Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein.

A complementary DNA (cDNA) of 2559 bp which encode all 674 amino acids of mouse protein kinase C-delta (PKC-delta) has been isolated from a cDNA library prepared from ABPL-2, a mouse myeloid tumor. The library was screened with a partial PKC-delta cDNA clone that had been created by polymerase chain reaction (PCR) amplification of ABPL-2 RNA using primers that are conserved among all rat PKC isozymes. This approach proved to be a distinct improvement over screening with synthetic oligonucleotides. Similar sets of cDNAs prepared from other hemopoietic cell lines were screened with this PKC-delta cDNA and with probes for the other PKC isoforms. These experiments revealed that the major isoform of PKC expressed in hemopoietic cells is PKC-delta. PKC-delta protein was purified from ABPL-3, a mouse myeloid tumor which expressed principally the delta isoform of PKC. The protein eluted from a hydroxylapatite column in the same position as PKC-beta and -epsilon would elute, if present. The kinase activity of purified PKC-delta showed strict dependence on the presence of phospholipids, but showed no activation by Ca2+.

[1]  Y. Nishizuka,et al.  The molecular heterogeneity of protein kinase C and its implications for cellular regulation , 1988, Nature.

[2]  R. Kriz,et al.  Cloning and expression of multiple protein kinase C cDNAs , 1986, Cell.

[3]  F. Marks,et al.  Purification and characterization of a calcium-unresponsive, phorbol ester/phospholipid-activated protein kinase from porcine spleen. , 1990, The Journal of biological chemistry.

[4]  J. Mushinski,et al.  Activation of cellular oncogenes in human and mouse leukemia-lymphomas: spontaneous and induced oncogene expression in murine B lymphocytic neoplasms. , 1987, Cancer investigation.

[5]  B. Strulovici,et al.  Activation of distinct protein kinase C isozymes by phorbol esters: correlation with induction of interleukin 1 beta gene expression. , 1989, Biochemistry.

[6]  C. Dani,et al.  Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. , 1985, Nucleic acids research.

[7]  Y. Nishizuka,et al.  Protein kinase C zeta subspecies from rat brain: its structure, expression, and properties. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[8]  F. Huang,et al.  Isozymic forms of rat brain Ca2+-activated and phospholipid-dependent protein kinase. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[9]  K. Suzuki,et al.  A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. , 1990, The Journal of biological chemistry.

[10]  S. Bauer,et al.  DNA rearrangement and altered RNA expression of the c-myb oncogene in mouse plasmacytoid lymphosarcomas. , 1983, Science.

[11]  P. Parker,et al.  Unique substrate specificity and regulatory properties of PKC‐ε: a rationale for diversity , 1989 .

[12]  S. Mizel,et al.  In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C , 1989, Molecular and cellular biology.

[13]  Y. Nishizuka,et al.  The structure, expression, and properties of additional members of the protein kinase C family. , 1988, The Journal of biological chemistry.

[14]  H. Kawasaki,et al.  Enzymatic properties of a novel phorbol ester receptor/protein kinase, nPKC. , 1989, Journal of biochemistry.

[15]  H. Morse,et al.  Phorbol ester-induced growth arrest of murine myelomonocytic leukemic cells with virus-disrupted myb locus is not accompanied by decreased myc and myb expression. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[16]  K. Arai,et al.  Molecular genetic analysis of the regulatory and catalytic domains of protein kinase C. , 1989, The Journal of biological chemistry.

[17]  S. Ohno,et al.  A novel phorbol ester receptor/protein kinase, nPKC, distantly related to the protein kinase C family , 1988, Cell.

[18]  Y. Nishizuka,et al.  Human T cell activation by phorbol esters and diacylglycerol analogues. , 1989, Journal of immunology.

[19]  E. Appella,et al.  Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor. , 1990, The Journal of biological chemistry.

[20]  Y. Nishizuka.  Studies and perspectives of protein kinase C. , 1986, Science.