Translational applications of adult stem cell-derived organoids

ABSTRACT Adult stem cells from a variety of organs can be expanded long-term in vitro as three-dimensional organotypic structures termed organoids. These adult stem cell-derived organoids retain their organ identity and remain genetically stable over long periods of time. The ability to grow organoids from patient-derived healthy and diseased tissue allows for the study of organ development, tissue homeostasis and disease. In this Review, we discuss the generation of adult stem cell-derived organoid cultures and their applications in in vitro disease modeling, personalized cancer therapy and regenerative medicine. Summary: This Primer article discusses how adult stem cell-derived organoids can be used to model human diseases, create personalized cancer therapies and further efforts in regenerative medicine.

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