Regulation of c-fos expression in adipose and muscle tissue of diabetic rats.

Insulin treatment of control rats demonstrated a marked 8-fold transient increase (15 min) in c-fos mRNA in white adipose tissue, which returns to basal levels by 5 h. Similarly, insulin treatment resulted in a rapid 9-fold increase in cardiac muscle c-fos mRNA, which also returned to control values by 1 h. By contrast, insulin treatment resulted in only a small increase in c-jun mRNA levels in both adipose tissue and cardiac muscle. Similarly, the expression of c-jun mRNA was only slightly responsive to insulin in these tissues from streptozocin-induced insulin-deficient diabetic rats. However, insulin treatment of insulin-deficient diabetic rats resulted in a prolonged increase in c-fos message levels in adipose tissue without any significant change in the time course of c-fos mRNA induction/repression in cardiac muscle. These data demonstrate that in contrast to c-jun, c-fos is transiently increased in both cardiac muscle and adipose tissue by insulin treatment. Furthermore, transrepression of the c-fos gene is specifically attenuated in adipose tissue of insulin-deficient diabetic rats, but not in cardiac muscle.

[1]  Michael E. Greenberg,et al.  Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene , 1984, Nature.

[2]  T. Curran,et al.  Isolation and characterization of the c-fos(rat) cDNA and analysis of post-translational modification in vitro. , 1987, Oncogene.

[3]  W. Pledger,et al.  Platelet-derived growth factor regulation of fos stability correlates with growth induction. , 1992, The Journal of biological chemistry.

[4]  J. Woodgett,et al.  Phorbol ester-induced amino-terminal phosphorylation of human JUN but not JUNB regulates transcriptional activation. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[5]  T. Curran,et al.  Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini , 1983, Cell.

[6]  I. Verma,et al.  Transcriptional autoregulation of the proto-oncogene fos , 1988, Nature.

[7]  J. Pessin,et al.  Regulation of the GLUT4/muscle-fat glucose transporter mRNA in adipose tissue of insulin-deficient diabetic rats. , 1993, The Journal of biological chemistry.

[8]  J. L. Messina,et al.  Regulation of β-actin gene transcription by insulin and phorbol esters , 1992 .

[9]  I. Simpson,et al.  Divergent mechanisms for the insulin resistant and hyperresponsive glucose transport in adipose cells from fasted and refed rats. Alterations in both glucose transporter number and intrinsic activity. , 1988, The Journal of clinical investigation.

[10]  D. Stumpo,et al.  Insulin and growth factor effects on c-fos expression in normal and protein kinase C-deficient 3T3-L1 fibroblasts and adipocytes. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[11]  S. Perlman,et al.  Developmental Regulation of Angiotensinogen Gene Expression in Sheep , 1990, Pediatric Research.

[12]  Jonathan A. Cooper,et al.  Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein , 1984, Nature.

[13]  T. Hunter,et al.  The c-fos protein interacts with c-Jun AP-1 to stimulate transcription of AP-1 responsive genes , 1988, Cell.

[14]  M. Quon,et al.  Postbinding characterization of five naturally occurring mutations in the human insulin receptor gene: impaired insulin-stimulated c-jun expression and thymidine incorporation despite normal receptor autophosphorylation. , 1992, Biochemistry.

[15]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[16]  G. Bell,et al.  Expression and regulation of the human GLUT4/muscle-fat facilitative glucose transporter gene in transgenic mice. , 1992, The Journal of biological chemistry.

[17]  P. Neufer,et al.  Transcriptional regulation of the gene for glucose transporter GLUT4 in skeletal muscle. Effects of diabetes and fasting. , 1993, The Journal of biological chemistry.

[18]  I. Verma,et al.  Induction of proto-oncogene JUN/AP-1 by serum and TPA , 1988, Nature.

[19]  R. Treisman,et al.  Fos C‐terminal mutations block down‐regulation of c‐fos transcription following serum stimulation. , 1988, The EMBO journal.

[20]  F. C. Lucibello,et al.  Trans-repression of the mouse c-fos promoter: A novel mechanism of fos-mediated trans-regulation , 1989, Cell.

[21]  J. L. Messina,et al.  Insulin's regulation of c-fos gene transcription in hepatoma cells. , 1990, The Journal of biological chemistry.

[22]  R. Taub,et al.  Insulin as a growth factor in rat hepatoma cells. Stimulation of proto-oncogene expression. , 1987, The Journal of biological chemistry.

[23]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[24]  A. Schönthal,et al.  Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters , 1988, Cell.