Automated analysis of flow cytometric data for measuring neutrophil CD64 expression using a multi‐instrument compatible probability state model

Leuko64TM (Trillium Diagnostics) is a flow cytometric assay that measures neutrophil CD64 expression and serves as an in vitro indicator of infection/sepsis or the presence of a systemic acute inflammatory response. Leuko64 assay currently utilizes QuantiCALC, a semiautomated software that employs cluster algorithms to define cell populations. The software reduces subjective gating decisions, resulting in interanalyst variability of <5%. We evaluated a completely automated approach to measuring neutrophil CD64 expression using GemStoneTM (Verity Software House) and probability state modeling (PSM).

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