Optical waveguide biosensors constructed with subwavelength gratings.

The reflection resonance spectrum of a subwavelength diffraction-grating-coupled waveguide is used to analyze biomolecular interactions in real time. By detecting this resonance wavelength shift, the optical waveguide biosensor provides the ability to identify the kinetics of the biomolecular interaction on an on-line basis without the need for extrinsic labeling of the biomolecules. A theoretical analysis of the subwavelength optical waveguide biosensor is performed. A biosensor with a narrow reflection resonance spectrum, and hence an enhanced detection resolution, is then designed and fabricated. Currently, the detection limit of the optical waveguide sensor is approximately 10(-5) refractive-index units. The biosensor is successfully applied to study of the dynamic response of an antibody interaction with protein G adsorbed on the sensing surface.

[1]  W. Lukosz,et al.  Integrated optical switches and gas sensors. , 1984, Optics letters.

[2]  B. Cunningham,et al.  Colorimetric resonant reflection as a direct biochemical assay technique , 2002, Technical Digest. MEMS 2002 IEEE International Conference. Fifteenth IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.02CH37266).

[3]  R. Kunz,et al.  Label-free highly sensitive detection of (small) molecules by wavelength interrogation of integrated optical chips , 2003 .

[4]  Raymond K. Kostuk,et al.  Effective-medium theory of zeroth-order lamellar gratings in conical mountings , 1993 .

[5]  Ping Sheng,et al.  Exact eigenfunctions for square-wave gratings: Application to diffraction and surface-plasmon calculations , 1982 .

[6]  R. Wood.  XLII. On a remarkable case of uneven distribution of light in a diffraction grating spectrum , 1902 .

[7]  R. Magnusson,et al.  New principle for optical filters , 1992 .

[8]  J. Huignard,et al.  Self-induced coherent oscillations with photorefractive Bi(12)SiO(20) amplifier. , 1985, Optics letters.

[9]  R E Kunz,et al.  Wavelength-interrogated optical sensor for biochemical applications. , 2000, Optics letters.

[10]  S. T. Peng,et al.  Rigorous formulation of scattering and guidance by dielectric grating waveguides: general case of oblique incidence , 1989 .

[11]  Koichi Iwata,et al.  Effective medium theory of two-dimensional subwavelength gratings in the non-quasi-static limit , 1998 .

[12]  W. Lukosz,et al.  Sensitivity of grating couplers as integrated-optical chemical sensors , 1989 .

[13]  R. Wood,et al.  On a Remarkable Case of Uneven Distribution of Light in a Diffraction Grating Spectrum , 1902 .

[14]  Charles V. Shank,et al.  Grating filters for thin‐film optical waveguides , 1974 .

[15]  S. Peng,et al.  Experimental demonstration of resonant anomalies in diffraction from two-dimensional gratings. , 1996, Optics letters.

[16]  A. Friesem,et al.  Resonant grating waveguide structures , 1997 .

[17]  T. Gaylord,et al.  Rigorous coupled-wave analysis of planar-grating diffraction , 1981 .

[18]  Amnon Yariv,et al.  Optical Waves in Crystals: Propagation and Control of Laser Radiation , 1983 .

[19]  A. A. Oliner,et al.  A New Theory of Wood’s Anomalies on Optical Gratings , 1965 .

[20]  Theodor Tamir,et al.  Theory of Periodic Dielect Waveguides , 1975 .

[21]  G. Michael Morris,et al.  Resonant scattering from two-dimensional gratings , 1996 .

[22]  Thomas K. Gaylord,et al.  Rigorous coupled-wave analysis of grating diffraction— E-mode polarization and losses , 1983 .

[23]  Amnon Yariv,et al.  Periodic structures for integrated optics , 1977 .