An HRP-based amperometric biosensor fabricated by thermal inkjet printing

Abstract Direct inkjet printing of a complete and working amperometric biosensor for the detection of hydrogen peroxide, based on horseradish peroxidase (HRP), has been demonstrated. The device has been realized with a commercial printer. A thin layer of PEDOT:PSS, which was in turn covered with HRP, was inkjet printed on top of an ITO-coated glass slide. The active components of the device retained their properties after the thermal inkjet printing. The whole device has been encapsulated by means of a selectively permeable cellulose acetate membrane. The successful electron transfer between the PEDOT:PSS covered electrode and the enzyme has been demonstrated, and the biosensor evidenced very good sensitivity, in line with the best devices realized with other techniques, and a remarkable operational stability. This result paves the way for an extensive application of “biopolytronics”, i.e. the utilization of conductive/semiconductive polymers and biologically active molecules to design bioelectronic devices using a common PC, and exploiting normal commercial printers to print them out.

[1]  Tuan Vo-Dinh,et al.  Microarray sampling-platform fabrication using bubble-jet technology for a biochip system , 2001, Fresenius' journal of analytical chemistry.

[2]  E. Csöregi,et al.  Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring. , 2005, Biosensors & bioelectronics.

[3]  M. Matsumiya,et al.  Temperature dependence of kinetics and diffusion coefficients for ferrocene/ferricenium in ammonium-imide ionic liquids , 2006 .

[4]  David C Cullen,et al.  Development of a common biosensor format for an enzyme based biosensor array to monitor fruit quality. , 2003, Biosensors & bioelectronics.

[5]  Scott C. Brown,et al.  A three-dimensional osteochondral composite scaffold for articular cartilage repair. , 2002, Biomaterials.

[6]  G. Marr,et al.  Oxidation of ferrocene and some substituted ferrocenes in the presence of horseradish peroxidase , 1978 .

[7]  H. Le,et al.  Progress and Trends in Ink-jet Printing Technology , 1998, Journal of Imaging Science and Technology.

[8]  Ping-Hei Chen,et al.  Bubble growth and ink ejection process of a thermal ink jet printhead , 1997 .

[9]  R. Stallcup,et al.  Effects of Cs deposition on the field-emission properties of single-walled carbon-nanotube bundles , 2001 .

[10]  L. Setti,et al.  Horseradish peroxidase-catalyzed oxidative coupling of 3-methyl 2-benzothiazolinone hydrazone and methoxyphenols , 1998 .

[11]  E. Ivanova,et al.  Linear free-energy relationships and inverted Marcus region in the horseradish peroxidase-catalyzed oxidation of ferrocenes by hydrogen peroxide , 1999 .

[12]  C. M. Hart,et al.  Low-cost all-polymer integrated circuits , 1998, Proceedings of the 24th European Solid-State Circuits Conference.

[13]  D. Combes,et al.  Effect of polyols on fungal alpha-amylase thermostability , 1989 .

[14]  Huai-Sheng Wang,et al.  A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide , 2005, Sensors (Basel, Switzerland).

[15]  U. Schubert,et al.  Inkjet Printing of Polymers: State of the Art and Future Developments , 2004 .

[16]  Tatsuya Shimoda,et al.  Inkjet printing of polymer thin film transistors , 2003 .

[17]  Bin Chen,et al.  All-polymer RC filter circuits fabricated with inkjet printing technology , 2003 .

[18]  Tao Xu,et al.  Construction of high‐density bacterial colony arrays and patterns by the ink‐jet method , 2004, Biotechnology and bioengineering.

[19]  A. Roda,et al.  Protein microdeposition using a conventional ink-jet printer. , 2000, BioTechniques.

[20]  John A. Rogers,et al.  Printable organic and polymeric semiconducting materials and devices , 1999 .

[21]  A. Blanchard,et al.  High-density oligonucleotide arrays , 1996 .

[22]  L. Setti,et al.  An amperometric glucose biosensor prototype fabricated by thermal inkjet printing. , 2005, Biosensors & bioelectronics.

[23]  C. Piana,et al.  Thermal inkjet microdeposition of PEDOT:PSS on ITO-coated glass and characterization of the obtained film , 2004 .

[24]  Giovanna Marrazza,et al.  INK-JET PRINTING FOR THE FABRICATION OF AMPEROMETRIC GLUCOSE BIOSENSORS , 1992 .

[25]  B. Piro,et al.  A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films , 2001 .

[26]  C. Piana,et al.  Thermal Inkjet Technology for the Microdeposition of Biological Molecules as a Viable Route for the Realization of Biosensors , 2004 .