Human immunodeficiency virus-1 infection of the human promyelocytic cell line HL-60: high frequency of low-level infection and effect of subsequent cell differentiation.

As a model system to study the infection of early myeloid cells by human immunodeficiency virus-1 (HIV-1), we have infected the human promyelocytic cell line, HL-60, with a recombinant selectable HIV-1 clone. A fully infected population showed a relatively high frequency of low-level infection, with 40% of subcloned cells being negative by reverse transcriptase and p24 indirect immunofluorescence analysis and displaying only low levels of supernatant p24. The same treatment of a T-lymphoid cell line produced 100% productive infections. HIV-1 infection of HL-60 did not appear to alter the state of differentiation of the cells, as assessed by surface antigen expression, regardless of the level of viral expression. Furthermore, infected cells were able to respond normally to chemical inducers of differentiation. Induction of differentiation towards monocyte/macrophages by phorbol myristate acetate activated the HIV-1 long terminal repeat in a transient transfection system, and there was a corresponding increase in viral production from the infected subclones. Granulocytic differentiation, as stimulated by dimethyl sulfoxide or retinoic acid, had no effect on long terminal repeat activity and did not stimulate viral replication. These data suggest that low-level HIV-1 infections may be established at a relatively high frequency in myeloid precursor cells, and that different pathways of promyelocytic differentiation vary in their ability to stimulate HIV-1 replication.

[1]  C. Crumpacker,et al.  In vitro suppression of normal human bone marrow progenitor cells by human immunodeficiency virus , 1991, Journal of virology.

[2]  R. Redfield,et al.  Induction of human immunodeficiency virus type 1 expression in chronically infected cells is associated primarily with a shift in RNA splicing patterns , 1991, Journal of virology.

[3]  北野 喜良 Differentiating agents facilitate infection of myeloid leukemia cell lines by monocytotropic HIV-1 strains , 1991 .

[4]  U. Testa,et al.  Increased human immunodeficiency virus (HIV) expression in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3: roles of interferon and tumor necrosis factor in regulation of HIV production , 1991, Journal of virology.

[5]  D. Baltimore,et al.  A human cell factor is essential for HIV‐1 Rev action. , 1990, The EMBO journal.

[6]  H. Kessler,et al.  Analysis of lymphocytes, monocytes, and neutrophils from human immunodeficiency virus (HIV)-infected persons for HIV DNA. , 1990, The Journal of infectious diseases.

[7]  D. Baltimore,et al.  Factors affecting cellular tropism of human immunodeficiency virus , 1990, Journal of virology.

[8]  A. Valentin,et al.  Characterization of the in vitro maturation of monocytes and the susceptibility to HIV infection. , 1990, AIDS research and human retroviruses.

[9]  J. Haas,et al.  Infection of the human monocytic cell line Mono Mac6 with human immunodeficiency virus types 1 and 2 results in long-term production of virus variants with increased cytopathogenicity for CD4+ T cells , 1990, Journal of virology.

[10]  J. McCutchan,et al.  T-cell-induced expression of human immunodeficiency virus in macrophages , 1990, Journal of virology.

[11]  D. Baltimore,et al.  Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latency , 1990, Cell.

[12]  M. Martin,et al.  Replication of HIV-1 in primary monocyte cultures. , 1990, Virology.

[13]  D. Mann,et al.  HIV-1 transmission and function of virus-infected monocytes/macrophages. , 1990, Journal of immunology.

[14]  G. Nabel,et al.  An inducible transcription factor activates expression of human immunodeficiency virus in T cells , 1990, Nature.

[15]  R. Walker,et al.  Infection of monocyte-derived macrophages with human immunodeficiency virus type 1 (HIV-1). Monocyte-tropic and lymphocyte-tropic strains of HIV-1 show distinctive patterns of replication in a panel of cell types , 1989, The Journal of experimental medicine.

[16]  D. Baltimore,et al.  Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression , 1989, Journal of virology.

[17]  H. Gendelman,et al.  The macrophage in the persistence and pathogenesis of HIV infection. , 1989, AIDS.

[18]  C H Fox,et al.  The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4. , 1989, Science.

[19]  G. Nabel,et al.  Activation of HIV gene expression during monocyte differentiation by induction of NF-kB , 1989, Nature.

[20]  G. Frederickson,et al.  L3T4 antigen expression by hemopoietic precursor cells , 1989, The Journal of experimental medicine.

[21]  A. Fauci,et al.  Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone. , 1989, Journal of immunology.

[22]  C. Smith,et al.  HIV-1-stimulated expression of CD11/CD18 integrins and ICAM-1: a possible mechanism for extravascular dissemination of HIV-1-infected cells. , 1989, Transactions of the Association of American Physicians.

[23]  D. Tenen,et al.  Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myeloid differentiation. , 1989, Blood.

[24]  M. McElrath,et al.  Mononuclear phagocytes of blood and bone marrow: comparative roles as viral reservoirs in human immunodeficiency virus type 1 infections. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[25]  M. Cairo,et al.  Impaired neutrophil function in patients with AIDS or AIDS-related complex: a comprehensive evaluation. , 1988, The Journal of infectious diseases.

[26]  E. Jaffe,et al.  Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow , 1988, Science.

[27]  D. Richman,et al.  Human immunodeficiency virus infection of monoblastoid cells: cellular differentiation determines the pattern of virus replication , 1988, Journal of virology.

[28]  A. Fauci,et al.  Impairment of neutrophil bactericidal capacity in patients with AIDS. , 1988, The Journal of infectious diseases.

[29]  I. Weissman,et al.  Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus , 1988, Cell.

[30]  D. Burke,et al.  Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes , 1988, The Journal of experimental medicine.

[31]  J. Justement,et al.  Characterization of a promonocyte clone chronically infected with HIV and inducible by 13-phorbol-12-myristate acetate. , 1988, Journal of immunology.

[32]  J. Justement,et al.  Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line. , 1987, Science.

[33]  S. Collins,et al.  The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression. , 1987, Blood.

[34]  M. Popovič,et al.  ISOLATION OF HIV-1 FROM MONOCYTES BUT NOT T LYMPHOCYTES , 1987, The Lancet.

[35]  F. Miedema,et al.  Human immunodeficiency virus infection down-regulates HLA class II expression and induces differentiation in promonocytic U937 cells. , 1987, The Journal of clinical investigation.

[36]  H. Vinters,et al.  Dual infection of the central nervous system by AIDS viruses with distinct cellular tropisms. , 1987, Science.

[37]  N. Hogg,et al.  Human immunodeficiency virus infection of monocytic and T-lymphocytic cells: receptor modulation and differentiation induced by phorbol ester. , 1987, Virology.

[38]  J. Groopman,et al.  Suppression of in vitro haematopoiesis following human immunodeficiency virus infection , 1987, Nature.

[39]  N. Yamamoto,et al.  Tumor promoter, TPA, enhances replication of HTLV-III/LAV. , 1986, Virology.

[40]  D. Markovitz,et al.  The role of mononuclear phagocytes in HTLV-III/LAV infection. , 1986, Science.

[41]  J. Mcdougal,et al.  In vitro infection of human monocytes with human T lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). , 1986, Journal of immunology.

[42]  H. Gendelman,et al.  Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages , 1986, Journal of virology.

[43]  M. Gonda,et al.  The trans-activator gene of HTLV-III is essential for virus replication , 1986, Nature.

[44]  J. Mullins,et al.  Disease-specific and tissue-specific production of unintegrated feline leukaemia virus variant DNA in feline AIDS , 1986, Nature.

[45]  J. Levy,et al.  Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. , 1985, Virology.

[46]  J. Levy,et al.  AIDS-associated retroviruses (ARV) can productively infect other cells besides human T helper cells. , 1985, Virology.

[47]  H. Gendelman,et al.  Slow, persistent replication of lentiviruses: role of tissue macrophages and macrophage precursors in bone marrow. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[48]  J. Sodroski,et al.  Location of cis-acting regulatory sequences in the human T-cell leukemia virus type I long terminal repeat. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[49]  R. Grosschedl,et al.  Cell-type specificity of iminunoglobulin gene expression is regulated by at least three DNA sequence elements , 1985, Cell.

[50]  J. Sodroski,et al.  The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat , 1985, Cell.

[51]  M. Greaves,et al.  The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus , 1984, Nature.

[52]  R. Schooley,et al.  HTLV-III in the semen and blood of a healthy homosexual man. , 1984, Science.

[53]  R. Graziano,et al.  The expression and modulation of human myeloid-specific antigens during differentiation of the HL-60 cell line. , 1983, Blood.

[54]  B. Howard,et al.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells , 1982, Molecular and cellular biology.

[55]  H. Varmus,et al.  5 Replication of Retroviruses , 1982 .

[56]  John D. Minna,et al.  Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma , 1980, Proceedings of the National Academy of Sciences.

[57]  S. Collins,et al.  Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. , 1979, Blood.

[58]  S. Collins,et al.  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture , 1977, Nature.