Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice

The lethal action in Balb/c mice of 80 oral Fusobacterium nucleatum recovered from patients with adult periodontitis, healthy subjects or Cebus apella monkeys was studied. Mice were inoculated intraperitoneally with each bacterial inoculum of 5 x 108 CFU/ml. All the clinical isolates induced weight and coordinated movements loss. Pathological alterations in liver, CNS, heart, and kidney with inflammatory reactions or vascular congestion were observed. Of all the tested F. nucleatum isolates, 61.2% from periodontal patients, 57.1% from healthy subjects and 60% from monkeys, were capable of killing the mice in 48h. The clinical isolates were significantly more pathogenic than F. nucleatum ATCC 10953 or ATCC 25586. B. fragilis ATCC 23745 showed lethality against control mice. Our results suggest that LPS could be involved in lethal action against mice and it may play an important role in producing tissue damage or death of mice.

[1]  M. J. Ávila-Campos,et al.  Oral species of Fusobacterium from human and environmental samples. , 1996, Journal of dentistry.

[2]  L. Kesavalu,et al.  Comparative virulence of periodontopathogens in a mouse abscess model. , 2008, Oral diseases.

[3]  W E Moore,et al.  The bacteria of periodontal diseases. , 1994, Periodontology 2000.

[4]  S. Socransky,et al.  Microbial etiological agents of destructive periodontal diseases. , 1994, Periodontology 2000.

[5]  C. Genco,et al.  Animal chamber models for study of host-parasite interactions. , 1994, Methods in enzymology.

[6]  J. Caton,et al.  Animal models for periodontal disease. , 1994, Methods in enzymology.

[7]  S. Schou,et al.  Non-human primates used in studies of periodontal disease pathogenesis: a review of the literature. , 1993, Journal of periodontology.

[8]  J. Baumgartner,et al.  Experimentally induced infection by oral anaerobic microorganisms in a mouse model. , 1992, Oral microbiology and immunology.

[9]  R. S. Tuttle,et al.  A non-lectin-like mechanism by which Fusobacterium nucleatum 10953 adheres to and activates human lymphocytes. , 1992, Oral microbiology and immunology.

[10]  D. I. Hay,et al.  Adhesive properties of strains of Fusobacterium nucleatum of the subspecies nucleatum, vincentii and polymorphum. , 1991, Oral microbiology and immunology.

[11]  P. Bartold,et al.  Identification of components in Fusobacterium nucleatum chemostat-culture supernatants that are potent inhibitors of human gingival fibroblast proliferation. , 1991, Journal of periodontal research.

[12]  T. Kirikae,et al.  Local skin response in mice induced by a single intradermal injection of bacterial lipopolysaccharide and lipid A , 1991, Infection and immunity.

[13]  M. B. Edlund,et al.  The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. , 1990, Oral microbiology and immunology.

[14]  M. B. Edlund,et al.  Production of volatile sulfur compounds by various Fusobacterium species. , 1990, Oral microbiology and immunology.

[15]  Y. Miyake,et al.  Binding specificity of Fusobacterium nucleatum to human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and HeLa cells. , 1990, Journal of periodontal research.

[16]  H. Nakamura,et al.  Cytotoxicity against various cell lines of lipopolysaccharides purified from Bacteroides, Fusobacterium, and Veillonella isolated from infected root canals. , 1989, Journal of endodontics.

[17]  W. Mcarthur,et al.  Natural occurrence of black-pigmented Bacteroides species in the gingival crevice of the squirrel monkey , 1988, Infection and immunity.

[18]  S. Holt,et al.  Implantation of Bacteroides gingivalis in nonhuman primates initiates progression of periodontitis. , 1988, Science.

[19]  J. Lewis,et al.  Factors affecting antimicrobial susceptibility of Fusobacterium species , 1987, Journal of clinical microbiology.

[20]  I. Brook,et al.  The relationship between Fusobacterium species and other flora in mixed infection. , 1986, Journal of medical microbiology.

[21]  B. Duerden,et al.  Identification of fusobacteria in a routine diagnostic laboratory. , 1985, The Journal of applied bacteriology.

[22]  W. Falkler,et al.  Haemolysis of human erythrocytes by the Fusobacterium nucleatum associated with periodontal disease. , 1983, Archives of oral biology.

[23]  W. Falkler,et al.  Attachment of cell fragments of Fusobacterium nucleatum to oral epithelial cells, gingival fibroblasts and white blood cells. , 1982, Archives of oral biology.

[24]  A. Willis.  Anaerobic Bacteriology Manual , 1981 .

[25]  J. Rochford.  Pleuropulmonary Infection Associated with Eubacterium brachy, a New Species of Eubacterium , 1980, Journal of clinical microbiology.

[26]  D. Citron,et al.  Wadsworth Anaerobic Bacteriology Manual , 1980 .

[27]  H. Nygren,et al.  Human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula , 1979, Infection and immunity.

[28]  M. Eckerbom,et al.  Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections , 1979, Infection and immunity.

[29]  S. Socransky,et al.  Histological changes in experimental periodontal disease in rats monoinfected with gram-negative organisms. , 1978, Journal of periodontal research.

[30]  K. Sveen.  Rabbit polymorphonuclear leukocyte migration in vitro in response to lipopolysaccharides from Bacteroides, Fusobacterium and Veillonella. , 2009, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[31]  K. Sveen,et al.  The capacity of lipopolysaccharides from bacteroides, fusobacterium and veillonella to produce skin inflammation and the local and generalized Shwartzman reaction in rabbits. , 1977, Journal of periodontal research.

[32]  W. Falkler,et al.  Hemagglutinating activity of Fusobacterium nucleatum , 1977, Infection and immunity.

[33]  S. Finegold.  Anaerobic bacteria in human disease , 1977 .

[34]  J. W. Holland,et al.  Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity , 1976, Infection and immunity.

[35]  G. Hill,et al.  Liver Abscess Production by Non-Spore-Forming Anaerobic Bacteria in a Mouse Model , 1974, Infection and immunity.