Anti-Obesity and Anti-Inflammatory Synergistic Effects of Green Tea Catechins and Citrus β-Cryptoxanthin Ingestion in Obese Mice

Chronic obesity causes various diseases, leading to an urgent need for its treatment and prevention. Using monosodium-glutamate-induced obesity mice, the present study investigated the synergistic obesity-reducing effects of tea catechins and the antioxidant β-cryptoxanthin present in mandarin oranges. The results show that the obese mice that ingested both tea catechin and β-cryptoxanthin for 4 weeks had a significantly decreased body weight, with no difference in body weight compared with control mice. Moreover, the blood biochemical test results were normal, and the body fat percentage was significantly decreased according to the histopathological analysis. Additionally, the abundance of M1 macrophages, which release pro-inflammatories, was significantly reduced in adipose tissue. Indeed, a significant decrease was detected in M1-macrophage-secreted tumor necrosis factor-alpha levels. Meanwhile, M2 macrophage levels were recovered, and adiponectin, which is released from adipocytes and involved in suppressing metabolic syndrome, was increased. Collectively, these results suggest that the combination of tea catechins and antioxidant foods can alleviate chronic obesity, indicating that a combination of various ingredients in foods might contribute to reducing chronic obesity.

[1]  R. González-Domínguez,et al.  Altered Metal Homeostasis Associates with Inflammation, Oxidative Stress, Impaired Glucose Metabolism, and Dyslipidemia in the Crosstalk between Childhood Obesity and Insulin Resistance , 2022, Antioxidants.

[2]  A. Feraco,et al.  Nutraceuticals in Brown Adipose Tissue Activation , 2022, Cells.

[3]  D. Granato,et al.  Clitoria ternatea blue petal extract protects against obesity, oxidative stress, and inflammation induced by a high-fat, high-fructose diet in C57BL/6 mice. , 2022, Food research international.

[4]  Haohong Li,et al.  Chronic high-fat diet induces overeating and impairs synaptic transmission in feeding-related brain regions , 2022, Frontiers in Molecular Neuroscience.

[5]  O. Porzio,et al.  Study of the Association between Thiols and Oxidative Stress Markers in Children with Obesity , 2022, Nutrients.

[6]  Varnavas D. Mouchlis,et al.  Vitamin E Functions by Association with a Novel Binding Site on the 67 kDa Laminin Receptor Activating Diacylglycerol Kinase. , 2022, The Journal of nutritional biochemistry.

[7]  María Pineros-Leano,et al.  Childhood obesity risk factors by race and ethnicity , 2022, Obesity.

[8]  Ximing Huang,et al.  Intermolecular hydrogen bonds between catechin and theanine in tea: slow release of the antioxidant capacity by a synergetic effect , 2022, RSC advances.

[9]  R. Bruno,et al.  EGCG and Catechin Relative to Green Tea Extract Differentially Modulate the Gut Microbial Metabolome and Liver Metabolome To Prevent Obesity In Mice Fed A High-Fat Diet. , 2022, The Journal of nutritional biochemistry.

[10]  S. Santosa,et al.  Adipocyte Size, Adipose Tissue Fibrosis, Macrophage Infiltration and Disease Risk Are Different in Younger and Older Individuals with Childhood and Adulthood Onset Obesity , 2022, International Journal of Obesity.

[11]  K. Nakadate,et al.  Effects of Smart Drugs on Cholinergic System and Non-Neuronal Acetylcholine in the Mouse Hippocampus: Histopathological Approach , 2022, Journal of clinical medicine.

[12]  Soo-Young Lee,et al.  Anti-Obesity and Anti-Adipogenic Effects of Administration of Arginyl-Fructose-Enriched Jeju Barley (Hordeum vulgare L.) Extract in C57BL/6 Mice and in 3T3-L1 Preadipocytes Models , 2022, Molecules.

[13]  Mark J. Ramos,et al.  Use of Real‐World Data and Physiologically‐Based Pharmacokinetic Modeling to Characterize Enoxaparin Disposition in Children With Obesity , 2022, Clinical pharmacology and therapeutics.

[14]  J. Buckley,et al.  In-utero co-exposure to toxic metals and micronutrients on childhood risk of overweight or obesity: New insight on micronutrients counteracting toxic metals , 2022, International Journal of Obesity.

[15]  P. Roger,et al.  Antioxidant and Anticholinesterase Properties of the Aqueous Extract of Balanites aegyptiaca L. Delile Fruit Pulp on Monosodium Glutamate-Induced Excitotoxicity in Swiss Mice , 2022, Evidence-based complementary and alternative medicine : eCAM.

[16]  Lujun Yang,et al.  Effects of caloric overload before caloric restriction in the murine heart , 2022, Aging.

[17]  E. Heist,et al.  The Obesity Paradox in Real-World Nation-Wide Cohort of Patients Admitted for a Stroke in the U.S. , 2022, Journal of clinical medicine.

[18]  T. Cole,et al.  Exploring an algorithm to harmonize International Obesity Task Force and World Health Organization child overweight and obesity prevalence rates , 2022, Pediatric obesity.

[19]  L. Ostrowska,et al.  Secretome of Adipose Tissue as the Key to Understanding the Endocrine Function of Adipose Tissue , 2022, International journal of molecular sciences.

[20]  Y. Fujimura,et al.  The combined effect of green tea and α-glucosyl hesperidin in preventing obesity: a randomized placebo-controlled clinical trial , 2021, Scientific Reports.

[21]  K. Suchacki,et al.  Nutritional Regulation of Human Brown Adipose Tissue , 2021, Nutrients.

[22]  Zhibin Liu,et al.  Aroma and catechin profile and in vitro antioxidant activity of green tea infusion as affected by submerged fermentation with Wolfiporia cocos (Fu Ling). , 2021, Food chemistry.

[23]  J. Oka,et al.  Cognitive and hippocampal synaptic profiles in monosodium glutamate-induced obese mice , 2020, Neuroscience Research.

[24]  T. Behl,et al.  Implicating the effect of ketogenic diet as a preventive measure to obesity and diabetes mellitus. , 2020, Life sciences.

[25]  Rajwinder Kaur,et al.  Pleotropic Effects of Polyphenols in Cardiovascular System. , 2020, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[26]  M. Bilal,et al.  Stimuli-Responsive Polymeric Nanocarriers for Drug Delivery, Imaging, and Theragnosis , 2020, Polymers.

[27]  L. Sciacca,et al.  Adipose Tissue, Obesity and Adiponectin: Role in Endocrine Cancer Risk , 2019, International journal of molecular sciences.

[28]  K. Nakadate,et al.  Small intestine barrier function failure induces systemic inflammation in monosodium glutamate-induced chronically obese mice. , 2019, Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme.

[29]  J. Dvořáčková,et al.  Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2018 Jun; 162(2) , 2018 .

[30]  O. Froy,et al.  The Circadian Clock in White and Brown Adipose Tissue: Mechanistic, Endocrine, and Clinical Aspects. , 2018, Endocrine reviews.

[31]  H. Onda,et al.  Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 Expression , 2017, The Journal of Immunology.

[32]  F. Santini,et al.  Lipodystrophy and obesity are associated with decreased number of T cells with regulatory function and pro-inflammatory macrophage phenotype , 2017, International Journal of Obesity.

[33]  Y. Katsuragi,et al.  Tea catechin and caffeine activate brown adipose tissue and increase cold-induced thermogenic capacity in humans. , 2017, The American journal of clinical nutrition.

[34]  D. Panagiotakos,et al.  Reduced circulating adiponectin levels are associated with the metabolic syndrome independently of obesity, lipid indices and serum insulin levels: a cross-sectional study , 2016, Lipids in Health and Disease.

[35]  T. Hamaoka,et al.  Daily ingestion of catechin-rich beverage increases brown adipose tissue density and decreases extramyocellular lipids in healthy young women , 2016, SpringerPlus.

[36]  K. Nakadate,et al.  Progressive Depletion of Rough Endoplasmic Reticulum in Epithelial Cells of the Small Intestine in Monosodium Glutamate Mice Model of Obesity , 2016, BioMed research international.

[37]  K. Nakadate,et al.  Protective Efficacy of the Ingestion of Mandarin Orange Containing β-Cryptoxanthin on Lipopolysaccharide-induced Acute Nephritis. , 2016, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

[38]  A. Shimotoyodome,et al.  Green tea catechins enhance norepinephrine-induced lipolysis via a protein kinase A-dependent pathway in adipocytes. , 2015, Biochemical and biophysical research communications.

[39]  S. Kaneko,et al.  Prevention and reversal of lipotoxicity-induced hepatic insulin resistance and steatohepatitis in mice by an antioxidant carotenoid, β-cryptoxanthin. , 2015, Endocrinology.

[40]  M. Sugiura.  [β-Cryptoxanthin and the risk for lifestyle-related disease: findings from recent nutritional epidemiologic studies]. , 2015, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

[41]  M. Portillo,et al.  Pterostilbene, a dimethyl ether derivative of resveratrol, reduces fat accumulation in rats fed an obesogenic diet. , 2014, Journal of agricultural and food chemistry.

[42]  K. Flegal,et al.  Prevalence of childhood and adult obesity in the United States, 2011-2012. , 2014, JAMA.

[43]  Hisanori Wakamatsu,et al.  [Pathological changes in hepatocytes of mice with obesity-induced type 2 diabetes by monosodium glutamate]. , 2014, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

[44]  K. Sugihara,et al.  67-kDa laminin receptor increases cGMP to induce cancer-selective apoptosis. , 2013, The Journal of clinical investigation.

[45]  N. Božina,et al.  Adiponectin level and gene variability are obesity and metabolic syndrome markers in a young population. , 2012, Archives of medical research.

[46]  T. Wynn,et al.  Protective and pathogenic functions of macrophage subsets , 2011, Nature Reviews Immunology.

[47]  A. Sahu.  Intracellular Leptin-Signaling Pathways in Hypothalamic Neurons: The Emerging Role of Phosphatidylinositol-3 Kinase-Phosphodiesterase-3B-cAMP Pathway , 2011, Neuroendocrinology.

[48]  Koji Yamada,et al.  Vitamin A Enhances Antitumor Effect of a Green Tea Polyphenol on Melanoma by Upregulating the Polyphenol Sensing Molecule 67-kDa Laminin Receptor , 2010, PloS one.

[49]  E. Trautwein,et al.  Purified black tea theaflavins and theaflavins/catechin supplements did not affect serum lipids in healthy individuals with mildly to moderately elevated cholesterol concentrations , 2010, European journal of nutrition.

[50]  K. Unno,et al.  Protection of brain and pancreas from high-fat diet: Effects of catechin and caffeine , 2009, Physiology & Behavior.

[51]  T. Hase,et al.  A Catechin‐rich Beverage Improves Obesity and Blood Glucose Control in Patients With Type 2 Diabetes , 2009, Obesity.

[52]  J. Blumberg,et al.  Green tea catechin consumption enhances exercise-induced abdominal fat loss in overweight and obese adults. , 2009, The Journal of nutrition.

[53]  J. Edwards,et al.  Exploring the full spectrum of macrophage activation , 2008, Nature Reviews Immunology.

[54]  J. Mege,et al.  Macrophage Polarization in Bacterial Infections , 2008, The Journal of Immunology.

[55]  Yuriko Tanaka,et al.  Catechin Safely Improved Higher Levels of Fatness, Blood Pressure, and Cholesterol in Children , 2008, Obesity.

[56]  U. Heinemann,et al.  Consensus meeting: monosodium glutamate – an update , 2007, European Journal of Clinical Nutrition.

[57]  M. Thun,et al.  American Cancer Society Guidelines on Nutrition and Physical Activity for Cancer Prevention: Reducing the Risk of Cancer With Healthy Food Choices and Physical Activity , 2002, CA: a cancer journal for clinicians.

[58]  J. Viña,et al.  Structure of the blood-brain barrier and its role in the transport of amino acids. , 2006, The Journal of nutrition.

[59]  K. Kotani,et al.  Tea Catechins with a Galloyl Moiety Reduce Body Weight and Fat , 2005 .

[60]  T. Kakuda,et al.  Tea catechins with a galloyl moiety suppress postprandial hypertriacylglycerolemia by delaying lymphatic transport of dietary fat in rats. , 2005, The Journal of nutrition.

[61]  Y. Ikoma,et al.  Recent Progress in -Cryptoxanthin Research , 2005 .

[62]  Y. Ikoma,et al.  Multiple Linear Regression Analysis of the Seasonal Changes in the Serum Concentration of β-Cryptoxanthin , 2004 .

[63]  Y. Fujimura,et al.  A receptor for green tea polyphenol EGCG , 2004, Nature Structural &Molecular Biology.

[64]  D. Reid,et al.  Nutritional associations with bone loss during the menopausal transition: evidence of a beneficial effect of calcium, alcohol, and fruit and vegetable nutrients and of a detrimental effect of fatty acids. , 2004, The American journal of clinical nutrition.

[65]  A. Sahu.  Leptin signaling in the hypothalamus: emphasis on energy homeostasis and leptin resistance , 2003, Frontiers in Neuroendocrinology.

[66]  S. New.  Intake of fruit and vegetables: implications for bone health , 2003, Proceedings of the Nutrition Society.

[67]  A. Sugimoto,et al.  Heat-epimerized tea catechins rich in gallocatechin gallate and catechin gallate are more effective to inhibit cholesterol absorption than tea catechins rich in epigallocatechin gallate and epicatechin gallate. , 2003, Journal of agricultural and food chemistry.

[68]  T. Murase,et al.  Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver , 2002, International Journal of Obesity.

[69]  A. Nagao,et al.  Serum Concentration of β-Cryptoxanthin in Japan Reflects the Frequency of Satsuma Mandarin (Citrus unshiu Marc.) Consumption , 2002 .

[70]  A. Bamedi,et al.  Carotenoid esters in vegetables and fruits: a screening with emphasis on beta-cryptoxanthin esters. , 2001, Journal of agricultural and food chemistry.

[71]  T. Maoka,et al.  Modifying effects of carotenoids on superoxide and nitric oxide generation from stimulated leukocytes. , 2000, Cancer letters.

[72]  W. Stahl,et al.  β-Cryptoxanthin Selectively Increases in Human Chylomicrons upon Ingestion of Tangerine Concentrate Rich in β-Cryptoxanthin Esters , 1995 .

[73]  M. Sugano,et al.  Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. , 1992, Biochimica et biophysica acta.

[74]  W. Stahl,et al.  cis-trans isomers of lycopene and β-carotene in human serum and tissues , 1992 .

[75]  J. Erdman,et al.  Concentrations of selected carotenoids and vitamin A in human liver, kidney and lung tissue. , 1991, The Journal of nutrition.

[76]  S. Tanumihardjo,et al.  Retinyl ester (vitamin A ester) and carotenoid composition in human liver. , 1990, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[77]  L. Kaplan,et al.  Carotenoid composition, concentrations, and relationships in various human organs. , 1990, Clinical physiology and biochemistry.