OPTIMAL RESOLUTION FOR AUTOMATIC QUANTIFICATION OF BLOOD VESSELS ON DIGITIZED IMAGES OF THE WHOLE CANCER SECTION

Discrepancies concerning the prognostic significance of cancer vascularization can be partly explained by biases due to quantification protocols. We recently recommended a swift, inexpensive and automatic analysis of 2,700 dpi slide scanner images of the whole immunostained sections. Another team, proposed, quite at the same time, to work at 4,000 dpi. The aim of the present paper is to check if information contained in images scanned at 2,700 and 4,000 dpi are relevant and equivalent, when compared to the low magnification of the microscope, in order to propose the best compromise between precision and time expense. To evaluate precisely the amount of information gained or lost according to the resolution used, we compared the number and size of blood vessel profiles, manually detected, on twenty one Hodgkin lymphoma acquired with a scanner (2,700 and 4,000 dpi) and with a microscope (16,000 dpi). Results obtained at 4,000 dpi were equivalent to the estimation performed at microscopical level either by a biologist or a pathologist, while tiny vessels were lost at 2,700 dpi. Scanning whole histological sections at 4,000 dpi provides a relevant method for evaluating tumour vascularization, which can be easily automated and standardized.

[1]  J. Monson,et al.  Is the relationship between angiogenesis and metastasis in breast cancer real? , 1992, Surgical oncology.

[2]  S. Leurgans,et al.  Tumor angiogenesis in primary and metastatic colorectal cancers , 1996, Diseases of the colon and rectum.

[3]  M. Volm,et al.  Interrelationships between microvessel density, expression of VEGF and resistance to doxorubicin of non-small lung cell carcinoma. , 1996, Anticancer research.

[4]  J. Ferro,et al.  Angiogenesis in breast cancer is related to age but not to other prognostic parameters. , 1997, Pathology, research and practice.

[5]  C. Angeletti,et al.  Angiogenesis as a prognostic indicator of survival in non-small-cell lung carcinoma: a prospective study. , 1997, Journal of the National Cancer Institute.

[6]  R. Whitehouse,et al.  Tumor angiogenesis in stage II colorectal carcinoma: association with survival. , 1998, American journal of clinical pathology.

[7]  L. Ellis,et al.  Vessel counts and vascular endothelial growth factor expression in pancreatic adenocarcinoma. , 1998, European journal of cancer.

[8]  P. Rogers,et al.  Increased microvessel density in mucinous compared with malignant serous and benign tumours of the ovary. , 1998, British Journal of Cancer.

[9]  J A Beliën,et al.  Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer. , 1999, Journal of clinical pathology.

[10]  R. Eggers,et al.  Flat‐bed scanning as a tool for quantitative neuroimaging , 1999, Journal of microscopy.

[11]  W Vach,et al.  The prognostic value of angiogenesis by Chalkley counting in a confirmatory study design on 836 breast cancer patients. , 2000, Clinical cancer research : an official journal of the American Association for Cancer Research.

[12]  J. Scoazec.  Tumor angiogenesis , 2000, Annales de pathologie.

[13]  The 7th ESACP (European Society for Analytical Cellular Pathology) Congress. Caen, France. April 1-5, 2001. Abstracts. , 2001, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[14]  A. Loewy,et al.  High-resolution scanner for neuroanatomical analysis , 2002, Journal of Neuroscience Methods.

[15]  J. Verdebout,et al.  The role of microvessel density on the survival of patients with lung cancer: a systematic review of the literature with meta-analysis , 2002, British Journal of Cancer.

[16]  M Gion,et al.  Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. , 2002, European journal of cancer.

[17]  C. Perrett,et al.  Angiogenesis in epithelian ovarian cancer , 2002, Molecular pathology : MP.

[18]  G. Nagy,et al.  Physiological and pathological angiogenesis in the endocrine system , 2003, Microscopy research and technique.

[19]  Anju Relan,et al.  Self-instructional "virtual pathology" laboratories using web-based technology enhance medical school teaching of pathology. , 2003, Human pathology.

[20]  Fred R. Dee,et al.  Implementation of virtual microscope slides in the annual pathobiology of cancer workshop laboratory. , 2003, Human pathology.

[21]  H. Deissler,et al.  Angiogenesis: prognostic and therapeutic implications in gynecologic and breast malignancies , 2003, Current opinion in obstetrics & gynecology.

[22]  B. Plancoulaine,et al.  An Original Approach for Quantification of Blood Vessels on the Whole Tumour Section , 2003, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[23]  S. Groshen,et al.  Computerized Quantification of Tissue Vascularization Using High-resolution Slide Scanning of Whole Tumor Sections , 2003, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  Benoît Plancoulaine,et al.  A simple way of quantifying immunostained cell nuclei on the whole histologic section , 2003, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[25]  S. Abe,et al.  Tumor angiogenesis of non–small cell lung cancer , 2003, Microscopy research and technique.

[26]  N. Weidner,et al.  Prognostic value of intratumoral microvessel density, a measure of tumor angiogenesis, in node-negative breast carcinoma — results of a multiparametric study , 2004, Breast Cancer Research and Treatment.

[27]  Julie Morris,et al.  Assessment of Vascularity in Histological Sections: Effects of Methodology and Value as an Index of Angiogenesis in Breast Tumours , 1998, The Histochemical Journal.

[28]  E. van Marck,et al.  Influence of investigator experience and microscopic field size on microvessel density in node-negative breast carcinoma , 2004, Breast Cancer Research and Treatment.