Expression of engrailed during segmentation in grasshopper and crayfish.

We have used a monoclonal antibody that recognizes engrailed proteins to compare the process of segmentation in grasshopper, crayfish, and Drosophila. Drosophila embryos rapidly generate metameres during an embryonic stage characterized by the absence of cell division. In contrast, many other arthropod embryos, such as those of more primitive insects and crustaceans, generate metameres gradually and sequentially, as cell proliferation causes caudal elongation. In all three organisms, the pattern of engrailed expression at the segmented germ band stage is similar, and the parasegments are the first metameres to form. Nevertheless, the way in which the engrailed pattern is generated differs and reflects the differences in how these organisms generate their metameres. These differences call into question what role homologues of the Drosophila pair-rule segmentation genes might play in other arthropods that generate metameres sequentially.

[1]  Michael Akam,et al.  Homeotic genes and the control of segment diversity , 1988 .

[2]  P. O’Farrell,et al.  The engrailed locus of drosophila: In situ localization of transcripts reveals compartment-specific expression , 1985, Cell.

[3]  Judith A. Kassis,et al.  Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis , 1988, Nature.

[4]  M P Weir,et al.  Patterns of engrailed protein in early Drosophila embryos. , 1989, Development.

[5]  E. Hafen,et al.  Spatial distribution of transcripts from the segmentation gene fushi tarazu during Drosophila embryonic development , 1984, Cell.

[6]  W. McGinnis,et al.  Isolation of a homoeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts , 1985, Nature.

[7]  C Q Doe,et al.  Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis. , 1988, Science.

[8]  T. Kornberg,et al.  Patterns of engrailed and fushi tarazu transcripts reveal novel intermediate stages in Drosophila segmentation , 1985, Nature.

[9]  V. French,et al.  Disruption of segmentation in a short germ insect embryo. II. The structure of segmental abnormalities induced by heat shock. , 1986, Journal of embryology and experimental morphology.

[10]  P. O’Farrell,et al.  Establishment and refinement of segmental pattern in the Drosophila embryo: spatial control of engrailed expression by pair-rule genes. , 1987, Genes & development.

[11]  P. Lawrence,et al.  Parasegments and compartments in the Drosophila embryo , 1985, Nature.

[12]  C. H. Waddington,et al.  Developmental systems : insects , 1972 .

[13]  K. Sander.  Specification of the Basic Body Pattern in Insect Embryogenesis1 , 1976 .

[14]  M. Bastiani,et al.  Pathfinding by neuronal growth cones in grasshopper embryos. II. Selective fasciculation onto specific axonal pathways , 1983, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[15]  A. Simcox,et al.  When does determination occur in Drosophila embryos? , 1983, Developmental biology.

[16]  S. Carroll,et al.  Localization of the fushi tarazu protein during Drosophila embryogenesis , 1985, Cell.

[17]  H. Keshishian,et al.  Quantitative staging of embryonic development of the grasshopper, Schistocerca nitens. , 1979, Journal of embryology and experimental morphology.

[18]  K. G. Coleman,et al.  Expression of engrailed proteins in arthropods, annelids, and chordates. , 1989, Cell.

[19]  P. Ingham.  The molecular genetics of embryonic pattern formation in Drosophila , 1988, Nature.

[20]  K. Sander.  Studies in insect segmentation: from teratology to phenogenetics , 1988 .

[21]  M. Bastiani,et al.  From grasshopper to Drosophila: a common plan for neuronal development , 1984, Nature.

[22]  C. Goodman,et al.  Control of neuronal fate by the Drosophila segmentation gene even-skipped , 1988, Nature.

[23]  P. O’Farrell,et al.  Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein , 1985, Cell.