High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform.

Three-dimensional (3D) tumor culture miniaturized platforms are of importance to biomimetic model construction and pathophysiological studies. Controllable and high-throughput production of 3D tumors is desirable to make cell-based manipulation dynamic and efficient at micro-scale. Moreover, the 3D culture platform being reusable is convenient to research scholars. In this chapter, we describe a dynamically controlled 3D tumor manipulation and culture method using pneumatic microstructure-based microfluidics, which has potential applications in the fields of tissue engineering, tumor biology, and clinical medicine in a high-throughput way.

[1]  Nicolas Bremond,et al.  Cellular capsules as a tool for multicellular spheroid production and for investigating the mechanics of tumor progression in vitro , 2013, Proceedings of the National Academy of Sciences.

[2]  S. Takayama,et al.  Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[3]  Tianbao Li,et al.  Monitoring tumor response to anticancer drugs using stable three-dimensional culture in a recyclable microfluidic platform. , 2015, Analytical chemistry.

[4]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[5]  R. Kwapiszewski,et al.  A microfluidic-based platform for tumour spheroid culture, monitoring and drug screening. , 2014, Lab on a chip.

[6]  Qin Tu,et al.  An integrated microfluidic system for studying cell-microenvironmental interactions versatilely and dynamically. , 2010, Lab on a chip.

[7]  K. Cheung,et al.  Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing. , 2010, Lab on a chip.

[8]  David J Beebe,et al.  Microfluidic 3D models of cancer. , 2014, Advanced drug delivery reviews.

[9]  K Dane Wittrup,et al.  Quantitative spatiotemporal analysis of antibody fragment diffusion and endocytic consumption in tumor spheroids. , 2008, Cancer research.

[10]  S. Quake,et al.  Versatile, fully automated, microfluidic cell culture system. , 2007, Analytical chemistry.

[11]  Qin Tu,et al.  Dynamic trapping and high-throughput patterning of cells using pneumatic microstructures in an integrated microfluidic device. , 2012, Lab on a chip.

[12]  C. V. van Blitterswijk,et al.  Spheroid culture as a tool for creating 3D complex tissues. , 2013, Trends in biotechnology.

[13]  J. Jessup,et al.  Nitrosative stress in rotated three-dimensional colorectal carcinoma cell cultures induces microtubule depolymerization and apoptosis. , 2004, Cancer research.

[14]  K. Oh,et al.  Generation of core-shell microcapsules with three-dimensional focusing device for efficient formation of cell spheroid. , 2011, Lab on a chip.

[15]  Genee Y. Lee,et al.  Three-dimensional culture models of normal and malignant breast epithelial cells , 2007, Nature Methods.