Evaluation of in vitro cytotoxicity of tetramethylarsonium hydroxide in marine animals

We have studied the cytotoxicity in vitro of tetramethylarsonium hydroxide (TetMA-OH), which is found in some marine animals, in various murine immune effector cells, including splenocytes, thymocytes, Peyer's patch (PP) lymphocytes, peritoneal macrophages (PMs) alveolar macrophages (AMs) and bone-marrow (BM) cells, using synthetic material which was compared with an inorganic arsenical, sodium arsenite. Arsenite showed strong cytotoxicity in these cells, with an IC 50 (the concentration that reduced the number of surviving cells to 50% of that in untreated controls) of about 2-9 μmol dm -3 . In contrast, TetMA-OH was less toxic, even at a concentration above 10 mmol dm -3 , in these immune effector cells, and no enhancement effect on the viability of the cells was observed. These data suggested that TetMA-OH had no biological effect, either toxic or modulating on any immune effector cells in vitro.

[1]  T. Saitoh,et al.  Biotransformation of arsenobetaine to trimethylarsine oxide by marine microorganisms in a gill of clam Meretrix lusoria , 1998 .

[2]  T. Saitoh,et al.  Study of in vitro cytotoxicity of a water soluble organic arsenic compound, arsenosugar, in seaweed. , 1997, Toxicology.

[3]  X. Hou,et al.  Determination of chemical species of iodine in some seaweeds (I) , 1997 .

[4]  T. Sakurai,et al.  Immunotoxicity of Organic Arsenic Compounds in Marine Animals , 1996 .

[5]  N. Ohno,et al.  Effects of fungal β‐glucan and interferon‐γ on the secretory functions of murine alveolar macrophages , 1996, Journal of leukocyte biology.

[6]  K. Uchida,et al.  Uptake and degradation of arsenobetaine by the microorganisms occurring in sediments , 1995 .

[7]  T. Kaise,et al.  Degradation of a tetramethylarsonium salt by microorganisms occurring in sediments and suspended substances under both aerobic and anaerobic conditions , 1994 .

[8]  S. Ahmed,et al.  A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. , 1994, Journal of immunological methods.

[9]  X. Le,et al.  Human urinary arsenic excretion after one-time ingestion of seaweed, crab, and shrimp. , 1994, Clinical chemistry.

[10]  N. Ohno,et al.  Intravenously administered (1----3)-beta-D-glucan, SSG, obtained from Sclerotinia sclerotiorum IFO 9395 augments murine peritoneal macrophage functions in vivo. , 1992, Chemical & pharmaceutical bulletin.

[11]  N. Ohno,et al.  Enhancement of murine alveolar macrophage functions by orally administered beta-glucan. , 1992, International journal of immunopharmacology.

[12]  T. Kaise,et al.  The chemical form and acute toxicity of arsenic compounds in marine organisms , 1992 .

[13]  T. Ishii,et al.  Enhancement of glutathione levels in mouse peritoneal macrophages by sodium arsenite, cadmium chloride and glucose/glucose oxidase. , 1991, Biochimica et biophysica acta.

[14]  T. Tani,et al.  A comparative study on acute toxicity of methylarsonic acid, dimethylarsinic acid and trimethylarsine oxide in mice , 1989 .

[15]  T. Kaise,et al.  Distribution of inorganic arsenic and methylated arsenic in marine organisms , 1988 .

[16]  K. Shiomi,et al.  Acute toxicity and rapid excretion in urine of tetramethylarsonium salts found in some marine animals , 1988 .

[17]  H. Yamanaka,et al.  Identification of arsenobetaine and a tetramethylarsonium salt in the clam Meretrix lusoria , 1987 .

[18]  T. Kaise,et al.  The acute toxicity of arsenobetaine , 1985 .

[19]  C. Raston,et al.  Isolation, crystal structure and synthesis of arsenobetaine, the arsenical constituent of the western rock lobster panulirus longipes cygnus George , 1977 .