Fiber-delivered picosecond source for coherent Raman scattering imaging.

We demonstrate a two-color, fiber-delivered picosecond source for coherent Raman scattering (CRS) imaging. The wavelength-tunable picosecond pump is generated by nonlinear spectral compression of a prechirped femtosecond pulse from a mode-locked titanium:sapphire (Ti:S) laser. The 1064 nm picosecond Stokes pulse is generated by an all-fiber time-lens source that is synchronized to the Ti:S laser. The pump and Stokes beams are combined in an optical fiber coupler, which serves not only as the delivery fiber but also as the nonlinear medium for spectral compression of the femtosecond pulse. CRS imaging of mouse skin is performed to demonstrate the practicality of this source.

[1]  Gangjun Liu,et al.  Fiber delivered probe for efficient CARS imaging of tissues. , 2010, Optics express.

[2]  Christian W. Freudiger,et al.  Synchronized time-lens source for coherent Raman scattering microscopy , 2010, Optics express.

[3]  Kazuyoshi Itoh,et al.  Stimulated Raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses. , 2010, Optics express.

[4]  Mortazavi,et al.  Supporting Online Material Materials and Methods Figs. S1 to S13 Tables S1 to S3 References Label-free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy , 2022 .

[5]  Esben Ravn Andresen,et al.  Stimulated Raman scattering microscopy by spectral focusing and fiber-generated soliton as Stokes pulse. , 2011, Optics letters.

[6]  Annika Enejder,et al.  Nonlinear microscopy with fiber laser continuum excitation. , 2010, Journal of biomedical optics.

[7]  Jun Ye,et al.  High-sensitivity coherent anti-Stokes Raman scattering microscopy with two tightly synchronized picosecond lasers. , 2002, Optics letters.

[8]  Liang Dong,et al.  All-fiber CARS microscopy of live cells. , 2009, Optics express.

[9]  Markus K. Oberthaler,et al.  Special narrowing of ultrashort laser pulses by self‐phase modulation in optical fibers , 1993 .

[10]  X. Xie,et al.  Coherent Raman scanning fiber endoscopy. , 2011, Optics letters.

[11]  Demirhan Kobat,et al.  Tunable dispersion compensation by a rotating cylindrical lens. , 2009, Optics letters.

[12]  D. Côté,et al.  Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system , 2011, Biomedical optics express.

[13]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[14]  Alfred Leitenstorfer,et al.  Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system. , 2009, Optics letters.

[15]  N. Nishimura,et al.  Deep tissue multiphoton microscopy using longer wavelength excitation. , 2009, Optics express.

[16]  Khanh Kieu,et al.  High-power picosecond fiber source for coherent Raman microscopy. , 2009, Optics letters.

[17]  Esben Ravn Andresen,et al.  Transform-limited spectral compression by self-phase modulation of amplitude-shaped pulses with negative chirp. , 2011, Optics letters.