Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor.

Voltammetric enzyme dual sensors for simultaneous determination of free and total prostate specific antigen (fPSA and tPSA) are described. Alkaline Phosphatase (AP) and a mixture solution of 3-indoxyl phosphate and silver ions were used as the enzymatic label and substrate, respectively. 8A6 or 5G6 antibodies specific for free and total PSA, respectively, were immobilized on different screen-printed electrodes (SPEs)--screen-printed carbon electrodes, screen-printed gold electrodes and screen-printed carbon electrodes modified with nanogold--in order to be able to select one of the surfaces as the most adequate one to develop the dual sensor. Screen-printed carbon electrodes modified with nanogold were the SPEs with the best analytical characteristics and lead to the most repeatable bioelectrodes, so they were selected for the development of the dual sensor. On Dualsensor-nAu electrodes, 8A6 antibody was immobilized on one working electrode and 5G6 antibody was immobilized on the other one by deposition of a drop of solution of each antibody and left overnight at 4 degrees C. Biotinylated anti-PSA antibody and streptavidin-AP conjugate were used as detection reagents, giving rise, to our knowledge, to the first simultaneous electrochemical biosensor for free and total PSA. The PSA dual sensor was used to monitor PSA production from three different cultures of human androgen-sensitive prostate tumor cells.

[1]  O. Nilsson,et al.  Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin. , 1991, Clinical chemistry.

[2]  Julio Raba,et al.  Integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (PSA) in human serum samples. , 2008, Biosensors & bioelectronics.

[3]  César Fernández-Sánchez,et al.  Disposable noncompetitive immunosensor for free and total prostate-specific antigen based on capacitance measurement. , 2004, Analytical chemistry.

[4]  M. Porter,et al.  Femtomolar detection of prostate-specific antigen: an immunoassay based on surface-enhanced Raman scattering and immunogold labels. , 2003, Analytical chemistry.

[5]  P. O’Neill,et al.  Use of an optical biosensor to measure prostate-specific antigen in whole blood , 1995 .

[6]  Sang Jun Sim,et al.  A strategy for sensitivity and specificity enhancements in prostate specific antigen-alpha1-antichymotrypsin detection based on surface plasmon resonance. , 2006, Biosensors & bioelectronics.

[7]  Dipankar Ghosh,et al.  Amperometric biosensors for detection of the prostate cancer marker (PSA). , 2002, International journal of pharmaceutics.

[8]  César Fernández-Sánchez,et al.  Quantitative impedimetric immunosensor for free and total prostate specific antigen based on a lateral flow assay format , 2004 .

[9]  Shusheng Zhang,et al.  Amperometric Immunosensor for Prostate Specific Antigen Based on Co-adsorption of Labeled Antibody and Mediator in Nano-Au Modified Chitosan Membrane , 2008 .

[10]  E. Tamiya,et al.  Label-free immunosensor for prostate-specific antigen based on single-walled carbon nanotube array-modified microelectrodes. , 2007, Biosensors & bioelectronics.

[11]  Yan Liu Electrochemical detection of prostate-specific antigen based on gold colloids/alumina derived sol-gel film , 2008 .

[12]  César Fernández-Sánchez,et al.  One-step immunostrip test for the simultaneous detection of free and total prostate specific antigen in serum. , 2005, JIM - Journal of Immunological Methods.

[13]  Guodong Liu,et al.  Disposable electrochemical immunosensor diagnosis device based on nanoparticle probe and immunochromatographic strip. , 2007, Analytical chemistry.

[14]  Y. Xu,et al.  Electrochemical enzyme immunoassay for serum prostate-specific antigen at low concentrations. , 1997, Clinical chemistry.

[15]  Itamar Willner,et al.  Inhibition of the acetycholine esterase-stimulated growth of Au nanoparticles: nanotechnology-based sensing of nerve gases. , 2005, Nano letters.

[16]  Agustín Costa-García,et al.  Alkaline phosphatase-catalyzed silver deposition for electrochemical detection. , 2007, Analytical chemistry.

[17]  T. Thundat,et al.  Bioassay of prostate-specific antigen (PSA) using microcantilevers , 2001, Nature Biotechnology.

[18]  Tatsuro Endo,et al.  Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopy. , 2007, Talanta.

[19]  M. Satoh,et al.  Enzyme-linked immunosorbent assay detection of prostate-specific antigen messenger ribonucleic acid in prostate cancer. , 1999, Urology.

[20]  G. Murphy,et al.  Quantitation of prostate-specific antigen in serum by a sensitive enzyme immunoassay. , 1980, Cancer research.

[21]  Tae Song Kim,et al.  Immunoassay of prostate-specific antigen (PSA) using resonant frequency shift of piezoelectric nanomechanical microcantilever. , 2005, Biosensors & bioelectronics.

[22]  Dipankar Ghosh,et al.  Electrochemical Immunoassay for Free Prostate Specific Antigen (f-PSA) Using Magnetic Beads , 2008 .

[23]  Shusheng Zhang,et al.  Detection of prostate specific antigen with 3,4-diaminobenzoic acid (DBA)-H(2)O(2)-HRP voltammetric enzyme-linked immunoassay system. , 2007, Talanta.

[24]  Paul Leonard,et al.  Biosensor developments: application to prostate-specific antigen detection. , 2007, Trends in biotechnology.

[25]  Xue Zhang,et al.  A novel multi-array immunoassay device for tumor markers based on insert-plug model of piezoelectric immunosensor. , 2007, Biosensors & bioelectronics.

[26]  Yanjun Ding,et al.  Cell-based immobilization strategy for sensitive piezoelectric immunoassay of total prostate specific antigen. , 2008, Biosensors & bioelectronics.

[27]  Yafeng Wu,et al.  Prostate-specific antigen detection by using a reusable amperometric immunosensor based on reversible binding and leasing of HRP-anti-PSA from phenylboronic acid modified electrode. , 2008, Clinica chimica acta; international journal of clinical chemistry.

[28]  J. Hainfeld,et al.  Enzymatic control of metal deposition as key step for a low-background electrical detection for DNA chips. , 2005, Nano letters.

[29]  P. Yáñez‐Sedeño,et al.  Gold nanoparticle-based electrochemical biosensors , 2005, Analytical and bioanalytical chemistry.

[30]  Tatsuro Endo,et al.  Gold nanoparticle based immunochromatography using a resin modified micropipette tip for rapid and simple detection of human chorionic gonadotropin hormone and prostate-specific antigen , 2006 .