Functional localization in the cerebellar cortex studied by quantitative determinations of purkinje cell RNA. I. RNA changes in rat cerebellar purkinje cells after proprio--and exteroceptive and vestibular stimulation.

The effects of proprioceptive and exteroceptive stimulation and of vestibular stimulation on cerebellar Purkinje cell RNA content and composition were studied in male albino rats. After stimulation involving extero-and proprioceptors a significant increase of RNA was found in the vermian part of the lobulus centralis, the pyramis, the copula pyramidis and the parafloculus. No such increase was found in the lateral parts of the lobulus centralis, lateral parts of the culmen, the simplex, crus I or in the nodulus. After vestibular stimulation an increase of RNA content occurred in the lobulus centralis and the nodulus but not in the pyramis, the copula pyramidis or in the paraflocculus. A significant decrease of the purine/pyrimidine quotient was found in the vermian part of the lobulus centralis and in the nodulus of rats receiving vestibular stimulation. A comparison between the neuroanatomical and electrophysiological findings in this field has been presented. The conclusions are drawn that Purkinje cells increase their RNA content upon stimulation and that this condition may be used for studies of functional localization in the cerebellum.

[1]  W. Grampp,et al.  NERVOUS ACTIVITY AND METABOLISM OF RIBONUCLEIC ACIDS IN THE CRUSTACEAN STRETCH RECEPTOR NEURON , 1965, Journal of neurochemistry.

[2]  O. Oscarsson,et al.  FUNCTIONAL ORGANIZATION OF THE SPINO- AND CUNEOCEREBELLAR TRACTS. , 1965, Physiological reviews.

[3]  A. Brodal,et al.  SITE AND MODE OF TERMINATION OF PRIMARY VESTIBULOCEREBELLAR FIBRES IN THE CAT. AN EXPERIMENTAL STUDY WITH SILVER IMPREGNATION METHODS. , 1964, Archives italiennes de biologie.

[4]  W. Grampp,et al.  THE EFFECT OF NERVOUS ACTIVITY ON RIBONUCLEIC ACID OF THE CRUSTACEAN RECEPTOR NEURON , 1963, Journal of neurochemistry.

[5]  J. Jarlstedt.  The distribution of RNA in the cerebellum of the rabbit. , 1962, Experimental cell research.

[6]  H. Hydén,et al.  Nuclear RNA changes of nerve cells during a learning experiment in rats. , 1962, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Lundberg,et al.  Functional organization of the ventral spino-cerebellar tract in the cat. IV. Identification of units by antidromic activation from the cerebellar cortex. , 1962, Acta physiologica Scandinavica.

[8]  H. Hydén,et al.  EXPERIMENTALLY INDUCED CHANGES IN THE BASE COMPOSITION OF THE RIBONUCLEIC ACIDS OF ISOLATED NERVE CELLS AND THEIR OLIGODENDROGLIAL CELLS , 1961, The Journal of biophysical and biochemical cytology.

[9]  A. Lundberg,et al.  Functional organization of the dorsal spino-cerebellar tract in the cat. VII. Identification of units by antidromic activation from the cerebellar cortex with recognition of five functional subdivisions. , 1960, Acta physiologica Scandinavica.

[10]  H. Hydén,et al.  A CYTOPHYSIOLOGICAL STUDY OF THE FUNCTIONAL RELATIONSHIP BETWEEN OLIGODENDROGLIAL CELLS AND NERVE CELLS OF DEITERS' NUCLEUS , 1960, Journal of neurochemistry.

[11]  A. Lundberg,et al.  Functional organization of the dorsal spino-cerebellar tract in the cat. VI. Further experiments on excitation from tendon organ and muscle spindle afferents. , 1960, Acta physiologica Scandinavica.

[12]  W. Chambers,et al.  Functional localization in the cerebellum. II. Somatotopic organization in cortex and nuclei. , 1955, A.M.A. archives of neurology and psychiatry.

[13]  O. Larsell,et al.  The morphogenesis and adult pattern of the lobules and fissures of the cerebellum of the white rat , 1952, The Journal of comparative neurology.

[14]  B. Gernandt.  Response of mammalian vestibular neurons to horizontal rotation and caloric stimulation. , 1949, Journal of neurophysiology.

[15]  Robert F. Anderson.  Cerebellar distribution of the dorsal and ventral spino‐cerebellar tracts , 1943 .

[16]  Robert S. Dow,et al.  CEREBELLAR ACTION POTENTIALS IN RESPONSE TO STIMULATION OF VARIOUS AFFERENT CONNECTIONS , 1939 .

[17]  J. Jarlstedt.  Functional localization in the cerebellar cortex studied by quantitative determinations of Purkinje cell RNA. II. RNA changes in rabbit cerebellar Purkinje cells after caloric stimulation and vestibular neurotomy. , 1966, Acta physiologica Scandinavica. Supplementum.

[18]  A. Hamberger,et al.  CYTOCHEMICAL RESPONSE TO ACOUSTIC STIMULI IN THE SPIRAL GANGLION CELLS OF GUINEA PIGS. , 1965, Acta oto-laryngologica.

[19]  G. Grant,et al.  Spinal course and somatotopically localized termination of the spinocerebellar tracts. An experimental study in the cat. , 1962, Acta physiologica Scandinavica. Supplementum.

[20]  J. Jansen,et al.  Cerebellar corticovestibular fibers in the cat. , 1961, Experimental neurology.

[21]  S. Andersson,et al.  Cortical projection of vestibular nerve in cat. , 1954, Acta oto-laryngologica. Supplementum.

[22]  R. A. Fisher,et al.  Statistical methods for research workers. Biological monographs and manuals. No. V. , 1950 .