Synthesis of NAD and catabolism of nicotinic acid in the isolated perfused liver of normal and X-irradiated rat.

SummaryThe synthesis of NAD and the formation of catabolites from nicotinic acid 14C was studied in isolated perfused liver from normal and x-irradiated rats. Large amounts of nicotinic acid and nicotinamide increase NAD content of perfused liver as they do in vivo. Synthesis of NAD is slightly diminished after irradiation, particularly under loading conditions whereas its catabolism is enhanced. Data for the rates of reactions are given for different pathways involved in NAD metabolism.

[1]  C. Streffer.  [The formation of nicotinamide-adenine-dinucleotide from tryptophan after whole-body irradiation]. , 1967, German medical monthly.

[2]  D. Abelson,et al.  Spectrophotometric determination of plasma pyridones in man following nicotinamide administration, with a general note on the quantitative treatment of interfering substances in analytical methods. , 1966, Clinica chimica acta; international journal of clinical chemistry.

[3]  H. Ijichi,et al.  Studies on the biosynthesis of nicotinamide adenine dinucleotide. 3. Comparative in vivo studies on nicotinic acid, nicotinamide, and quinolinic acid as precursors of nicotinamide adenine dinucleotide. , 1966, The Journal of biological chemistry.

[4]  P. Greengard,et al.  On the relative efficacy of nicotinamide and nicotinic acid as precursors of nicotinamide adenine dinucleotide. , 1966, The Journal of biological chemistry.

[5]  L. Carlson.  Determination of free nicotinic acid in blood plasma. , 1966, Clinica chimica acta; international journal of clinical chemistry.

[6]  G. Gerber,et al.  The determination of nicotinuric acid by means of the azlactone reaction. , 1966, Clinica chimica acta; international journal of clinical chemistry.

[7]  G. Gerber,et al.  STUDIES ON DETOXICATION BY THE PERFUSED LIVER. I. HIPPURIC ACID SYNTHESIS AFTER IRRADIATION. , 1963, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[8]  J. Scaife.  Effect of ionizing radiation on the pyridine nucleotides of thymocytes. , 1963, Canadian journal of biochemistry and physiology.

[9]  D. K. Myers.  Synthesis of Diphosphopyridine Nucleotide in Irradiated Rats , 1960, Nature.

[10]  M. Sahasrabudhe,et al.  Synthesis of N'-methylnicotinamide in x-irradiated rats. , 1960, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[11]  P. Handler,et al.  Biosynthesis of diphosphopyridine nucleotide. I. Identification of intermediates. , 1958, The Journal of biological chemistry.