Histologic characteristics of breast cancers with occult lymph node metastases detected by keratin 19 mRNA reverse transcriptase‐polymerase chain reaction

Amplification of keratin 19 mRNA (K19) by reverse transcriptase‐polymerase chain reaction (RT‐PCR) has been shown to be a sensitive method to detect occult breast cancer metastases in lymph nodes.

[1]  M. Lagios,et al.  Duct carcinoma in situ. Relationship of extent of noninvasive disease to the frequency of occult invasion, multicentricity, lymph node metastases, and short‐term treatment failures , 1982 .

[2]  J. Coindre,et al.  Prognostic significance of breast cancer axillary lymph node micrometastases assessed by two special techniques: reevaluation with longer follow-up. , 1992, British Journal of Cancer.

[3]  J. Rossi,et al.  Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction. , 1991, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[4]  T. Aihara,et al.  Detection of Gastric Cancer Micrometastases in Lymph Nodes by Amplification of Keratin 19 mRNA with Reverse Transcriptase‐Polymerase Chain Reaction , 1996, Japanese journal of cancer research : Gann.

[5]  D. Kufe,et al.  Immunohistochemical detection and significance of axillary lymph node micrometastases in breast carcinoma. A study of 97 cases. , 1993, Analytical and quantitative cytology and histology.

[6]  S. Shousha,et al.  Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction. , 1994, Cancer research.

[7]  R. Hutter,et al.  Significance of Axillary Macrometastases and Micrometastases in Mammary Cancer , 1971, Annals of surgery.

[8]  Shinzaburo Noguchi,et al.  The detection of breast carcinoma micrometastases in axillary lymph nodes by means of reverse transcriptase‐polymerase chain reaction , 1994, Cancer.

[9]  S. Friedman,et al.  Importance of tumor cells in axillary node sinus margins ('clandestine' metastases) discovered by serial sectioning in operable breast carcinoma. , 1988, Acta oncologica.

[10]  S. T. Traweek,et al.  Keratin gene expression in non-epithelial tissues. Detection with polymerase chain reaction. , 1993, The American journal of pathology.

[11]  R. Coombes,et al.  Polymerase chain reaction-aided analysis of gene expression in frozen tissue sections. , 1992, Analytical biochemistry.

[12]  S. Ethier,et al.  Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction. , 1994, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[13]  S. Noguchi,et al.  Detection of breast cancer micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction. Comparison between MUC1 mRNA and keratin 19 mRNA amplification. , 1996, The American journal of pathology.

[14]  Benjamin Geiger,et al.  The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells , 1982, Cell.