A recent development in image analysis of electrophoresis gels

Electrophoresis is an electrochemical separation process in which molecules, such as protein or RNA/DNA fragments, are made to migrate through a specific substrate, such as a polyacrylamide gel, under the influence of an electric current. The technique has a wide range of applications, in DNA sequencing and in studying variation in the identity and amount of proteins obtained from different sources. Techniques of image analysis and pattern recognition can be used to extract qualitative as well as quantitative information from the images, and spare human beings from voluminous, tedious image interpretation. More importantly, computerized data handling and interpretation provide accuracy and rapid speed without human errors. Here, we report the application of a newly developed system to the analysis of biological specimens that have undergone gel electrophoresis. The result of this application shows the capability to identify unique banding patterns of cDNA profiles, which paves the way for future full-scale investigation in the use of pattern recognition principles in biomedical information handling and interpretation.

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