Nonphosphorylated neurofilament protein and calbindin immunoreactivity in layer III pyramidal neurons of human neocortex.

Subpopulations of pyramidal neurons in the neocortex have been shown to contain nonphosphorylated neurofilament protein (NPNFP) and calbindin D28K (Morrison et al., 1987; Campbell and Morrison, 1989; Hof et al., 1990; Kobayashi et al., 1990; Hof and Morrison, 1991; Mesulam and Geula, 1991). However, it is not known what relations, if any, exist between the pyramidal neurons containing each of these proteins. In this study, the expression of NPNFP and calbindin immunoreactivity was compared in six regions of human neocortex. Characteristic laminar patterns of immunoreactivity for each protein were seen in most regions examined, and both NPNFP- and calbindin-labeled pyramidal neurons were found in layer III. However, the pyramidal neurons labeled with NPNFP and calbindin differed in several respects. First, the sublaminar distribution of NPNFP-labeled pyramids within layer III differed across regions, ranging from an even distribution throughout the layer in a visual association region (area 18) to a predominance of labeled neurons in the deep half of that layer in a higher association region (area 20). The distribution of calbindin-immunoreactive pyramidal neurons also varied regionally, but in a different manner than that of the NPNFP-labeled neurons. Second, in every region examined, the average size of NPNFP-labeled layer III pyramids was greater than that of calbindin-immunoreactive pyramids. However, there was substantial regional heterogeneity in the extent to which the size distributions of neurons in each of the two populations overlapped. Third, in the regions in which NPNFP- and calbindin-immunoreactive neurons were most similar in size, the amount of colocalization (as identified by double-labeling studies) was also greatest. Similarly, in the regions in which there was minimal overlap in the size of the NPNFP- and the calbindin-immunoreactive neurons, there was minimal colocalization. These regional characteristics of NPNFP- and calbindin-immunoreactive layer III pyramidal neurons have implications for the involvement of these neuronal populations in Alzheimer's disease.

[1]  E. Baráth,et al.  Fundamentals of Biostatistics. , 1992 .

[2]  C. Geula,et al.  Differential distribution of a neurofilament protein epitope in acetylcholinesterase-rich neurons of human cerebral neocortex , 1991, Brain Research.

[3]  J. Morrison,et al.  Neocortical neuronal subpopulations labeled by a monoclonal antibody to calbindin exhibit differential vulnerability in Alzheimer's disease , 1991, Experimental Neurology.

[4]  J. Cameron,et al.  A comparative analysis of the distribution of prosomatostatin‐derived peptides in human and monkey neocortex , 1991, The Journal of comparative neurology.

[5]  J. Morrison,et al.  A subpopulation of primate corticocortical neurons is distinguished by somatodendritic distribution of neurofilament protein , 1991, Brain Research.

[6]  Kevin Cox,et al.  Quantitative analysis of a vulnerable subset of pyramidal neurons in Alzheimer's disease: I. Superior frontal and inferior temporal cortex , 1990, The Journal of comparative neurology.

[7]  J. Morrison,et al.  Quantitative analysis of a vulnerable subset of pyramidal neurons in Alzheimer's disease: II. Primary and secondary visual cortex , 1990, The Journal of comparative neurology.

[8]  T. Powell,et al.  The ipsilateral cortico-cortical connections of area 7b, PF, in the parietal and tempral lobes of the monkey , 1990, Brain Research.

[9]  S. Christakos,et al.  Specific reduction of calcium-binding protein (28-kilodalton calbindin-D) gene expression in aging and neurodegenerative diseases. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[10]  P. Emson,et al.  Cerebral cortical calbindin D28K and parvalbumin neurones in Down's syndrome , 1990, Neuroscience Letters.

[11]  J. Lund,et al.  Heterogeneity of chandelier neurons in monkey neocortex: Corticotropin‐releasing factor‐and parvalbumin‐immunoreactive populations , 1990, The Journal of comparative neurology.

[12]  E. G. Jones,et al.  Synapses of double bouquet cells in monkey cerebral cortex visualized by calbindin immunoreactivity , 1989, Brain Research.

[13]  D. Lewis,et al.  Antibodies directed against tyrosine hydroxylase differentially recognize noradrenergic axons in monkey neocortex , 1989, Brain Research.

[14]  A. McKee,et al.  CALBINDIN D28K IMMUNOREACTIVE NEURONS IN TEMPORAL ISOCORTEX RESIST DEGENERATION IN ALZHEIMERʼS DISEASE: 92 , 1989 .

[15]  J. Morrison,et al.  Monoclonal antibody to neurofilament protein (SMI‐32) labels a subpopulation of pyramidal neurons in the human and monkey neocortex , 1989, The Journal of comparative neurology.

[16]  P. Emson,et al.  Loss of calbindin-28K immunoreactive neurones from the cortex in Alzheimer-type dementia , 1988, Brain Research.

[17]  K Watanabe,et al.  Connections of area 8 with area 6 in the brain of the macaque monkey , 1988, The Journal of comparative neurology.

[18]  L. Otvos,et al.  Identification of the major multiphosphorylation site in mammalian neurofilaments. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[19]  John H. Morrison,et al.  A monoclonal antibody to non-phosphorylated neurofilament protein marks the vulnerable cortical neurons in Alzheimer's disease , 1987, Brain Research.

[20]  P S Goldman-Rakic,et al.  Callosal and intrahemispheric connectivity of the prefrontal association cortex in rhesus monkey: Relation between intraparietal and principal sulcal cortex , 1984, The Journal of comparative neurology.

[21]  P. Goldman-Rakic,et al.  Interdigitation of contralateral and ipsilateral columnar projections to frontal association cortex in primates. , 1982, Science.

[22]  D. V. van Essen,et al.  The pattern of interhemispheric connections and its relationship to extrastriate visual areas in the macaque monkey , 1982, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[23]  J. Lund,et al.  Anatomical organization of primate visual cortex area VII , 1981, The Journal of comparative neurology.

[24]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[25]  K. Rockland,et al.  Laminar origins and terminations of cortical connections of the occipital lobe in the rhesus monkey , 1979, Brain Research.