Study on the Susceptibility of Lncrna PCAT1 Snps and Breast Cancer Risk in the Chinese Population

The purpose of this study is to explore the relationship between PCAT1-SNPs and breast cancer (BC) susceptibility. Logistic regression analysis was applied to determine the association between PCAT1-SNPs and BC risk. The relative expression of PCAT1 in different genotypes was detected by qRT-PCR. The binding between the genotype of C/T at rs4473999 locus and miR-149-5p was confirmed by dual luciferase gene reporter assays. The proliferation, migration and invasion of BC cells with dysregulated expression of miR-149-5p was evaluated by CCK8, Scratch and Transwell assay, respectively. Logistic regression analysis revealed PCAT1-SNPs was related to the susceptibility of BC that rs117117537 (OR:2.413, 95%CI: 1.057–5.508) and rs4473999 (OR:2.137 95%CI: 1.065–4.286) were risk factors of BC when the menopausal age was ≥ 50; The haplotype Grs1551514Trs1551513Crs4473999Crs9656964Trs17762938Crs7823297Trs785003Trs117117537 may increase the risk of BC (OR:1.614 95%CI: 1.116–2.333), and there was an association between genes and reproductive factors (OR:2.487 95%CI: 1.929–3.206). Preliminary functional studies demonstrated that PCAT1 interacted with miR-149-5p when rs4473999 carried wild type C; In addition, the dysregulated enrichment of miR-149-5p may affect the proliferation, invasion and migration of BC cells. Our study shows that PCAT1 gene polymorphism is related to BC susceptibility, PCAT1-rs4473999 C/T genotype may affect the occurence of BC by modulating the interactions with miR-149-5p.