Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt's lymphoma.

A patient with Burkitt's lymphoma in complete remission received myeloablative consolidation treatment with superfractionated total body irradation (1,320 rad) and cyclophosphamide (200 mg/kg) followed by autologous transplantation of previously harvested and cryopreserved blood-derived hemopoietic stem cells. Seven successive leukaphereses were performed to yield a total of 55.2 X 10(9) mononuclear cells (MNC) comprising 15.1 X 10(6) CFU-GM or 4.34 X 10(6) CFU-GEMM. Following autologous blood stem cell transplantation (ABSCT), reconstitution of all cell lines occurred very rapidly, ie, 1,000 leucocytes per microL were reached after nine days, 500 granulocytes and 50,000 platelets per microL after ten days. B cells reached normal values around day 35 post-transplantation. CFU-GM first appeared in the circulating blood exhibiting an enormous overshoot. Some days later CFU-GM also appeared in the marrow. The kinetics and pattern of hemopoietic reconstitution after myeloablative treatment and ABSCT provide clear evidence that blood-derived hemopoietic stem cells are capable of completely restoring hemopoietic function in man. A possible reconstitutive advantage of blood over marrow-derived stem cells is discussed.

[1]  E. Thiel,et al.  HD37 Monoclonal Antibody: A Useful Reagent for Further Characterization of “Non-T, Non-B” Lymphoid Malignancies , 1986 .

[2]  T. Fliedner,et al.  Stem cells from peripheral blood and bone marrow: a comparative evaluation of the hemopoietic potential in the dog. , 1983, International journal of cell cloning.

[3]  E. Zanjani,et al.  Studies of human pluripotential hemopoietic stem cells (CFU-GEMM) in vitro. , 1981, Blood.

[4]  J. Hansen,et al.  Fatal graft-versus-host disease in a patient with lymphoblastic leukemia following normal granulocyte transfusion. , 1981, Blood.

[5]  T. Fliedner,et al.  Collection of large quantities of granulocyte/macrophage progenitor cells (CFUc) in man by means of continuous-flow leukapheresis. , 2009, Scandinavian journal of haematology.

[6]  Appelbaum Fr Hemopoietic reconstitution following autologous bone marrow and peripheral blood mononuclear cell infusions. , 1979 .

[7]  R. Gale,et al.  Isolation, cryopreservation, and autotransplantation of human stem cells. , 1979, Experimental hematology.

[8]  R. Storb,et al.  Marrow transplantation for treatment of aplastic anaemia. , 1978, Clinics in haematology.

[9]  W. Nothdurft,et al.  Studies on the regeneration of the CFU-C population in blood and bone marrow of lethally irradiated dogs after autologous transfusion of cryopreserved mononuclear blood cells. , 2009, Scandinavian journal of haematology.

[10]  R. Storb,et al.  Demonstration of hemopoietic stem cells in the peripheral blood of baboons by cross circulation. , 1977, Blood.

[11]  H. Flad,et al.  The repopulation of lymph nodes of dogs after 1200 R whole-body x-irradiation and intravenous administration of mononuclear blood leukocytes. , 1976, The American journal of pathology.

[12]  R. Weiner,et al.  Increase in Circulating Stem Cells Following Chemotherapy in Man , 1976 .

[13]  R. Weiner,et al.  Increase in circulating stem cells following chemotherapy in man. , 1976, Blood.

[14]  H. Micklem,et al.  Limited potential of circulating haemopoietic stem cells , 1975, Nature.

[15]  E. Thomas,et al.  THE RECOVERY OF LETHALLY IRRADIATED DOGS GIVEN INFUSIONS OF AUTOLOGOUS LEUKOCYTES PRESERVED AT -80 C. , 1964, Blood.