Antioxidant enzymes status in south indian hemodialysis patients

Abstract: Free radicals occupy a pivotal role among the atherosclerosis explanations.An imbalance in favor of pro-oxidants can lead to the oxidation of macromolecules resulting in tissue injury.27 patients of ESRD on maintenance haemodialysis were included and blood samples were collected at the start of haemodialysis (prehd), at 1 hr, 2hrs, 3hrs and at the end of the dialysis procedure (post HD).Plasma MDA estimated as TBARS (Thiobarbituric acid reactive substance),erythrocyte Super Oxide Dismutase (SOD), Glutathione reductase, Glutathione Peroxidase (GPx),Catalase Continuous Spectrophotometric rate determination. A significant decrease in malondialdehyde levels (p=0.017) were observed which however on correction for clearance, SOD, GR and Catalase showed an increase in activity (p<0.001) as a result of dialysis. A significant decrease in malondialdehyde levels (p=0.017) were observed which however on correction for clearance, as shown in Figure: 21 A was found to be increased (p=0.001).No change in GPx activity (p=0.704) was observed as a result of dialysis. Hemodialysis per se has been suggested to induce oxidative stress, with reactive oxygen species being generated on the surface of dialysis membranes by activation of polymorphonuclear leukocytes. Ongoing oxidative stress present in the patients on MHD may play a pathophysiological role in the development of cardiovascular disease. HD is mainly responsible for free radical production as well as non-enzymatic antioxidant losses.

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