Phytochemical Composition and Antibacterial Activity of Brown Seaweed, Padina australis against Human Pathogenic Bacteria

Seaweeds have become targets for chemical screening in search of new compounds that have potential medical value, including antibacterial activity. The aim of this study was to evaluate the chemical composition and antibacterial activity of the extract and fractions of Padina australis . The chemical compounds in seaweed samples were extracted with methanol, then sequentially partitioned with hexane, dichloromethane, ethyl acetate (EA) and n-butanol. The EA fraction was further separated using silica gel column chromatography to yield eight subfractions (EA-1 to EA-8). The antibacterial activity was tested against seven pathogenic bacteria through the disc diffusion method and its minimum inhibitory concentration was tested using the standard broth dilution method. The highest activity against gram-positive bacteria was observed for the EA-1 subfraction with inhibition zones of 9.37±0.09 mm, 12.25±0.60 mm and 10.30±0.05 mm for Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923 and methicillin-resistant S. aureus KL046, respectively. None of the extracts showed activity against gram-negative bacteria. Moreover, the antibacterial activity was positively correlated with the total phenolic content in the extracts against B. subtilis (r = 0.656), S. aureus (r = 0.800) and methicillin-resistant S. aureus (r = 0.880). The highest fucoxanthin content was also observed in the EA-1 subfraction. The active compounds of the EA-1 subfraction were identified using gas chromatography coupled with mass spectroscopy, and the major components were fucosterol, (3β, 24Z)-Stigmasta-5, 24(28)-dien-3-ol and phloroglucinol. Based on these results, the bioactive compounds responsible for the antibacterial activity might be the phenolic compounds fucoxanthin, fucosterol and fucosterol derivative

[1]  F. Madkour,et al.  Antibacterial activity of some seaweeds from the Red Sea coast of Egypt. , 2019, Egyptian Journal of Aquatic Biology and Fisheries.

[2]  Jayzon G Bitacura,et al.  Cytotoxicity and Antiangiogenic Activity of Turbinaria ornata Agardh and Padina australis Hauck Ethanolic Extracts , 2018, Analytical cellular pathology.

[3]  K. Miyashita,et al.  Seasonal variation in nutritional composition and anti-proliferative activity of brown seaweed, Sargassum oligocystum , 2018, Journal of Applied Phycology.

[4]  Mehdi Dridi,et al.  Phytochemical Analysis and Evaluation of the Antioxidant, Anti-Inflammatory, and Antinociceptive Potential of Phlorotannin-Rich Fractions from Three Mediterranean Brown Seaweeds , 2018, Marine Biotechnology.

[5]  S. Jeeva,et al.  Antioxidant properties of phlorotannins from brown seaweed Cystoseira trinodis (Forsskål) C. Agardh , 2017 .

[6]  M. Yousefzadi,et al.  Cytotoxicity and antimicrobial activity of marine macro algae (Dictyotaceae and Ulvaceae) from the Persian Gulf , 2016, Cytotechnology.

[7]  P. Anantharaman,et al.  Antifouling Activity of Lipidic Metabolites Derived from Padina tetrastromatica , 2016, Applied Biochemistry and Biotechnology.

[8]  M. El-sheekh,et al.  Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria , 2016 .

[9]  A. Woźniak,et al.  The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts , 2016, Journal of analytical methods in chemistry.

[10]  A. Boonyasiri,et al.  Thailand Antimicrobial Resistance Containment and Prevention Program. , 2015, Journal of global antimicrobial resistance.

[11]  S. Benjakul,et al.  Extraction, antioxidative, and antimicrobial activities of brown seaweed extracts, Turbinaria ornata and Sargassum polycystum, grown in Thailand , 2015, International Aquatic Research.

[12]  M. Jahangir,et al.  Antioxidant evaluations of polar and non-polar fractions of Cajanus cajan seeds , 2015 .

[13]  A. Horta,et al.  Antitumor and Antimicrobial Potential of Bromoditerpenes Isolated from the Red Alga, Sphaerococcus coronopifolius , 2015, Marine drugs.

[14]  R. Moo-Puc,et al.  Cytotoxic and antiproliferative constituents from Dictyota ciliolata, Padina sanctae-crucis and Turbinaria tricostata , 2014, Pharmaceutical biology.

[15]  G. Fonseca-Camarillo,et al.  Expression of interleukin (IL)‐19 and IL‐24 in inflammatory bowel disease patients: a cross‐sectional study , 2014, Clinical and experimental immunology.

[16]  Deivasigamani Balaraman,et al.  Screening of marine seaweeds for bioactive compound against fish pathogenic bacteria and active fraction analysed by gas chromatography- mass spectrometry , 2014 .

[17]  B. Jha,et al.  24-Branched Δ5 sterols from Laurencia papillosa red seaweed with antibacterial activity against human pathogenic bacteria. , 2014, Microbiological research.

[18]  Hyo Jung Kim,et al.  In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria , 2014 .

[19]  C. Strain,et al.  Seaweed and human health. , 2014, Nutrition reviews.

[20]  Y. Jeon,et al.  Application of Yeast Candida utilis to Ferment Eisenia bicyclis for Enhanced Antibacterial Effect , 2013, Applied Biochemistry and Biotechnology.

[21]  G. Rajauria,et al.  Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed: A TLC-Based Approach , 2013, International journal of analytical chemistry.

[22]  S. Eom,et al.  Potential of Candida utilis to ferment Ecklonia cava by-product for enhanced anti-methicillin-resistant Staphylococcus aureus (MRSA) activity , 2013, Journal of Applied Phycology.

[23]  S. Eom,et al.  Antimicrobial effect of phlorotannins from marine brown algae. , 2012, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[24]  Y. Jeon,et al.  Phlorotannins of the edible brown seaweed Ecklonia cava Kjellman induce sleep via positive allosteric modulation of gamma-aminobutyric acid type A-benzodiazepine receptor: A novel neurological activity of seaweed polyphenols. , 2012, Food chemistry.

[25]  T. Shin,et al.  Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and hepatoprotective properties in tacrine-treated HepG2 cells. , 2012, Journal of agricultural and food chemistry.

[26]  S. Eom,et al.  Antimicrobial Activity of Brown Alga Eisenia bicyclis against Methicillin-resistant Staphylococcus aureus , 2011 .

[27]  H. Salleh,et al.  Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines , 2011 .

[28]  Se-kwon Kim,et al.  Phlorotannins as bioactive agents from brown algae , 2011 .

[29]  S. Sitthisak,et al.  Prevalence of methicillin-resistant Staphylococcus aureus among university students in Thailand. , 2011, The Southeast Asian journal of tropical medicine and public health.

[30]  Chong Chiao-Wei,et al.  Antibacterial activity of Sargassum polycystum C. Agardh and Padina australis Hauck (Phaeophyceae) , 2011 .

[31]  Juan Peng,et al.  Fucoxanthin, a Marine Carotenoid Present in Brown Seaweeds and Diatoms: Metabolism and Bioactivities Relevant to Human Health , 2011, Marine drugs.

[32]  M. Provinciali,et al.  Vitamin E supplementation in old mice induces antimicrobial activity and improves the efficacy of daptomycin in an animal model of wounds infected with methicillin-resistant Staphylococcus aureus. , 2011, The Journal of antimicrobial chemotherapy.

[33]  N. Abu-Ghannam,et al.  Bioactive potential and possible health effects of edible brown seaweeds , 2011 .

[34]  Aroa López,et al.  The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulon scoparium algae extracts , 2011 .

[35]  J. Pekkoh,et al.  Antimicrobial and anti-inflammatory properties of various seaweeds from the gulf of Thailand , 2011 .

[36]  V. Gupta,et al.  New antifungal steroids from Turbinaria conoides (J. Agardh) Kutzing , 2010, Natural product research.

[37]  C. Tribuddharat,et al.  MRSA carriage in a tertiary governmental hospital in Thailand: emphasis on prevalence and molecular epidemiology , 2010, European Journal of Clinical Microbiology & Infectious Diseases.

[38]  Jang-Gi Choi,et al.  Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp. , 2010, Foodborne pathogens and disease.

[39]  Zhong‐Ji Qian,et al.  Chemical components and its antioxidant properties in vitro: an edible marine brown alga, Ecklonia cava. , 2009, Bioorganic & medicinal chemistry.

[40]  M. Heo,et al.  Antioxidant and antimicrobial activities of seaweed, Ecklonia cava , 2008 .

[41]  J. Santoso,et al.  Effects of Extracts from Tropical Seaweeds on DPPH Radicals and Caco-2 Cells Treated with Hydrogen Peroxide , 2007 .

[42]  A. J. Smit.  Medicinal and pharmaceutical uses of seaweed natural products: A review , 2004, Journal of Applied Phycology.

[43]  Toshiyuki Shibata,et al.  Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. , 2002, The Journal of antimicrobial chemotherapy.

[44]  S. Warber,et al.  Natural Products from Plants , 1998 .

[45]  A. Rizk,et al.  Major sterol composition of some algae from Qatar , 1995 .

[46]  A. Samb,et al.  Fatty acid and sterol composition of eight brown algae from the Senegalese coast , 1992 .

[47]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[48]  Indriatmoko,et al.  Composition of the Main Dominant Pigments from Potential Two Edible Seaweeds , 2018 .

[49]  M. Maheswari,et al.  GC-MS ANALYSIS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF THE BROWN ALGAE, PADINA TETRASTROMATICA , 2017 .

[50]  A. Amin,et al.  Antimicrobial Stewardship: Hospital Strategies to Curb Antibiotic Resistance , 2016 .

[51]  K. Zailanie.  Study of Padina australis using UV-VIS, HPLC and Antibacterial. J. Life Sci. Biomed. 6(1): 01-05 , 2016 .

[52]  Mahboba B. Naili,et al.  In vitro antibacterial activity of alkaloid extracts from green , red and brown macroalgae from western coast of Libya , 2013 .

[53]  A. Prathep,et al.  Diversity and distribution of seaweed at Khanom-Mu Ko Thale Tai National Park , Nakhon Si Thammarat Province , Thailand , 2012 .

[54]  Se-kwon Kim,et al.  Potential beneficial effects of marine algal sterols on human health. , 2011, Advances in food and nutrition research.

[55]  K. Miyashita,et al.  Comparative antioxidant activity of edible Japanese brown seaweeds. , 2011, Journal of food science.

[56]  A. Chirapart,et al.  Components and Antimicrobial Activity of Polysaccharides Extracted from Thai Brown Seaweeds , 2010 .

[57]  N. Abu-Ghannam,et al.  An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds , 2010 .

[58]  K. Stefanova,et al.  Chemical Composition of the Brown Alga Padina pavonia ( L . ) Gaill . from the Adriatic Sea , 2004 .

[59]  Mary Jane Ferraro,et al.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard , 2000 .