Morphological, Cytological and Molecular Evidence for Intersubgeneric F₁ Hybrid between Glycine max × G. tomentella

This study was carried out to demonstrate morphological, cytological and molecular evidence for intersubgeneric F₁ hybrid between Glycine tomentella and G. max cv. 'Baemkong'. Morphological features of F₁ plant for pistil and stamen, flower color and growth habit showed intermediate type between G. tomentella and G. max cv. 'Baemkong'. Chromosome number of F₁ plant was 2n=39, which explained the evidence of F₁ hybrid between G. tomentella (2n=38) and G. max cv. 'Baemkong' (2n=40). Polyacrylamide isoelectric focusing pattern for esterase and peroxidase also showed that the F₁ plant was true F₁ hybrid between G. tomentella and G. max cv. 'Baemkong'. From RAPD analysis, we identified that 62 primers showing bands in F₁ hybrid had both bands from G. tomentella and G. max cv. 'Baemkong', which suggested that this was true F₁ hybrid. Based on our results from morphological, cytological and molecular analyses, we suggest that the F₁ plant was true intersubgeneric hybrid between G. tomentella and G. max cv. 'Baemkong'. Our results still remain us further study to recover fertility of F₁ hybrids. The occurrence of maternal and/or paternal inheritance in F₁ hybrid from intersubgeneric cross between G. tomentella and G. max cv. 'Baemkong' need to be explained.