Protective role of heme oxygenase‐1 in oxidative stress‐induced neuronal injury

Heme oxygenase‐1 (HO‐1) is a stress protein induced in response to a variety of oxidative challenges. After treatment of the hybrid septal cells SN 56 with β‐amyloid peptide (β‐AP1–40) or hydrogen peroxide (H2O2), we detected high levels of reactive oxygen species, accompanied by a significant elevation in HO‐1 expression. Levels of HO‐1 increased and then decreased following cell loss. Pretreatment of SN 56 cells with HO‐1 antisense oligonucleotides dramatically decreased the immunoreactivity of HO‐1 and significantly enhanced the cytotoxicity of β‐AP1–40 and H2O2. In contrast, pretreatment with hemin, an HO‐1 inducer, increased the expression of HO‐1 and decreased the β‐AP1–40‐ and H2O2‐induced cytotoxicity. These findings support the importance of HO‐1 in protecting neurons against oxidative stress‐induced injury. J. Neurosci. Res. 56:652–658, 1999. © 1999 Wiley‐Liss, Inc.

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