Versatile Method To Prepare RAFT Agent Anchored Substrates and the Preparation of PMMA Grafted Nanoparticles , 2006 .

[13]  N. Ayres,et al.  A Facile Route to Poly(acrylic Acid) Brushes Using Atom Transfer Radical Polymerization , 2006 .

[14]  Wilhelm T S Huck,et al.  Locking and unlocking of polyelectrolyte brushes: toward the fabrication of chemically controlled nanoactuators. , 2005, Angewandte Chemie.

[15]  Graeme Moad,et al.  Living radical polymerization by the RAFT process , 2005 .

[16]  B. Benicewicz,et al.  Synthesis of well-defined polymer brushes grafted onto silica nanoparticles via surface reversible addition-fragmentation chain transfer polymerization , 2005 .

[17]  Y. Hsieh,et al.  Enzyme immobilization on ultrafine cellulose fibers via poly(acrylic acid) electrolyte grafts. , 2005, Biotechnology and bioengineering.

[18]  Bin Zhao,et al.  Environmentally responsive "hairy" nanoparticles: mixed homopolymer brushes on silica nanoparticles synthesized by living radical polymerization techniques. , 2005, Journal of the American Chemical Society.

[19]  K. Johnsson,et al.  Protein-functionalized polymer brushes. , 2005, Biomacromolecules.

[20]  D. Bhattacharyya,et al.  Separation of dilute electrolytes in poly(amino acid) functionalized microporous membranes: model evaluation and experimental results , 2004 .

[21]  Kemin Wang,et al.  Luminescent nanoparticle probes for bioimaging. , 2004, Journal of nanoscience and nanotechnology.

[22]  J. Chiefari,et al.  Living free-radical polymerization by reversible addition - Fragmentation chain transfer: The RAFT process , 1998 .