Biosafe Ginseng: A Novel Source for Human Well‐Being

Given the enormous biodiversity in field cultivation, bioengineers were only to look within nature to find new promising phytomolecules or biochemical pathways from plant biofactories. The present review discusses pilot‐scale bioreactor cultivation of adventitious roots of ginseng and the enhanced production of pesticide‐free bioactive phytomolecules. This biotechnological method is described together with a discussion on economic and biosafety aspects. Besides the “classical” tissue culture as a subject of research on ginseng, new developments and efforts in pilot‐scale bioreactor tissue modeling and in the generation of artificial adventitious roots are considered. Additionally, recent progress in the field of medicinal and nutritional roles of adventitious roots produced in a pilot‐scale bioreactor are discussed.

[1]  Kee-Yoeup Paek,et al.  Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality , 2005 .

[2]  G. Sivakumar,et al.  Methyl Jasmonate Induce Enhanced Production of Soluble Biophenols in PANAX GINSENG Adventitious Roots from Commercial Scale Bioreactors , 2005, Chemistry of Natural Compounds.

[3]  G. Sivakumar,et al.  Production of Biomass and Ginsenosides from Adventitious Roots of Panax ginseng in Bioreactor Cultures , 2005 .

[4]  W. Tang.  High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng , 2000, Plant Cell Reports.

[5]  G. Sivakumar,et al.  Optimization of organic nutrients for ginseng hairy roots production in large-scale bioreactors , 2005 .

[6]  J. Zhong,et al.  Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. , 1999, Journal of biotechnology.

[7]  S. Kanba,et al.  Ginseng enhances contextual fear conditioning and neurogenesis in rats , 2005, Neuroscience Research.

[8]  M. Bonfill,et al.  Influence of auxins on organogenesis and ginsenoside production in Panax ginseng calluses , 2004, Plant Cell, Tissue and Organ Culture.

[9]  H. N. Murthy,et al.  Organic germanium stimulates the growth of ginseng adventitious roots and ginsenoside production , 2005 .

[10]  Kee-Yoeup Paek,et al.  Application of bioreactor systems for large scale production of horticultural and medicinal plants , 2005 .

[11]  H. N. Murthy,et al.  Methyl jasmonate elicitation enhanced synthesis of ginsenoside by cell suspension cultures of Panax ginseng in 5-l balloon type bubble bioreactors , 2005, Applied Microbiology and Biotechnology.

[12]  Y. Shoyama,et al.  Somatic Embryogenesis and Clonal Multiplication of Panax ginseng. , 1988, Planta medica.

[13]  K. Jeong,et al.  Panax ginseng prevents apoptosis in hair follicles and accelerates recovery of hair medullary cells in irradiated mice. , 1998, In vivo.

[14]  Mun Yhung Jung,et al.  Free, esterified, and insoluble-bound phenolic acids in white and red Korean ginsengs (Panax ginseng C.A. Meyer) , 2002 .

[15]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[16]  S. M. Choi,et al.  Pilot-scale culture of adventitious roots of ginseng in a bioreactor system , 2000, Plant Cell, Tissue and Organ Culture.

[17]  J. Zhong,et al.  Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium , 1998 .

[18]  J. Zhong,et al.  Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng , 1999 .

[19]  Jang Ryol Liu,et al.  Agrobacterium-mediated transformation of ginseng (Panax ginseng) and mitotic stability of the inserted β-glucuronidase gene in regenerants from isolated protoplasts , 1995, Plant Cell Reports.

[20]  S. Arya,et al.  Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis , 1991, Plant Cell Reports.

[21]  I. D. Arya,et al.  Rapid multiplication of adventitious somatic embryos of Panax ginseng , 1993, Plant Cell, Tissue and Organ Culture.

[22]  Paroma Basu.  Trading on traditional medicines , 2004, Nature Biotechnology.

[23]  Y. Gozu,et al.  Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots , 1993, Plant Cell Reports.

[24]  Y. Lee,et al.  Differences in immunomodulating effects between wild and cultured Panax ginseng. , 1994, Biochemical and biophysical research communications.

[25]  J. Zhong,et al.  Simultaneous production of ginseng saponin and polysaccharide by suspension cultures of Panax ginseng: Nitrogen effects , 1997 .