Enhanced cellular response elicited by addition of amniotic fluid to alginate hydrogel-electrospun silk fibroin fibers for potential wound dressing application.

This study aimed to evaluate a novel bioactive wound dressing from alginate hydrogel-electrospun silk fibroin (SF) fibers with the ability to deliver amniotic fluid (AF) to the wound site. AF is highly enriched with multiple therapeutic agents that can promote cellular response and wound healing. In this study, electrospun SF fibers were first fabricated by electrospinning method and then combined with the alginate hydrogel (ALG) containing AF. Different dressings were made by changing the alginate to AF ratio. The scanning electron microscopy images revealed that SF fibers were fully covered with alginate hydrogel. In addition, the modulus of the dressing decreased by lowering ALG/AF ratios, while a reverse trend was observed for the elongation-at-break. In vitro AF release profiles indicated that an increase in the concentration of ALG leads to a reduction in the AF amount. Fibroblast culturing on the fabricated dressings demonstrated that cellular proliferation, spreading, and secretion of collagen enhanced with increasing AF. Taken together, our results provide a novel bioactive dressing with great potentials for speeding up the healing process in severe wounds.

[1]  Jhamak Nourmohammadi,et al.  How direct electrospinning in methanol bath affects the physico-chemical and biological properties of silk fibroin nanofibrous scaffolds , 2016 .

[2]  Ran Yin,et al.  Alginate/chitosan based bi-layer composite membrane as potential sustained-release wound dressing containing ciprofloxacin hydrochloride , 2014 .

[3]  David L Kaplan,et al.  Silk as a Biomaterial. , 2007, Progress in polymer science.

[4]  Shweta Tripathi,et al.  Advances in Skin Regeneration Using Tissue Engineering , 2017, International journal of molecular sciences.

[5]  Qingquan Liu,et al.  Fabrication and Physical Properties of Gelatin/Sodium Alginate/Hyaluronic Acid Composite Wound Dressing Hydrogel , 2014 .

[6]  Paul P. Bonvallet,et al.  Microporous Dermal-Mimetic Electrospun Scaffolds Pre-Seeded with Fibroblasts Promote Tissue Regeneration in Full-Thickness Skin Wounds , 2015, PloS one.

[7]  S. Ramakrishna,et al.  Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration , 2014, International journal of nanomedicine.

[8]  Elbadawy A. Kamoun,et al.  L-Arginine grafted alginate hydrogel beads: A novel pH-sensitive system for specific protein delivery , 2015 .

[9]  W. Chae,et al.  Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo , 2017, International journal of molecular sciences.

[10]  F. Gu,et al.  Sustained delivery of vascular endothelial growth factor with alginate beads. , 2004, Journal of controlled release : official journal of the Controlled Release Society.

[11]  A. Subramanian,et al.  Injectable and 3D Bioprinted Polysaccharide Hydrogels: From Cartilage to Osteochondral Tissue Engineering. , 2017, Biomacromolecules.

[12]  Michel Destrade,et al.  Characterization of the anisotropic mechanical properties of excised human skin. , 2013, Journal of the mechanical behavior of biomedical materials.

[13]  Mingxian Liu,et al.  Effects of halloysite nanotubes on physical properties and cytocompatibility of alginate composite hydrogels. , 2017, Materials science & engineering. C, Materials for biological applications.

[14]  Jhamak Nourmohammadi,et al.  Composite of porous starch-silk fibroin nanofiber-calcium phosphate for bone regeneration , 2015 .

[15]  Dukjoon Kim,et al.  Effect of polymer solution concentration on the swelling and mechanical properties of glycol chitosan superporous hydrogels , 2010 .

[16]  A. Boccaccini,et al.  Evaluation of Fibroblasts Adhesion and Proliferation on Alginate-Gelatin Crosslinked Hydrogel , 2014, PloS one.

[17]  J. de la Noüe,et al.  Factors affecting protein release from alginate-chitosan coacervate microcapsules during production and gastric/intestinal simulation. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[18]  H. Thu,et al.  Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing. , 2012, International journal of pharmaceutics.

[19]  P. Ferretti,et al.  An overview of the therapeutic potential of regenerative medicine in cutaneous wound healing , 2017, International wound journal.

[20]  S. Ramakrishna,et al.  Recent advancements in nanotechnological strategies in selection, design and delivery of biomolecules for skin regeneration. , 2016, Materials science & engineering. C, Materials for biological applications.

[21]  Hsin-Yi Lin,et al.  The influence of operating parameters on the drug release and anti-bacterial performances of alginate wound dressings prepared by three-dimensional plotting , 2012 .

[22]  M. Ozcan,et al.  Effects of human amniotic fluid on peritendinous adhesion formation and tendon healing after flexor tendon surgery in rabbits. , 2001, The Journal of hand surgery.

[23]  M. Nouri,et al.  Structural characterization and mechanical properties of electrospun silk fibroin nanofiber mats , 2010 .

[24]  M. Yassin,et al.  Surprising performance of alginate beads for the release of low‐molecular‐weight drugs , 2010 .

[25]  E. Balmayor,et al.  Targeted delivery as key for the success of small osteoinductive molecules. , 2015, Advanced drug delivery reviews.

[26]  Weifeng He,et al.  Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement , 2016, Scientific Reports.

[27]  M. Varedi,et al.  Activation of mitosis and angiogenesis in diabetes-impaired wound healing by processed human amniotic fluid. , 2014, The Journal of surgical research.

[28]  J. Evans,et al.  The preclinical evaluation of the water vapour transmission rate through burn wound dressings. , 1987, Biomaterials.

[29]  W. Gilbert,et al.  Amniotic Fluid: Not Just Fetal Urine Anymore , 2005, Journal of Perinatology.

[30]  Yubo Fan,et al.  Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration. , 2013, Biomaterials.

[31]  M. Minary‐Jolandan,et al.  Alginate-Collagen Fibril Composite Hydrogel , 2015, Materials.

[32]  P. Bornstein,et al.  Amniotic fluid fibronectin. Characterization and synthesis by cells in culture , 1978, The Journal of cell biology.

[33]  G. Filiz,et al.  Effects of human amniotic fluid on peripheral nerve scarring and regeneration in rats. , 2003, Journal of neurosurgery.

[34]  M. Kouchak,et al.  Chitosan and polyvinyl alcohol composite films containing nitrofurazone: preparation and evaluation , 2014, Iranian journal of basic medical sciences.

[35]  Jianhua Zhang,et al.  Composites of electrospun-fibers and hydrogels: A potential solution to current challenges in biological and biomedical field. , 2016, Journal of biomedical materials research. Part B, Applied biomaterials.

[36]  P. Ma,et al.  Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties. , 2001, Biomaterials.

[37]  Liang Xu,et al.  An ionically crosslinked hydrogel containing vancomycin coating on a porous scaffold for drug delivery and cell culture. , 2008, International journal of pharmaceutics.

[38]  V. Pillay,et al.  Drug release modulation from cross-linked calcium alginate microdiscs, 1: evaluation of the concentration dependency of sodium alginate on drug entrapment capacity, morphology, and dissolution rate. , 1998, Drug delivery.

[39]  A. Bahador,et al.  An investigation of electrospun Henna leaves extract-loaded chitosan based nanofibrous mats for skin tissue engineering. , 2017, Materials science & engineering. C, Materials for biological applications.

[40]  Cristina Maderuelo,et al.  Critical factors in the release of drugs from sustained release hydrophilic matrices. , 2011, Journal of controlled release : official journal of the Controlled Release Society.

[41]  Wee,et al.  Protein release from alginate matrices. , 1998, Advanced drug delivery reviews.

[42]  Eben Alsberg,et al.  Electrospun alginate nanofibers with controlled cell adhesion for tissue engineering. , 2010, Macromolecular bioscience.

[43]  J. Holmberg,et al.  Plasma and Amniotic Fluid Concentrations of Fibronectin During Normal Pregnancy , 1986, Obstetrics and gynecology.

[44]  J. Kong,et al.  Fourier transform infrared spectroscopic analysis of protein secondary structures. , 2007, Acta biochimica et biophysica Sinica.

[45]  G. Qiao,et al.  Wheat gluten-based renewable and biodegradable polymer materials with enhanced hydrophobicity by using epoxidized soybean oil as a modifier. , 2010, Carbohydrate research.

[46]  B. Sarmento,et al.  Development and characterization of crosslinked hyaluronic acid polymeric films for use in coating processes. , 2016, International journal of pharmaceutics.

[47]  Yahya E Choonara,et al.  A comprehensive review of advanced biopolymeric wound healing systems. , 2014, Journal of pharmaceutical sciences.

[48]  O. Catanzano,et al.  Advanced Therapeutic Dressings for Effective Wound Healing--A Review. , 2015, Journal of pharmaceutical sciences.

[49]  Andrés J. García,et al.  Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate. , 2017, Acta Biomaterialia.

[50]  W. Khalil,et al.  Drug–polymer interaction between glucosamine sulfate and alginate nanoparticles: FTIR, DSC and dielectric spectroscopy studies , 2016 .

[51]  D. Kletsas,et al.  Human amniotic fluid stimulates the proliferation of human fetal and adult skin fibroblasts: The roles of bFGF and PDGF and of the ERK and Akt signaling pathways , 2010, Wound Repair and Regeneration.

[52]  T. E. Abraham,et al.  pH sensitive alginate-guar gum hydrogel for the controlled delivery of protein drugs. , 2007, International journal of pharmaceutics.

[53]  D. Brett.  A review of moisture-control dressings in wound care. , 2006, Journal of wound, ostomy, and continence nursing : official publication of The Wound, Ostomy and Continence Nurses Society.

[54]  P. Koşucu,et al.  Effect of human amniotic fluid on bone healing. , 2005, The Journal of surgical research.

[55]  D. Mooney,et al.  Decoupling the dependence of rheological/mechanical properties of hydrogels from solids concentration , 2002 .

[56]  Wim E Hennink,et al.  Hydrogels for protein delivery. , 2012, Chemical reviews.

[57]  Aaron B. Baker,et al.  Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing , 2016, Front. Bioeng. Biotechnol..

[58]  Mohammad Ali Shokrgozar,et al.  Enhanced mechanical properties of thermosensitive chitosan hydrogel by silk fibers for cartilage tissue engineering. , 2013, Materials science & engineering. C, Materials for biological applications.

[59]  C. Li,et al.  Tailoring mechanical and antibacterial properties of chitosan/gelatin nanofiber membranes with Fe3O4 nanoparticles for potential wound dressing application , 2016 .

[60]  Modulation of poly (d,l-lactic acid) with amniotic fluid for improvement of the cell affinity , 2005 .

[61]  A. D. Di Pasqua,et al.  Preparation of alginate beads containing a prodrug of diethylenetriaminepentaacetic acid. , 2013, Carbohydrate polymers.

[62]  B. Atiyeh,et al.  Management of acute and chronic open wounds: the importance of moist environment in optimal wound healing. , 2002, Current pharmaceutical biotechnology.