An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells

Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d33) of 57.8 pCN−1 was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.

[1]  H. Beige,et al.  Modeling of poling behavior of ferroelectric 1-3 composites , 2001 .

[2]  M L Moss,et al.  The functional matrix hypothesis revisited. 1. The role of mechanotransduction. , 1997, American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics.

[3]  M. Swiontkowski,et al.  Bone-graft substitutes , 1999, The Lancet.

[4]  T. Shimokawa,et al.  In vitro assays for adhesion and migration of osteoblastic cells (Saos-2) on titanium surfaces , 2006, Cell and Tissue Research.

[5]  J. Chaudhuri,et al.  Characterisation of ferroelectric-calcium phosphate composites and ceramics , 2007 .

[6]  Steven G. Wax,et al.  Electroactive polymer actuators and devices , 1999, Smart Structures.

[7]  Andrew A. Marino,et al.  Piezoelectric Effect and Growth Control in Bone , 1970, Nature.

[8]  J. Chaudhuri,et al.  Dielectric and piezoelectric properties of hydroxyapatite-BaTiO3 composites , 2006 .

[9]  R. Legeros,et al.  Properties of osteoconductive biomaterials: calcium phosphates. , 2002, Clinical orthopaedics and related research.

[10]  Serena M. Best,et al.  Bioceramics: Past, present and for the future , 2008 .

[11]  J. Bateman,et al.  Matrix deposition by a calcifying human osteogenic sarcoma cell line (SAOS-2). , 1995, Bone.

[12]  Maxence Bigerelle,et al.  The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour. , 2000, Biomaterials.

[13]  W. J. Merz.  Piezoelectric Ceramics , 1972, Nature.

[14]  A F von Recum,et al.  Piezoelectric ceramic implants: in vivo results. , 1981, Journal of biomedical materials research.

[15]  X Zhang,et al.  Promotion of osteogenesis by a piezoelectric biological ceramic. , 1997, Biomaterials.

[16]  Miho Nakamura,et al.  Enhanced bone ingrowth into hydroxyapatite with interconnected pores by Electrical Polarization. , 2006, Biomaterials.

[17]  Pamela Habibovic,et al.  Osteoinductive biomaterials—properties and relevance in bone repair , 2007, Journal of tissue engineering and regenerative medicine.

[18]  Xing‐dong Zhang,et al.  Promotion of osteogenesis by a piezoelectric biological ceramic , 1997 .

[19]  J. Skepper,et al.  Effect of sintered silicate-substituted hydroxyapatite on remodelling processes at the bone-implant interface. , 2004, Biomaterials.

[20]  Ieee Standards Board.  IEEE Standard on Piezoelectricity , 1996 .

[21]  Klemens Rottner,et al.  The lamellipodium: where motility begins. , 2002, Trends in cell biology.

[22]  Eiichi Fukada,et al.  Piezoelectric Effects in Collagen , 1964 .

[23]  Xi-Qiao Feng,et al.  Effects of electric fatigue on the butterfly curves of ferroelectric ceramics , 2007 .

[24]  W. Bonfield,et al.  Bioactivity of ceramic–polymer composites with varied composition and surface topography , 2004, Journal of materials science. Materials in medicine.