Minimal influence of water and nutrient content on the bacterial community composition of a maritime Antarctic soil.

Bacterial community composition was determined by culture-independent PCR-based methods in two soils differing markedly in their water, C, N and P contents sampled from Mars Oasis on Alexander Island, western Antarctic Peninsula. 16S rRNA sequences of the phyla Actinobacteria, Cyanobacteria, α-Proteobacteria and Acidobacteria were commonly (> 8% frequency) obtained from soil. Those of β-, γ- and δ-Proteobacteria, Chloroflexi, Bacteroidetes, Verrucomicrobia, Planctomycetes, Gemmatimonadetes and Firmicutes were less frequent. Comparisons of slopes of collector's curves and the Shannon-Weiner diversity index indicated no difference in overall bacterial diversity between the two soils, although sequences of δ-Proteobacteria and the cyanobacterial genus Leptolyngbya were more commonly derived from the soil with the higher water and nutrient content. The data suggest that different levels of soil water, C, N and P have only a minor effect on the bacterial community composition of maritime Antarctic soils.

[1]  J. Tiedje,et al.  Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA. , 1997, Microbiology.

[2]  J. Harris,et al.  Factors Affecting Bacterial Productivity in Soils on Isolated Inland Nunataks in Continental Antarctica , 1997, Microbial Ecology.

[3]  M. Häggblom,et al.  Bacterial communities in Arctic fjelds of Finnish Lapland are stable but highly pH-dependent. , 2007, FEMS microbiology ecology.

[4]  D. Wynn-Williams.  Response of pioneer soil microalgal colonists to environmental change in Antarctica , 1996, Microbial Ecology.

[5]  W. Stafford,et al.  Bacterial Diversity in Three Different Antarctic Cold Desert Mineral Soils , 2006, Microbial Ecology.

[6]  M. C. Davey.  Effects of continuous and repeated dehydration on carbon fixation by bryophytes from the maritime Antarctic , 1997, Oecologia.

[7]  R. Aerts,et al.  Size and structure of bacterial, fungal and nematode communities along an Antarctic environmental gradient. , 2006, FEMS microbiology ecology.

[8]  A. D. Kennedy.  Water as a Limiting Factor in the Antarctic Terrestrial Environment: A Biogeographical Synthesis , 1993 .

[9]  M. Worland,et al.  Survival and water loss in some Antarctic arthropods , 1986 .

[10]  D. Walton,et al.  A statistical analysis of the relationships among viable microbial populations, vegetation, and environment in a subantarctic tundra , 1985, Microbial Ecology.

[11]  K. Schleifer,et al.  Detection and in situ identification of representatives of a widely distributed new bacterial phylum. , 1997, FEMS microbiology letters.

[12]  A. Camacho,et al.  Community structure and physiological characterization of microbial mats in Byers Peninsula, Livingston Island (South Shetland Islands, Antarctica). , 2007, FEMS microbiology ecology.

[13]  J. Aislabie,et al.  Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica. , 2005, FEMS microbiology ecology.

[14]  R. B. Jackson,et al.  The diversity and biogeography of soil bacterial communities. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Aislabie,et al.  Dominant bacteria in soils of Marble Point and Wright Valley, Victoria Land, Antarctica , 2006 .

[16]  R. Margalef,et al.  Information theory in ecology , 1958 .

[17]  W. Pearson.  Rapid and sensitive sequence comparison with FASTP and FASTA. , 1990, Methods in enzymology.

[18]  P. Convey,et al.  The cyanobacterial community of polygon soils at an inland Antarctic nunatak , 2007, Polar Biology.

[19]  H. Blöcker,et al.  Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. , 1989, Nucleic acids research.

[20]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[21]  K. Hughes,et al.  A novel Antarctic microbial endolithic community within gypsum crusts. , 2003, Environmental microbiology.

[22]  K. Newsham,et al.  Soil fungal community composition at Mars Oasis, a southern maritime Antarctic site, assessed by PCR amplification and cloning , 2009 .

[23]  C. Kuske,et al.  Assessment of Microbial Diversity in Four Southwestern United States Soils by 16S rRNA Gene Terminal Restriction Fragment Analysis , 2000, Applied and Environmental Microbiology.

[24]  H. Heuer,et al.  Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients , 1997, Applied and environmental microbiology.

[25]  B. Elberling,et al.  Distribution and dynamics of soil organic matter in an Antarctic dry valley , 2006 .