Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets

Dentro de la rehabilitacion oral, es importante preservar el reborde alveolar post exodoncia. Se expone un analisis histologico, histoquimico e histomorfometrico de dos casos clinicos de terapias de regeneracion osea de alveolos post extraccion mediante FDBA. En dos pacientes se extrajeron piezas dentarias destruidas y se indujo regeneracion osea mediante FDBA. Seis meses despues, se obtuvo una biopsia del injerto y mediante un implante de titanio fue rehabilitado. Para el analisis Histologico e Histomorfometrico, las muestras fueron tratadas con las tecnicas Hematoxilina-Eosina, Azul de Alcian, Masson, Von Kossa y colorante Picrosirius de Junqueira.Se midio el area total de tejido, asi como el area de tejido oseo. Las superficies de hueso de las muestras se observaron inactivas, no fueron encontrados osteoblastos ni osteoclastos, solo osteocitos y celulas osteogenas, lo que no significa que el hueso este en estado quiescente, sino mas bien a que su genesis ocurre a partir de la matriz de tejido donde se encuentran celulas con potencialidad de formar osteocitos indiferenciados y un microambiente con proteinas de la familia de factor de crecimiento transformante beta. No fueron encontradas particulas de biomaterial inactivo. En las muestras se cuantifico 0% y 30% de hueso mineralizado. Dadas las diferencias histologicas encontradas con otros estudios, es necesario profundizar el conocimiento en los procesos involucrados en la regeneracion osea dependiendo del biomaterial utilizado, y la calidad osea resultante en cada procedimiento en particular sin perder de vista la variabilidad que puede presentarse dependiendo de cada caso clinico.

[1]  D. Kim,et al.  Minimally invasive alveolar ridge augmentation procedure (tunneling technique) using rhPDGF-BB in combination with three matrices: a case series. , 2009, The International journal of periodontics & restorative dentistry.

[2]  M. Dard,et al.  Ridge augmentation and maxillary sinus grafting with a biphasic calcium phosphate: histologic and histomorphometric observations. , 2009, Clinical oral implants research.

[3]  R. F. Fernández,et al.  Recuperación de la Morfología y Fisiología Maxilo Mandibular: Biomateriales en Regeneración Ósea , 2008 .

[4]  H. Tal,et al.  Histomorphometric analysis of newly formed bone after maxillary sinus floor augmentation using ground cortical bone allograft and internal collagen membrane. , 2008, Journal of periodontology.

[5]  M. Chiapasco,et al.  Long-term results of mandibular reconstruction with autogenous bone grafts and oral implants after tumor resection. , 2008, Clinical oral implants research.

[6]  T. Gerber,et al.  Immunohistochemical characterization of nanocrystalline hydroxyapatite silica gel (NanoBone(r)) osteogenesis: a study on biopsies from human jaws. , 2008, Clinical oral implants research.

[7]  F. Fontana,et al.  Clinical outcomes of vertical bone augmentation to enable dental implant placement: a systematic review. , 2008, Journal of clinical periodontology.

[8]  R. Kao,et al.  Simultaneous hard and soft tissue augmentation for implants in the esthetic zone: report of 37 consecutive cases. , 2008, Journal of periodontology.

[9]  C. Hämmerle,et al.  Advances in bone augmentation to enable dental implant placement: Consensus Report of the Sixth European Workshop on Periodontology. , 2008, Journal of clinical periodontology.

[10]  V. Chadha,et al.  Clinical outcomes of implants following lateral bone augmentation: systematic assessment of available options (barrier membranes, bone grafts, split osteotomy). , 2008, Journal of clinical periodontology.

[11]  P. Galindo-Moreno,et al.  Clinical and histologic comparison of two different composite grafts for sinus augmentation: a pilot clinical trial. , 2008, Clinical oral implants research.

[12]  Seong-Ho Choi,et al.  Histologic and clinical evaluation for maxillary sinus augmentation using macroporous biphasic calcium phosphate in human. , 2008, Clinical oral implants research.

[13]  M. Dard,et al.  Histomorphometric assessment of bone formation in sinus augmentation utilizing a combination of autogenous and hydroxyapatite/biphasic tricalcium phosphate graft materials: at 6 and 9 months in humans. , 2008, Clinical oral implants research.

[14]  S. Lynch,et al.  Clinical results using recombinant human platelet-derived growth factor and mineralized freeze-dried bone allograft in periodontal defects. , 2007, The International journal of periodontics & restorative dentistry.

[15]  Barbara D Boyan,et al.  Clinical evaluation of demineralized bone allograft in a hyaluronic acid carrier for sinus lift augmentation in humans: a computed tomography and histomorphometric study. , 2007, Clinical oral implants research.

[16]  M. Chiapasco,et al.  Augmentation procedures for the rehabilitation of deficient edentulous ridges with oral implants. , 2006, Clinical oral implants research.

[17]  C. Ozdoba,et al.  Changes in the volume and density of calvarial split bone grafts after alveolar ridge augmentation. , 2006, Clinical oral implants research.

[18]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[19]  Z. Suba,et al.  Maxillary sinus floor grafting with beta-tricalcium phosphate in humans: density and microarchitecture of the newly formed bone. , 2006, Clinical oral implants research.

[20]  N. Baldini,et al.  In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination , 2005, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[21]  J. Hatch,et al.  Histologic evaluation of mineralized and demineralized freeze-dried bone allograft for ridge and sinus augmentations. , 2005, The International journal of periodontics & restorative dentistry.

[22]  M. Reynolds,et al.  Evaluation of the Adjunctive Benefits of Platelet-Rich Plasma in Subantral Sinus Augmentation , 2005, The Journal of craniofacial surgery.

[23]  R. Mericske-Stern,et al.  Extensive augmentation of the alveolar ridge using autogenous calvarial split bone grafts for dental rehabilitation. , 2004, Clinical oral implants research.

[24]  E. Odell,et al.  Efficacy of bovine bone mineral for alveolar augmentation: a human histologic study. , 2003, Clinical oral implants research.

[25]  X. Dereka,et al.  Human periodontal ligament cell responses to recombinant human bone morphogenetic protein-2 with and without bone allografts. , 2003, Journal of periodontology.

[26]  H. Greenwell,et al.  Ridge preservation with freeze-dried bone allograft and a collagen membrane compared to extraction alone for implant site development: a clinical and histologic study in humans. , 2003, Journal of periodontology.

[27]  J. Mellonig,et al.  Clinical and histologic evaluation of bone-replacement grafts in the treatment of localized alveolar ridge defects. Part 1: Mineralized freeze-dried bone allograft. , 2003, The International journal of periodontics & restorative dentistry.

[28]  Luca Cordaro,et al.  Clinical results of alveolar ridge augmentation with mandibular block bone grafts in partially edentulous patients prior to implant placement. , 2002, Clinical oral implants research.

[29]  D. Pashley,et al.  The effects of varying degrees of allograft decalcification on cultured porcine osteoclast cells. , 2002, Journal of periodontology.

[30]  P. Missika,et al.  A prospective randomized study comparing two techniques of bone augmentation: onlay graft alone or associated with a membrane. , 2001, Clinical oral implants research.

[31]  E. Burger,et al.  Histology of human alveolar bone regeneration with a porous tricalcium phosphate. A report of two cases. , 2001, Clinical oral implants research.

[32]  M. Reynolds,et al.  Guided bone regeneration for dehiscence and fenestration defects on implants using an absorbable polymer barrier. , 2001, Journal of periodontology.

[33]  L. Laurell,et al.  Treatment of intrabony defects by different surgical procedures. A literature review. , 1998, Journal of periodontology.

[34]  N. Baldini,et al.  In vitro evaluation of freeze-dried bone allografts combined with platelet rich plasma and human bone marrow stromal cells for tissue engineering , 2009, Journal of materials science. Materials in medicine.

[35]  M. Chiapasco,et al.  Bone Ceramic: histomorphometric results from a randomized controlled multicenter clinical trial , 2008 .

[36]  M. Sharawy,et al.  The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium. , 2006, The Journal of oral implantology.

[37]  S. Vastardis,et al.  Comparative evaluation of decalcified and non-decalcified freeze-dried bone allografts in rhesus monkeys. I. Histologic findings. , 2005, Journal of periodontology.

[38]  M. Filstein,et al.  Histological evaluation of sinus augmentation using platelet rich plasma (PRP): a case series. , 2001, Journal of the International Academy of Periodontology.