A study on the improvement iron nutrition of peanut intercropping with maize on nitrogen fixation at early stages of growth of peanut on a calcareous soil

Abstract A glasshouse study employing a split-root technique was conducted to investigate the influence of intercropping with maize (Zea mays L.) in a calcareous soil on N2 fixation by peanut (Arachis hypogaea L.) at early stages of growth. In this intercropping system, competitive interactions between maize and peanut for N and improvement of Fe uptake were likely to be important factors affecting N2 fixation of peanut. The experiment was comprised of three treatments which included treatment I: peanut monocropping; treatment II: maize/peanut intercropping (the major and the minor compartments with low N, 50 mg kg−1); treatment III: maize/peanut intercropping (the major compartment with low N, 50 mg kg−1 and the minor compartment with high, N 200 mg kg−1). The minor compartment of treatment III was fertilized with 200 mg kg−1 N for reducing or eliminating the competition of N coming from intercropping maize. Intercropping with maize corrected Fe chlorosis of peanut by significantly increasing plant Fe concentration and uptake. Compared with the monocropping treatment, iron uptake increased from intercropping treatment II and III by 22 and 24% per plant, 30 and 29% shoots, 38 and 60% nodules. Iron uptake by the root nodules was especially enhanced in the intercropping system. In contrast, intercropping with maize had little effect on NO3 −1-N concentrations in the soil rhizosphere of peanut or on N concentrations and uptake by peanut compared with plants in monoculture. The results indicate that the improvement in Fe nutrition was an important factor promoting N2 fixation by peanut in the intercropping system at the flowering stage of peanut growth, and that competition for N by intercropped maize had little effect on N2 fixation by peanut under the experimental conditions.

[1]  D. Herridge,et al.  Measurement of N2 fixation in maize (Zea mays L.)—ricebean (Vigna umbellata [Thunb.] Ohwi and Ohashi) intercrops , 1988, Plant and Soil.

[2]  M. Guerinot,et al.  A Dominant-Negative fur Mutation in Bradyrhizobium japonicum , 2004, Journal of bacteriology.

[3]  Fusuo Zhang,et al.  Studies on the improvement in iron nutrition of peanut by intercropping with maize on a calcareous soil , 2000, Plant and Soil.

[4]  E. S. Jensen Grain yield, symbiotic N2 fixation and interspecific competition for inorganic N in pea-barley intercrops , 1996, Plant and Soil.

[5]  Marianne Karpenstein-Machan,et al.  Biomass yield and nitrogen fixation of legumes monocropped and intercropped with rye and rotation effects on a subsequent maize crop , 2004, Plant and Soil.

[6]  J. Ladha,et al.  Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production? , 1995, Plant and Soil.

[7]  R. Terry,et al.  The role of active Bradyrhizobium japonicum in iron stress response of soybeans , 2004, Plant and Soil.

[8]  Fusuo Zhang,et al.  Iron Nutrition of Peanut Enhanced by Mixed Cropping with Maize: Possible Role of Root Morphology and Rhizosphere Microflora , 2003 .

[9]  Rowena Thomson,et al.  The soybean NRAMP homologue, GmDMT1, is a symbiotic divalent metal transporter capable of ferrous iron transport. , 2003, The Plant journal : for cell and molecular biology.

[10]  G Sawers,et al.  Fur is not the global regulator of iron uptake genes in Rhizobium leguminosarum. , 2003, Microbiology.

[11]  M. Wexler,et al.  Metals and the rhizobial-legume symbiosis--uptake, utilization and signalling. , 2001, Advances in microbial physiology.

[12]  F. Dakora Nodule Function in Symbiotic Bambara Groundnut (Vigna subterraneaL.) and Kersting's Bean (Macrotyloma geocarpumL.) is Tolerant of Nitrate in the Root Medium , 1998 .

[13]  S. Tobita,et al.  Nitrogen fertilizer management in pigeonpea/sorghum intercropping on an Alfisol in the semi-arid tropics , 1997 .

[14]  S. Mori,et al.  Light-Dependent Iron Transport into Isolated Barley Chloroplasts , 1997 .

[15]  M. Guerinot,et al.  Iron Uptake by Symbiosomes from Soybean Root Nodules , 1996, Plant physiology.

[16]  A. Millar,et al.  Specificity of the Organic Acid Activation of Alternative Oxidase in Plant Mitochondria , 1996, Plant physiology.

[17]  L. Materon,et al.  Competition between Medicago truncatula and wheat for 15N labeled soil nitrogen and influence of phosphorus , 1996 .

[18]  F. Dakora A functional relationship between leghaemoglobin and nitrogenase based on novel measurements of the two proteins in legume root nodules. , 1995, Annals of botany.

[19]  Caixian Tang,et al.  The role of iron in the (Brady) rhizobium-legume symbiosis , 1992 .

[20]  Caixian Tang,et al.  A split‐root experiment shows that iron is required for nodule initiation in Lupinus angustifolius L. , 1990 .

[21]  S. Saxena,et al.  Symbiotic nitrogen fixation and nitrogen benefits by nodulated soybean (Glycine max (L.) Merrill) to interplanted crops in northern India , 1989 .

[22]  R. Bell,et al.  Response to bradyrhizobium strain of peanut cultivars grown under iron stress , 1988 .

[23]  A. Hemantaranjan Iron fertilization In relation to nodulation and nitrogen fixation in French bean (Phaseolus vulgaris L.) , 1988 .

[24]  M. Dilworth,et al.  Iron-deficiency specifically limits nodule development in peanut inoculated with Bradyrhizobium sp. , 1988, The New phytologist.

[25]  P. Wong,et al.  Inhibition of legume nodule formation and N2 fixation by nitrate , 1988 .

[26]  H. Bienfait,et al.  Free space iron pools in roots: generation and mobilization. , 1985, Plant physiology.

[27]  S. Choudhury,et al.  Iron nutrition and symbiotic N2‐fixation of lentil (lens culinaris) genotypes in calcareous soil , 1984 .

[28]  N. Terry,et al.  Limiting Factors in Photosynthesis: IV. Iron Stress-Mediated Changes in Light-Harvesting and Electron Transport Capacity and its Effects on Photosynthesis in Vivo. , 1983, Plant physiology.

[29]  S. Singh,et al.  Effect of pressmud amended pyrite on symbiotic N2‐fixation, active iron contents of nodules, grain yield and quality of chick pea (cicer arietinum Linn.) Genotypes in calcareous soil , 1982 .

[30]  J. Pushnik,et al.  The effects of iron and light treatments on chloroplast composition and ultrastructure in iron‐deficient barley leaves , 1982 .

[31]  G. Schuman,et al.  Automated Total Nitrogen Analysis of Soil and Plant Samples1 , 1973 .