Changes in the levels of glutathione after cellular and cutaneous damage induced by squalene monohydroperoxide

Squalene monohydroperoxide (Sq‐OOH), the initial product of ultraviolet‐peroxidated squalene, was used to investigate the effect of peroxidative challenge upon the glutathione contents in rabbit ear skin and primary‐cultured fibroblasts derived from rabbit ear skin. The cellular reduced glutathione (GSH) contents decreased during 30‐minute incubations in vitro with Sq‐OOH, and oxidized glutathione (GSSG) was formed concomitantly, indicating that Sq‐OOH had a potential for GSH‐depleting activity in vitro. When Sq‐OOH was applied topically to the skin in vivo, only GSSG contents increased significantly within 30 minutes. Moreover, pretreatment with the GSH depletors, DL‐buthionine sulfoximine (BSO) and diethyl maleate (DEM), could potentiate the cytotoxicity and comedogenicity induced by Sq‐OOH. These findings suggest that the endogenous antioxidant, glutathione, is quite sensitive to Sq‐OOH and may be an important material for protecting cells and/or tissues against the oxidative stress induced by Sq‐OOH treatment. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:150–158, 2001

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