Phylogenetic divergence in leatherside chub (Gila copei) inferred from mitochondrial cytochrome b sequences

We examined intra‐specific phylogenetic relationships in leatherside chub, Gila copei. The complete mitochondrial (mt) cytochrome b gene (1140 bp) was sequenced for 30 individuals from 10 populations that span the geographical distribution of this species. Traditional phylogenetic analyses revealed two deeply divergent and evolutionarily distinct mtDNA clades that are geographically separated in northern and southern drainage basins. Inter‐population sequence variation between clades ranged from 7.7 to 8.1%. The northern clade was genetically more similar and phylogenetically more closely related to the selected out‐group Lepidomeda m. mollispinus than to the southern clade, suggesting that the taxonomy of this species may require revision. Sequence variation among populations within clades ranged from 0 to 0.3% in the north and from 0 to 0.7% in the south. Statistical parsimony was used to construct phylogenetic networks of haplotypes within clades. Nested clade analysis revealed that geographical fragmentation has played an important role in genetic structuring within northern and southern clades.

[1]  K. Crandall,et al.  GeoDis: a program for the cladistic nested analysis of the geographical distribution of genetic haplotypes , 2000, Molecular ecology.

[2]  Pamela S. Soltis,et al.  Molecular Systematics and the Conservation of Rare Species , 1999 .

[3]  Carol A. Stepien,et al.  Molecular systematics of fishes , 1998 .

[4]  Templeton,et al.  Nested clade analyses of phylogeographic data: testing hypotheses about gene flow and population history , 1998, Molecular ecology.

[5]  S. Haig.  MOLECULAR CONTRIBUTIONS TO CONSERVATION , 1998 .

[6]  T. Dowling,et al.  Evolutionary Relationships of Minnows in the Genus Luxilus (Teleostei: Cyprinidae) as Determined from Cytochrome b Sequences , 1997 .

[7]  Richard L. Mayden,et al.  Phylogenetic Relationships of the Creek Chubs and the Spine‐Fins: an Enigmatic Group of North American Cyprinid Fishes (Actinopterygii: Cyprinidae) , 1997, Cladistics : the international journal of the Willi Hennig Society.

[8]  D. Swofford,et al.  Evolution of the Mitochondrial Cytochrome Oxidase II Gene in Collembola , 1997, Journal of Molecular Evolution.

[9]  W. Moore.  INFERRING PHYLOGENIES FROM mtDNA VARIATION: MITOCHONDRIAL‐GENE TREES VERSUS NUCLEAR‐GENE TREES , 1995, Evolution; international journal of organic evolution.

[10]  A. Templeton,et al.  Separating population structure from population history: a cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the tiger salamander, Ambystoma tigrinum. , 1995, Genetics.

[11]  C. Moritz.  Defining 'Evolutionarily Significant Units' for conservation. , 1994, Trends in ecology & evolution.

[12]  K. Crandall.  Intraspecific Cladogram Estimation: Accuracy at Higher Levels of Divergence , 1994 .

[13]  A. Templeton,et al.  Root probabilities for intraspecific gene trees under neutral coalescent theory. , 1994, Molecular phylogenetics and evolution.

[14]  C. Sing,et al.  A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. IV. Nested analyses with cladogram uncertainty and recombination. , 1993, Genetics.

[15]  Kevin C. Nixon,et al.  Populations, Genetic Variation, and the Delimitation of Phylogenetic Species , 1992 .

[16]  Robert M. May,et al.  Taxonomy as destiny , 1990, Nature.

[17]  M. Nei,et al.  Relationships between gene trees and species trees. , 1988, Molecular biology and evolution.

[18]  E. Boerwinkle,et al.  A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. I. Basic theory and an analysis of alcohol dehydrogenase activity in Drosophila. , 1987, Genetics.

[19]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[20]  Joseph Felsenstein,et al.  Statistical inference of phylogenies , 1983 .

[21]  M. Belk,et al.  Habitat characteristics of leatherside chub ( Gila copei ) at two spatial scales , 2001 .

[22]  P. Forey.  Molecular systematics of fishes , 1999 .

[23]  P. Lewis.  Maximum Likelihood as an Alternative to Parsimony for Inferring Phylogeny Using Nucleotide Sequence Data , 1998 .

[24]  K. Crandall,et al.  Multiple interspecies transmissions of human and simian T-cell leukemia/lymphoma virus type I sequences. , 1996, Molecular biology and evolution.

[25]  W. F. Sigler,et al.  Fishes of Utah: A Natural History , 1996 .

[26]  M. Belk,et al.  Age, growth, and reproduction of leatherside chub ( Gila copei ) , 1995 .

[27]  Gila Copei.  AGE, GROWTH, AND REPRODUCTION OF LEATHERSIDE CHUB , 1995 .

[28]  A. Templeton,et al.  The role of molecular genetics in speciation studies. , 1994, EXS.

[29]  Donald R. Currey.  Quaternary palaeolakes in the evolution of semidesert basins, with special emphasis on Lake Bonneville and the Great Basin, U.S.A. , 1990 .

[30]  John Alroy,et al.  Principles of genealogical concordance in species concepts and biological taxonomy , 1990 .

[31]  R. D. Jarrett,et al.  Paleodischarge of the late Pleistocene Bonneville Flood, Snake River, Idaho, computed from new evidence , 1987 .

[32]  Gerald R. Smith.  Biogeography of Intermountain fishes , 1978 .