It seems improbable that leucocyte oxidase is a fatty acid peroxide, as suggested by Sehrt, on these grounds: 1. Positive reactions for aldehyde and for ethylene groups are not obtained. Hence the presence of fatty acid peroxides in leucocytes seems unlikely. 2. Peroxidase has been isolated from leucocytes by Agner. Histochemical behavior on treatment with adverse reagents and with heat indicates similarity of benzidine peroxidase and of indophenol oxidase, but dissimilarity of these from the sudanophil substance in leucocytes. 3. Sudan dyes demonstrate or produce in neutrophil leucocytes deeply colored granules of quite variable size, and the size of the granules apparently increases with the duration of staining. 4. The demonstration of sudanophil granules in leucocytes is accomplished with difficulty. The colored granules are highly stable toward many Sudan dye solvents. Decolorization is difficult and recoloration is uncertain. 5. True fats and lipoids, after staining with Sudan dyes, are readily decolorized by appropriate dye solvents and are restainable in apparently undiminished amounts. 6. Some oxidants tend to impair sudanophilia of leucocytes. Potassium bichromate improves the preservation of sudanophil lipoids, as in the Ciaccio method, but destroys the sudanophilia of polymorphonuclear leucocytes. 7. Hydrogen peroxide apparently possesses an inconstant and irregular, though sometimes quite pronounced, accelerating effect on the production of Sudan stained granules in leucocytes. 8. The sudanophilia of leucocytes depends on an as yet unexplained chemical combination of the dyes with cytoplasmic constituents which are probably not in the form of preexisting granules, rather than on lipoid staining.
[1]
R. Lillie.
Ethylenic reaction of ceroid with performic acid and Schiff reagent.
,
1952,
Stain technology.
[2]
Lillie Rd.
Histochemical comparison of the Casella, Bauer, and periodic acid oxidation-Schiff leucofuchsin technics.
,
1951
.
[3]
R. Lillie,et al.
Histochemical comparison of the Casella, Bauer, and periodic acid oxidation-Schiff leucofuchsin technics.
,
1951,
Stain technology.
[4]
R. Harrison,et al.
Cytological and histochemical variations in the adrenal cortex of the albino rat.
,
1950,
Journal of anatomy.
[5]
R. N. Baillif,et al.
Studies on leucocyte granules after staining with sudan black B and May-Grünwald Giemsa.
,
1947,
The Journal of laboratory and clinical medicine.
[6]
H. Sheehan,et al.
An improved method of staining leucocyte granules with Sudan black B.
,
1947,
The Journal of pathology and bacteriology.
[7]
E. Dempsey,et al.
Observations on the chemical cytology of normal blood and hemopoietic tissues
,
1946,
The Anatomical record.
[8]
G. Discombe.
The nature of neutrophilic granulation.
,
1946,
The Journal of pathology and bacteriology.
[9]
H. Sheehan.
The staining of leucocyte granules by sudan black B
,
1939
.
[10]
P. Bacsich.
On the Staining of Lipoid Granules in Leucocytes.
,
1936,
Journal of anatomy.
[11]
E. M. Boyd.
The lipid content of the white blood cells in normal young women.
,
1933
.
[12]
G. Levi.
Trattato di istologia
,
1927
.