论文信息 - Competitive interactions of adriamycin and ethidium bromide with DNA as studied by full rank parallel factor analysis of fluorescence three-way array data.

Competitive interactions of adriamycin and ethidium bromide with DNA as studied by full rank parallel factor analysis of fluorescence three-way array data.

The competitive interactions of adriamycin (AMC) and a fluorescence probe of ethidium bromide (EB) with DNA have been studied by full rank parallel factor analysis (FRA-PARAFAC) of fluorescence excitation-emission three-way data array. The excitation and emission spectra as well as the equilibrium concentrations of co-existing species in different reaction mixtures can be directly obtained by the FRA-PARAFAC treatment. The concordance of the resolved excitation and emission spectra of AMC, EB and EB-DNA with the standard spectra of these species confirmed the reliability of the equilibrium concentrations of these components in the reaction mixtures studied. The results obtained are valuable for providing a deeper insight into the competitive interaction mechanism of AMC and EB with DNA. The conclusion was directly given out that the interaction of AMC with DNA is the intercalating model. The FRA-PARAFAC method as exemplified by the present study provides an useful approach for studying the interaction of clinical drugs with DNA in the presence of disturbance of drug assistants.

[1] J. Lepecq,et al. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. , 1967, Journal of molecular biology.

[2] J. R. Brown,et al. The interaction of adriamycin and adriamycin analogues with nucleic acids in the B and A conformations. , 1979, Biochimica et biophysica acta.

[3] G. Smulevich,et al. Absorption, fluorescence and resonance Raman spectra of adriamycin and its complex with DNA , 1982 .

[4] R. Decker,et al. Binding of daunomycin to DNA and the inhibition of RNA and DNA synthesis. , 1975, Cancer research.

[5] T Theophanides,et al. Interaction of adriamycin with DNA as studied by resonance Raman spectroscopy. , 1982, Nucleic acids research.

[6] N. Turro,et al. Using Ethidium Bromide To Probe the Interactions between DNA and Dendrimers , 2000 .

[7] J. Chaires,et al. CONTRASTING HYDRATION CHANGES FOR ETHIDIUM AND DAUNOMYCIN BINDING TO DNA , 1999 .

[8] D. Ward,et al. Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs , 1965, Science.

[9] R. Bro. PARAFAC. Tutorial and applications , 1997 .

[10] F. Arcamone,et al. Adriamycin, 14‐hydroxydaimomycin, a new antitumor antibiotic from S. Peucetius var. caesius , 1969, Biotechnology and bioengineering.